Development of Kunitz Trypsin inhibitor free vegetable soybean genotypes through marker-assisted selection

ABSTRACT

Genetic elimination of Kunitz trypsin inhibitor (KTI) from vegetable soybean (VSB) (Glycine max L. Merrill.) is important as inactivation of this anti-nutritional factor requires 15–20 min. boiling. The F₂ plants carrying the null allele of KTI were identified using gene-specific markers in two populations generated by crossing two (VSB) genotypes with a null KTI grain-type genotype. Deployment of gene-specific and Ti-linked simple sequence repeat (SSR) markers led to identification of homozygous recessive (titi) lines in the F₆ generation. Across both populations, 21 F₆ KTI free lines exhibiting 100-fresh seed weight >45 g, sucrose content >7%, and morphologically similar to VSB were selected. Moisture content and pod yield, at R6, selected lines were 64–74.1% and 7.0−9.5 t∙ha⁻¹, respectively. This is the first report on development of KTI-free VSB which may be suitable for direct consumption.

KEYWORDS:

• Glycine max
• edamame
• seed weight
• sucrose

Acknowledgments

The authors thank Indian Council of Agricultural Research-Indian Institute of Soybean Research, Indore, for providing infrastructure to carry out this work.

Disclosure statement

The authors declare that they have no conflict of interest.

Submission declaration

This work has not been published previously, is not under consideration for publication elsewhere, and has not been simultaneously submitted elsewhere.