ABSTRACT
Tangeritin is a pentamethoxy flavone found in tangerine and other citrus peels. The present study was conducted to study the effect of various doses of tangeritin on the toxic effects induced by cyclophosphamide (CP) in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ)Bg9. Commercially available tangeritin and CP were used. CP was used alone at 20 µM of food concentration and with 20, 40, and 80 µM of tangeritin. The exposure of the third instar larvae to 20µM of cyclophosphamide alone results in significant hsp 70 expression along with tissue, DNA, and oxidative damage. The exposure of the third instar larvae to 20 µM of CP along with 20, 40, and 80 µM of tangeritin results in a dose-dependent decrease not only in hsp 70 expression but also in tissue, DNA, and oxidative damage. The results of the present study suggest that tangeritin has a protective role against the toxic effects of cyclophosphamide in the third instar larvae of transgenic Drosophila melanogaster (hsp 70-lac Z)Bg9.
Acknowledgments
The authors are thankful to the Chairman, Department of Zoology, AMU Aligarh for providing laboratory facilities.
Declaration of interest
The authors declare no conflicts of interest. The authors alone are responsible for the content and writing of the article.
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Notes on contributors
Ambreen Fatima
Mrs. Ambreen Fatima (M.Sc.), Mrs. Saba Khanam (M.Sc.), Ms. Falaq Naz (M.Phil), and Mr. Rahul (M.Phil) are doctoral students.
Saba Khanam
Mrs. Ambreen Fatima (M.Sc.), Mrs. Saba Khanam (M.Sc.), Ms. Falaq Naz (M.Phil), and Mr. Rahul (M.Phil) are doctoral students.
Smita Jyoti
Dr. Smita Jyoti (Ph.D.) is currently working as research assistant in the ICMR, New Delhi funded research project.
Falaq Naz
Mrs. Ambreen Fatima (M.Sc.), Mrs. Saba Khanam (M.Sc.), Ms. Falaq Naz (M.Phil), and Mr. Rahul (M.Phil) are doctoral students.
Rahul
Mrs. Ambreen Fatima (M.Sc.), Mrs. Saba Khanam (M.Sc.), Ms. Falaq Naz (M.Phil), and Mr. Rahul (M.Phil) are doctoral students.
Tanveer Beg
Dr. Tanveer Beg (Ph.D.) is working as an assistant professor, Department of Biology, Jazan University, Jazan, KSA.
Yasir Hasan Siddique
Dr. Yasir Hasan Siddique (Ph.D.) is working as an assistant professor in Department of Zoology, AMU, Aligarh.