Pulmonary Fibrosis Effect of Ammonium Perchlorate Exposure in Rabbit

ABSTRACT

Ammonium perchlorate (AP) is a kind of inorganic chemical. To evaluate the potential pulmonary fibrosis caused by occupational exposure to this compound, 25 male rabbits were randomly allocated into 5 groups to receive AP or bleomycin or saline by intratracheal injection. All rabbits were sacrificed and lung tissues were removed to prepare hematoxylin and eosin (HE) staining for microscope observation and to perform electron microscopy examine. In microscope observation, AP-instilled lung tissue showed inflammatory infiltrates, alveolar collapse, subpleural thickening, and lymphocyte proliferation. Electron microscopy examination of lung tissue showed massive fibroblast accumulation, collagen fiber hyperplasia, and dense collagen deposition. The histopathological changes were considered as effective indicator linking to the development of pulmonary fibrosis. These results demonstrated that chronic instillation of AP can induce pulmonary fibrosis. And these results revealed that AP has pulmonary toxic effect.

KEYWORDS:

• ammonium perchlorate
• collagen
• pulmonary fibrosis

Acknowledgments

This study was funded by the National Natural Science Foundation of China (grant no. 30471441), whose support of this work is gratefully appreciated.