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Review

Amyloids and prions in plants: Facts and perspectives

K. S. AntonetsAll-Russia Research Institute for Agricultural Microbiology, St. Petersburg, Russian Federation;Department of Genetics and Biotechnology, St. Petersburg State University, St. Petersburg, Russian Federation
&
A. A. NizhnikovAll-Russia Research Institute for Agricultural Microbiology, St. Petersburg, Russian Federation;Department of Genetics and Biotechnology, St. Petersburg State University, St. Petersburg, Russian FederationCorrespondence[email protected]
ORCID Iconhttp://orcid.org/0000-0002-8338-3494
ORCID Icon
Pages 300-312 | Received 20 Jul 2017, Accepted 05 Sep 2017, Published online: 12 Oct 2017

ABSTRACT

Amyloids represent protein fibrils that have highly ordered structure with unique physical and chemical properties. Amyloids have long been considered lethal pathogens that cause dozens of incurable diseases in humans and animals. Recent data show that amyloids may not only possess pathogenic properties but are also implicated in the essential biological processes in a variety of prokaryotes and eukaryotes. Functional amyloids have been identified in archaea, bacteria, fungi, and animals, including humans. Plants are one of the most poorly studied groups of organisms in the field of amyloid biology. Although amyloid properties have not been shown under native conditions for any plant protein, studies demonstrating amyloid properties for a set of plant proteins in vitro or in heterologous systems in vivo have been published in recent years. In this review, we systematize the data on the amyloidogenic proteins of plants and their functions and discuss the perspectives of identifying novel amyloids using bioinformatic and proteomic approaches.

INTRODUCTION

Amyloids are protein fibrils with a characteristic structure called “cross-β”. This structure results from the formation of intermolecular β-sheets perpendicular to the axis of the amyloid fibrilsCitation1 and is detected using two-dimensional X-ray diffraction.Citation2 The stacking of monomers in the fibrils results from the formation of numerous hydrogen bonds between neighboring β-chains.Citation3 Such spatial organization makes amyloids one of the most stable biogenic particles and endows these macromolecules with unique physico-chemical properties: amyloids resist treatment with different detergents (like SDS and sarkosyl), proteinases, high temperatures, and several acids,Citation2,4-7 and can persist in the environment for years.Citation8 In addition, the unique spatial structure of amyloids can be specifically detected with several compounds. The binding of amyloids with Congo red diazo dye leads to apple-green birefringence in the polarized light,Citation9 while the interaction of amyloids with the fluorescent dye Thioflavin-T enhances the emission of its fluorescence.Citation10,11

Amyloids have long been considered as pathogens for humans and animals causing incurable diseases called amyloidoses. The first pathanatomic descriptions of amyloidoses in the liver and spleen were obtained in the XVII or XVIII centuries, while the protein nature of pathological amyloid deposits was revealed only in 1858.Citation12 In 1854, Rudolf Virchow proposed the term “amyloid” (starch-like),Citation13 since amyloid deposits were stained a blue color using iodine similarly to starch granules. It has since been confirmed that the blue staining reflects the complex organization of amyloid deposits comprising, in addition to protein fibrils, proteoglycans and glycosaminoglycans.Citation14 To date, more than 30 proteins forming pathological or pathology-associated amyloids have been identified in humans.Citation15 Infectious neurodegenerative amyloidoses represent a special group of diseases caused by the amyloid state of prion (an acronym from proteinaceous infectious particle) protein (PrP), which was called PrPSc (PrP Scrapie, from scrapie disease of sheep and goats).Citation7,16 Presently, the term “prion” is attributed to all infectious amyloids. Nevertheless, there are prions that are unlikely to form amyloids.Citation17,18 In a broader sense, prions are proteins that persist under the same conditions in two or more conformations, of which at least one conformation possesses infectious properties.Citation19 Thus, amyloids and prions represent partially overlapping sets.

Since 2000, the paradigm of amyloids as pathogens has rapidly shifted. The formation of the amyloid structure not only leads to lethal diseases but is also necessary for essential biological functions. In different organisms, from archaea and bacteria to humans, more than 20 functional amyloids have been described, and their number is constantly growing.Citation20-22 In prokaryotes, at least eight groups of proteins capable of forming functional amyloids were identified. These amyloids participate in the biofilm formation,Citation23 overcoming the surface tension,Citation24 storage of toxins,Citation25 formation of pores in phagolysosome membraneCitation26 or hypersensitive response activation in plants.Citation27 In animals, amyloids are involved in long-term memory formation,Citation28,29 melanin polymerization,Citation30 hormone storage,Citation31 tooth enamel biomineralization,Citation32 programmed necrosis regulationCitation33 and the matrix formation in spermatozoid acrosome.Citation34

Prions of ascomycetes represent a specific group of infectious amyloids. There are approximately ten prions in yeast Saccharomyces cerevisiae.Citation35 In contrast to non-infectious amyloids, the aggregates of prions are constantly fragmented by chaperone proteins,Citation36,37 providing the infectious properties and efficient transmission of prions during cell division.Citation38,39 The prionization of proteins may lead to heritable changes in their functional activities that cause different phenotypic manifestations. This phenomenon is referred to as protein inheritance,Citation40,41 i.e., an inheritance that is not determined by changes in the primary structure of genes, but rather the conformation of particular proteins. Most yeast prions have no significant effect on viability; however, some of them may be harmful under certain conditions,Citation42 and in contrast, some prions may increase cell survival.Citation43,44

Thus, numerous amyloids involved in pathogenesis or implicated in a variety of functions have been identified in different organisms. At the same time, plants remain one of the most poorly studied group of organisms in the field of amyloid biology reflecting, on the one hand, the high complexity of working with plants as objects of research, and, on the other hand, the lack of methods for detecting amyloids at the proteomic level. In this review, we systematize the data on amyloidogenic proteins of plants, analyze their potential biological functions, and discuss the perspectives for the identification of novel amyloids in plants.

I. Amyloids Involved in the Adhesion of Green Algae

Life forms of green algae are diverse, and many of these organisms form biofilms attached to different surfaces. The adhesion of the spores of green algae includes two stages: initial and constant adhesion.Citation45 At the initial stage, preceded by a complex mechanism involving the recognition of a proper substrate, spores attach to the surface using a sticky secretion present on their shells. Subsequently, the spores actively produce special gel-like extracellular polymeric substances (EPS) comprising different proteins, glycoproteins, and proteoglycans. EPS bind Thioflavin-T and exhibit apple-green birefringence upon staining with Congo red.Citation46,47 Moreover, Raman spectroscopy revealed EPS peaks similar to those observed for known amyloids.Citation46 These data suggest that the EPS of green algae, including micro- (Coccomyxa sp. or Glaphyrella trebouxiodes)Citation46 and macrophytes (Prasiola linearis),Citation47 may contain protein (or proteins) in an amyloid state. These proteins might be the main determinants of the high mechanical resistance of EPS. Amyloids are involved in the biofilm formation and adhesion of different species of bacteria.Citation22,23 Thus, adhesive proteins represent an example of cross-kingdom conservation of biological functions of amyloids. Nevertheless, the particular amyloid-forming proteins of EPS were not identified, and a complete understanding of the role of amyloid formation for green algae adhesion requires further investigation.

Notably, amyloids are important structural elements for different biological substances. The egg envelopes (chorions) of “annual fish” Austrofundulus limnaeus, which lives in the annually drying reservoirs of South America, contain amyloid proteins. The amount of amyloids in these envelopes increases with dehydration, which increases the survival of embryos.Citation48 The matrix of mice spermatozoid acrosome contains a range of proteins forming detergent-resistant aggregates with amyloid properties.Citation49 The chorion of silk moth eggs bears at least two classes of amyloid-forming proteins.Citation50 Thus, similar to the aforementioned structures, the formation of EPS might implicate ensembles of various amyloid-forming proteins likely to co-aggregate as a result of their structural similarity.

II. Prion-like Protein Luminidependens of Arabidopsis thaliana

Flowering time in Arabidopsis thaliana is controlled by a complex molecular mechanism involving several cascades of transcriptional factors,Citation51 one of which is so-called Autonomous Flowering Pathway (AFP). This regulatory pathway is independent of the photoperiod and vernalization, i.e., induction of flowering as a result of a prolonged influence of low, typically positive temperatures.Citation52

Recently, a paper by S. Chakrabortee and co-authors, from the laboratory of S. Lindquist demonstrated that Luminidependens (LD), an AFP protein in A. thaliana, has prion properties in the heterologous system of the yeast Saccharomyces cerevisiae.Citation53 Most known yeast prion proteins are rich in asparagine (N) and glutamine (Q).Citation54,55 LD was predicted as a potentially prion-forming protein using a recently developed bioinformatic algorithm based on a hidden Markov model,Citation56 as the prion-forming region of A. thaliana protein was closely similar to those of yeast prion proteins.Citation53 Notably, the fraction of proteins acting as flowering regulators was statistically significantly increased in a set of approximately 500 A. thaliana proteins detected using this algorithm.Citation53 Further experimental analysis demonstrated that LD and two other AFP proteins, Flowering Locus PA (FPA) and Flowering Locus CA (FCA), form detergent-resistant oligomers in yeast cells. Moreover, LD fused with the reporter sequences demonstrated properties typical for yeast prions: dominance, cytoplasmic infectivity, and dependence on the level of Hsp70 chaperone production.Citation53

Thus, LD forms a chimeric prion in yeast cells. Does this finding suggest that LD has prion properties in A. thaliana cells? Indeed, drawing this conclusion would be at least premature. The fact that LD forms detergent-resistant oligomers in a heterologous system at an increased level of production confirms neither amyloid fibril formation nor prion-like properties under native conditions in A. thaliana. Nevertheless, the role of epigenetic regulation in flowering induction in A. thaliana has been demonstrated in a number of studies. Vernalization results in the silencing of the FLOWERING LOCUS C (FLC), the product of which is a protein that represses flowering.Citation52 The FLC gene is also a target for LD with a nuclear localizationCitation57 and inhibits the expression of FLC in meristems via histone modificationCitation58 and the negative regulation of the SUF4 activator of FLC expression.Citation59 Prionization typically entails the functional inactivation of prion-forming protein;Citation60 therefore, according to the hypothesis of Y. Chernoff, the most likely phenotype of LD prionization would be the delay of the flowering.Citation61 Notably, temperature is not only a factor controlling vernalization in plants but also affects both the inductionCitation62 and stability of prions.Citation63 Thus, the influence of temperature on flowering could also be modulated through changes in the induction frequency or the effectiveness of LD prion propagation.

III. Plant Proteins with Amyloid Properties in Vitro

Several plant proteins possess amyloid properties in vitro. The monellin protein of the tropical fruit Dioscoreophyllum cumminsii irreversibly denatures when boiled at 85°C in acidic (pH 2.5) buffer. The consequent addition of 100 mM sodium chloride induces the formation of monellin fibrils that bind Congo red.Citation64 Unfortunately, birefringence upon binding of Congo red with monellin fibrils was not analyzed, therefore it is impossible to draw a conclusion concerning the amyloid nature of this protein, even in vitro.

Maize transglutaminase (TGZ) forms insoluble inclusions when overproduced under the control of the strong psbA promoter in vivo in the transplastomic tobacco plants.Citation65 These inclusions contain fibrillar aggregates of TGZ resistant to SDS treatment.Citation65 In vitro, the TGZ protein forms detergent-resistant aggregates of a fibrillar structure showing birefringence upon binding with Congo red. The fibrils of the short C-terminal (aa 466–477) region of TGZ have similar properties in vitro.Citation65 Therefore, maize TGZ forms amyloid-like fibrils in vitro and in the transplastomic tobacco plants.

Prohevein protein is the precursor of hevein, a key component of Hevea brasiliensis latex, and has an evolutionarily conserved C-terminal region, which, in contrast to the full-length protein, forms fibrils in vitro.Citation66 These fibrils exhibit a pattern typical for amyloids in two-dimensional X-ray diffraction and apple-green birefringence upon binding with Congo red.Citation66 Notably, the C-terminal region of progevein is also present in the latex of the hevea and exhibits agglutination.Citation67 Thus, the amyloid properties of the C-terminal region of progevein could enhance its stability in vivo and the agglutination of pathogenic microorganisms and fungi, facilitating their destruction by the enzymes of the lutoid fraction of latex.Citation66

Several defense peptides of plants were recently demonstrated as exhibiting amyloid properties in vitro. For example, the antimicrobial peptide Cn-AMP2 from coconut (Cocos nucifera) liquid endosperm contains bioinformatically predicted amyloidogenic regions and demonstrates amyloid properties in vitro, including the formation of fibrils detectable using electronic microscopy and the enhancement of the fluorescence of Tioflavin-T dye.Citation68 Another example is defensins, which are short plant proteins involved in protection from various pathogens. The RsAFP-19 peptide is a C-terminal fragment (19 residues) of RsAFP1 and RsAFP2 defensins of the radish Raphanus sativus. This peptide exhibits fungicidal activity. Freeze-thaw cycles induce RsAFP-19 aggregation in vitro. These aggregates have a fibrillar structure and exhibit a cross-β pattern in X-ray diffraction typical for amyloids.Citation69 It is not known whether the full-length defensins RsAFP1 and RsAFP2 form amyloid aggregates in vivo, but the fungicidal activity of RsAFP-19 is negatively correlated with the level of its aggregation.Citation69 A similar effect has been reported for the amyloids of bacterial microcin E492.Citation25 Thus, one of the potential biological roles of amyloid formation by plant defense proteins might be the sequestration of the toxic intermediates of these proteins into the functionally inactive fibrils.Citation25,70

Taken together, the studies conducted to date show that several plant proteins or their fragments have amyloid properties in vitro or in heterologous systems (). Whether these molecules have amyloid properties in the native conditions remains unclear and should be analyzed in additional studies. Further, is it likely that plants have other amyloid-forming proteins?

TABLE 1. Amyloidogenic proteins in plants.

IV. Perspectives for the Identification of Novel Amyloids and Prions in Plants

The identification of novel prions and amyloids remains arduous and time-consuming. Significant progress in this field of biology can be provided using novel bioinformatic and proteomic methods. The methodology of proteomic analysis of amyloids is currently at the very dawn of its development. The first attempts to apply proteomics for studying amyloidsCitation71-73 were associated with the identification of proteins sequestered by pathological amyloids that form large deposits and can be isolated from fixed tissue by laser capture microdissection (LCM).Citation74 A proteome-wide method for the identification of candidates for novel amyloid-forming proteins, called TAPI (Technique for Amyloid Purification and Identification), was proposed in 2013.Citation75 TAPI uses polyacrylamide gel electrophoresis for the ultrafiltration of detergent-treated amyloid-rich protein fractions, followed by HPLC combined with mass spectrometry to identify candidate proteins.Citation75 In 2014, another method called PSIA (Proteomic Screening and Identification of Amyloids) was developed.Citation76 This method uses differential ultracentrifugation combined with treatment using ionic detergentsCitation77 to obtain protein fractions rich in amyloid proteins. These fractions are solubilized, and the proteins (peptides) are separated either by two-dimensional gel electrophoresisCitation76 or HPLCCitation78,79 coupled with mass spectrometry, which provides high resolution sufficient to identify the most minor amyloid-forming proteins.Citation79 Nevertheless, to date, proteomic methods have not been applied for the identification of amyloid proteins in plant species. Is it possible to assess the amyloid properties of plant proteins by other methods?

The primary structure of proteins is the key determinant of their amyloidogenic properties. In 1997, the polyglutamine tracts capable of forming amyloids involved in the development of neurodegenerative diseases in humans were described.Citation80 Subsequently, it was noted that the common feature of different structural proteins of infectious yeast amyloids (prions) is the presence of regions rich in Q or N residues.Citation81 Homopeptides formed by several other amino acids, particularly E, also form amyloids.Citation82 Such amyloidogenic regions (type I amyloidogenic regions) are rich in one or similar (such as Q and N) residues. To identify these regions, several bioinformatic algorithms were developed.Citation54,83 Notably, the relative content of the biased residues is more important for the formation of type I amyloidogenic regions than the positions of the particular residues.Citation84

QN-rich proteins are widely spread throughout plant proteomes. Thus, in Arabidopsis thaliana, approximately 200 such proteins were detected,Citation54 three of which, the transcriptional regulators LD, FPA, and FCA discussed above (see Section II, ), showed some properties of amyloids.Citation53 Enrichment in Q and N is a characteristic feature of transcription factors, and of other DNA- and RNA-binding proteins of various organisms.Citation54 Moreover, polyasparagine and polyglutamine tracts display transcriptional activity.Citation85,86 Transcription factors are abundant in the actively dividing cells of plant meristems. These tissues are promising for identifying novel plant amyloids likely to be involved in regulatory processes and having prion-like properties.

The enrichment in Q and E is a common feature of many plant seed storage proteins. For example, seed storage proteins of maize (Zea mays), rye (Secale sereale), wheat (Triticum aestivum), oat (Avena sativa), and other important crops are rich in Q and P,Citation87 and the storage proteins of legumes are rich in E and D.Citation88 The ability to form fibrils, which are widely used in biotechnology, is well known for maize prolamins (zeins).Citation89 In addition, studies on the structure of zein fibrils did not demonstrate characteristic signals in X-ray diffraction inherent in amyloids.Citation90 The fractions enriched with seed storage proteins of pea (Pisum sativum)Citation91 and soy (Glycine max)Citation92 were subjected to the limited proteolysis at a temperature of more than 80°C and pH = 2, and the resulting peptides formed fibrils with enhanced fluorescence of Thioflavin-T dye, suggesting an amyloid structure.Citation92 Similar results were obtained with peptide mixtures formed after trypsin digestion of the wheat gliadin and gluten storage proteins.Citation93 Unfortunately, the amyloid properties of the full-length seed storage proteins were never tested, and specific peptides capable of forming amyloids were not identified.Citation91-93 Notably, a significant portion of proteins contain bioinformatically predicted amyloidogenic peptides, which are capable of forming amyloid-like fibrils at high concentrations and under special conditions,Citation94 but this fact does not indicate that the corresponding full-length proteins have amyloid properties.

Notably, natural dehydration occurs during maturation of plant seeds. This process is accompanied by the compaction of genetic material, a decrease in metabolic rate, and a change in the structure of proteins.Citation95,96 This process is similar to the dehydration of the eggs of the “annual fish” Austrofundulus limnaeus leading to the amyloidogenesis of the proteins in its envelopes, which increases the survival of embryos.Citation48 It can be assumed that seed storage proteins during dehydration also form amyloids, which increase their stability, and subsequently, under favorable conditions, these proteins revert to a monomeric or oligomeric state that can easily be metabolized by the growing embryo.

QN-rich proteins represent approximately one-third of the currently known amyloids.Citation97 For other amyloid-forming proteins, strict patterns of the primary structure have not been identified. However, various amino acids have different amyloidogenic propensities. For example, hydrophobic aromatic (W, F) and some aliphatic monoamino monocarboxylic (I, V, L) amino acids have the highest amyloidogenic propensity (i.e., the ability to induce amyloid formation).Citation98 The amyloidogenic regions formed by these amino acids (type II amyloidogenic regions), unlike type I regions, do not have one prevalent amino acid. The specific position of the corresponding amino acids is of particular importance for the formation of type II regions. Removing or changing the position of only one amino acid in such amyloidogenic regions can lead to a complete loss of amyloid properties.Citation99 Approximately ten different bioinformatic algorithms have been developed for the prediction of type II amyloidogenic regions.Citation98

Defense proteins and peptides of plants are structurally and functionally heterogeneous groups that are not characterized by the enrichment of Q and N; however, many of these molecules are hydrophobicCitation100 and thus may contain type II amyloidogenic regions. Plants produce a wide range of various defense peptides and proteins.Citation101,102 As previously discussed, the amyloid properties for some of these molecules are shown, but are still not entirely clear (, ). Since amyloids represent one of the most stable variants of the quaternary structure of a protein, we propose that adopting an amyloid state by defense proteins could not only promote the inactivation of toxic intermediates but also increases the effectiveness of these proteins against various pathogens.

FIGURE 1. Location of potentially amyloidogenic proteins in plants. A schematic illustration of the plant is shown. The names of proteins or peptides, whose amyloid properties were partially characterized in vivo (green) and in vitro (orange), and proteins, whose fragments have amyloidogenic properties (black), are indicated. The data on these proteins and peptides are summarized in .

FIGURE 1. Location of potentially amyloidogenic proteins in plants. A schematic illustration of the plant is shown. The names of proteins or peptides, whose amyloid properties were partially characterized in vivo (green) and in vitro (orange), and proteins, whose fragments have amyloidogenic properties (black), are indicated. The data on these proteins and peptides are summarized in Table 1.

CONCLUSION

To date, amyloid properties were shown for several plant proteins or their fragments in vitro or in heterologous systems in vivo. However, no plant proteins were shown to form amyloids under native conditions. Based on the data obtained in previous studies (, ), we distinguished four groups of plant proteins promising for the identification of novel amyloids: (i) QN-rich proteins, particularly those capable of binding nucleic acids; (ii) defense proteins and peptides containing hydrophobic regions; (iii) seed storage proteins containing Q- and E-rich regions; and (iv) proteins involved in the adhesion of algal cells to the surfaces. Based on the methodology developed to date, we can propose two main strategies to reveal candidates for functional amyloids in the proteomes of plants: (i) bioinformatic prediction of potentially amyloidogenic proteins and (ii) proteomic screenings of proteins resistant to treatment with ionic detergents. In both cases, the amyloid properties of candidate proteins should be verified by analyzing their ability to form fibrils and adopt cross-β structure. Further studies on the bioinformatic prediction and experimental identification of amyloid proteins in the proteomes of different species are required to elucidate the biological roles and functions of plant amyloids.

ABBREVIATIONS

AFP=

Autonomous Flowering Pathway

EPS=

Extracellular Polymeric Substances

HPLC=

High Performance Liquid Chromatography

LPS=

Lowest Probability Subsequences

PSIA=

Proteomic Screening and Identification of Amyloids

SDS=

Sodium Dodecyl Sulfate

Sarkosyl=

Sodium lauroyl sarcosinate

SARP=

Sequence Analysis Based on the Ranking of Probabilities

TAPI=

Technique for Amyloid Purification and Identification; The standard single-letter amino acid code is used

DISCLOSURE OF POTENTIAL CONFLICTS OF INTEREST

Authors declare no potential conflicts of interest.

FUNDING

This work was financially supported by a grant from the Russian Science Foundation (Project No 17-16-01100).

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