Abstract
Changes in haematological parameters and differential leukocyte counts of peripheral blood and head kidney haematopoietic tissue of mullet were assessed after an experimental anaemia, induced by caudal blood extraction. Thirty fish were used and divided into two groups: Group A (control group n = 20) and Group B (experimental group n = 10). From Group A, 10 fish were sacrificed to obtain head kidney control; from the 10 remaining, blood samples were collected by caudal venipuncture. From Group B, blood samples were collected five times once a week (T0, T1, T2, T3, T4) to induce experimental blood loss. At the end of the experimental period fish were sacrificed and the head kidneys were isolated. For all fish, haemogram and manual differential leukocyte counts were determined in peripheral blood. Reduced red blood cell count (RBC), haematocrit (Hct), haemoglobin (Hb), thrombocyte count (TC), lymphocytes and increased mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), monocytes and neutrophil values were found at T1 with respect to T0. Successively, until T3, RBC, Hct, Hb, TC and lymphocyte values showed an increase in peripheral blood. A decrease of haematological parameters in peripheral blood after the last bleeding time (T4) was observed. Cell identification and differential counts on head kidneys were performed and a significant increase of lymphocytes, monocytes and early unidentified blast cells was observed. A decrease in RBC, neutrophils, eosinophils, TC and a total absence of basophils were found. These results indicated that mullet are able to recover from anaemia caused by various adverse environmental and/or nutritional factors.
Acknowledgements
Animal experimental protocol was followed according to Directive 63/2010/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes. The authors would like to thank the Provincia of Messina for allowing the sampling. The authors declare that they have no conflict of interest.
Editorial responsibility: Göran Nilsson