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Abstract
The aflatoxins content of 140 cotton seed samples were determined using high-performance liquid chromatography. Samples were obtained from wholesalers in Iran between May 2010 and June 2011. Aflatoxin B1 gave the highest incidence of contamination and was found in 129 of the 139 samples. The highest concentration of aflatoxin was 14.4 ng g−1. Thirteen cotton seed samples (9.35%) were above one of the regulatory limits of the European Union (5 ng g−1), but no sample was above the highest EU limit and the safety limit recommended by the FDA (20 ng g−1) and regulatory limits of Iran (50 ng g−1) for total aflatoxin.