Effect of Tangeritin Against Cyclophosphamide-Induced Toxicity in the Larvae of Transgenic Drosophila melanogaster (hsp70-lac Z) Bg⁹

ABSTRACT

Tangeritin is a pentamethoxy flavone found in tangerine and other citrus peels. The present study was conducted to study the effect of various doses of tangeritin on the toxic effects induced by cyclophosphamide (CP) in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ)Bg⁹. Commercially available tangeritin and CP were used. CP was used alone at 20 µM of food concentration and with 20, 40, and 80 µM of tangeritin. The exposure of the third instar larvae to 20µM of cyclophosphamide alone results in significant hsp 70 expression along with tissue, DNA, and oxidative damage. The exposure of the third instar larvae to 20 µM of CP along with 20, 40, and 80 µM of tangeritin results in a dose-dependent decrease not only in hsp 70 expression but also in tissue, DNA, and oxidative damage. The results of the present study suggest that tangeritin has a protective role against the toxic effects of cyclophosphamide in the third instar larvae of transgenic Drosophila melanogaster (hsp 70-lac Z)Bg⁹.

KEYWORDS:

• Cyclophosphamide
• Drosophila melanogaster (hsp70-lac Z)Bg⁹
• tangeritin
• toxicity

Acknowledgments

The authors are thankful to the Chairman, Department of Zoology, AMU Aligarh for providing laboratory facilities.

Declaration of interest

The authors declare no conflicts of interest. The authors alone are responsible for the content and writing of the article.

Additional information

Notes on contributors

Ambreen Fatima

Mrs. Ambreen Fatima (M.Sc.), Mrs. Saba Khanam (M.Sc.), Ms. Falaq Naz (M.Phil), and Mr. Rahul (M.Phil) are doctoral students.

Saba Khanam

Mrs. Ambreen Fatima (M.Sc.), Mrs. Saba Khanam (M.Sc.), Ms. Falaq Naz (M.Phil), and Mr. Rahul (M.Phil) are doctoral students.

Smita Jyoti

Dr. Smita Jyoti (Ph.D.) is currently working as research assistant in the ICMR, New Delhi funded research project.

Falaq Naz

Mrs. Ambreen Fatima (M.Sc.), Mrs. Saba Khanam (M.Sc.), Ms. Falaq Naz (M.Phil), and Mr. Rahul (M.Phil) are doctoral students.

  Rahul

Mrs. Ambreen Fatima (M.Sc.), Mrs. Saba Khanam (M.Sc.), Ms. Falaq Naz (M.Phil), and Mr. Rahul (M.Phil) are doctoral students.

Tanveer Beg

Dr. Tanveer Beg (Ph.D.) is working as an assistant professor, Department of Biology, Jazan University, Jazan, KSA.

Yasir Hasan Siddique

Dr. Yasir Hasan Siddique (Ph.D.) is working as an assistant professor in Department of Zoology, AMU, Aligarh.