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Food Additives & Contaminants: Part A Volume 28, 2011 - Issue 12
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Original Articles

Cross-reactivity of some commercially available deoxynivalenol (DON) and zearalenone (ZEN) immunoaffinity columns to DON- and ZEN-conjugated forms and metabolites

A. Veršilovskis Veterinary and Agrochemical Research Centre (CODA-CERVA), Operational Direction Chemical Safety of the Food Chain, Unit of Toxins and Natural Compounds, Leuvensesteenweg 17, B-3080 Tervuren, Belgium; Department of Bio-Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, B-9000 Gent, Belgium; Institute of Food Safety, Animal Health and Environment ‘BIOR’, Lejupesiela 3, B-1076 Riga, LatviaCorrespondence[email protected] [email protected]
,
B. Huybrecht Veterinary and Agrochemical Research Centre (CODA-CERVA), Operational Direction Chemical Safety of the Food Chain, Unit of Toxins and Natural Compounds, Leuvensesteenweg 17, B-3080 Tervuren, Belgium
,
E.K. Tangni Veterinary and Agrochemical Research Centre (CODA-CERVA), Operational Direction Chemical Safety of the Food Chain, Unit of Toxins and Natural Compounds, Leuvensesteenweg 17, B-3080 Tervuren, Belgium
,
L. Pussemier Veterinary and Agrochemical Research Centre (CODA-CERVA), Operational Direction Chemical Safety of the Food Chain, Unit of Toxins and Natural Compounds, Leuvensesteenweg 17, B-3080 Tervuren, Belgium
,
S. De Saeger Department of Bio-Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, B-9000 Gent, Belgium
&
A. Callebaut Veterinary and Agrochemical Research Centre (CODA-CERVA), Operational Direction Chemical Safety of the Food Chain, Unit of Toxins and Natural Compounds, Leuvensesteenweg 17, B-3080 Tervuren, Belgium
Pages 1687-1693 | Received 27 Mar 2011, Accepted 03 Jul 2011, Published online: 01 Aug 2011
 
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Abstract

Seven commercially available deoxynivalenol (DON) and zearalenone (ZEN) immunoaffinity columns (IACs) were tested for cross-reactivity to conjugated forms (3-acetyl-deoxynivalenol, 15-acetyl-deoxynivalenol, DON-3-glucoside, DON-3-glucuronide, ZEN-glucosides, ZEN-glucuronide) and metabolites (de-epoxydeoxynivalenol, α-zearalenol, β-zearalenol) and nivalenol (NIV), using a semi-quantitative multi-mycotoxin ultra-performance liquid chromatography-tandem mass spectrometry method. The DON IACs showed cross-reactivity for nearly all DON derivatives tested. The ZEN IACs showed limited cross-reactivity to some of the ZEN derivatives. The IACs were evaluated for their potential use as sample clean-up for mycotoxins in serum.

Acknowledgements

The authors wish to thank Neogen Corporation (Neogen Co., Ayr, UK) for providing the immunoaffinity columns.

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