Corrigendum

This article refers to:

Pregnancy-specific glycoprotein expression in normal gastrointestinal tract and in tumors detected with novel monoclonal antibodies

Article Title: Pregnancy-specific glycoprotein expression in normal gastrointestinal tract and in tumours detected with novel monoclonal antibodies

Author(s): Aileen Houston, John M. Williams, Tihana Lenac Rovis, Daniel K. Shanley, Ronan T. O'Riordan, Patrick A. Kiely, Melanie Ball, Orla P. Barry, Jacquie Kelly, Aine Fanning, John MacSharry, Ofer Mandelboim, Bernhard B. Singer, Stipan Jonjic, and Tom Moore.

Journal: mAbs

Bibliometrics: Published in Volume 8, pp. 491–500.

DOI: 10.1080/19420862.2015.1134410

Publisher: Taylor & Francis

In the above article, published in issue 8(03) of mAbs, the Author inadvertently omitted details of construction of some PSG expression vectors. All vectors comprise the relevant PSG open reading frame (ORF) subcloned into the pTT3 expression vector in-frame with a carboxy terminus V5/His tag obtained from the pBlueBac4.5-V5/His vector. Vectors expressing full-length PSG1 and PSG1 deletion variants were described previously. (Shanley et al., 2013; Houston et al., 2016) In most cases, other PSG ORFs were obtained from IMAGE clones (http://www.imageconsortium.org/) by PCR and were directionally subcloned into pTT3 using PCR primers containing EcoRI and HindIII restriction sites. Where ORF coding sequences had internal EcoRI or HindIII sites, or for other reasons, additional steps included site-directed mutagenesis or use of intermediate cloning vectors, prior to subcloning into pTT3 (details available on request). The origin and/or IMAGE ID of PSG cDNA clones were as follows: PSG2, SC107858; PSG3, 4043628; PSG4, 4768860; PSG5, 4043912; PSG6, gift from W. Zimmermann (NM_002782.4); PSG7, 4605177; PSG8, 40147510; PSG9, gift from W. Zimmermann (NM_002784); PSG11, RC214328.