Simplified RP-HPLC method for multi-residue analysis of abamectin, emamectin benzoate and ivermectin in rice

Abstract

A rapid, reliable and sensitive reverse-phase high-performance liquid chromatography method with fluorescence detection (RP-FLD-HPLC) was developed and validated for simultaneous analysis of the abamectin (ABA), emamectin (EMA) benzoate and ivermectin (IVM) residues in rice. After extraction with acetonitrile/water (2 : 1) with sonication, the avermectin (AVMs) residues were directly derivatised by N-methylimidazole (N-NMIM) and trifluoroacetic anhydride (TFAA) and then analysed on RP-FLD-HPLC. A good linear relationship (r ²> 0.99) was obtained for three AVMs ranging from 0.01 to 5 µg ml⁻¹, i.e. 0.01–5.0 µg g⁻¹ in rice matrix. The limit of detection (LOD) and the limit of quantification (LOQ) were between 0.001 and 0.002 µg g⁻¹ and between 0.004 and 0.006 µg g⁻¹, respectively. Recoveries were from 81.9% to 105.4% and precision less than 12.4%. The proposed method was successfully applied to routine analysis of the AVMs residues in rice.

Keywords:

• abamectin (ABA)
• emamectin (EMA) benzoate
• ivermectin (IVM)
• residue

Acknowledgements

This study was supported by the key project of developing agriculture (No. 2003-10-6) from the Shanghai Agriculture Committee and the standard-technology programme (No. 08GWQ068) from the Shanghai Science & Technology Committee.