Analysis of fumonisin contamination and the presence of Fusarium in wheat with kernel black point disease in the United States

Abstract

The ability of the fungus Fusarium proliferatum to cause kernel black point disease in wheat was previously established, but natural contamination of black point wheat with both F. proliferatum and fumonisin mycotoxins has not been studied in the United States. Low levels of fumonisins were detected in nine of 43 wheat samples with kernel black point disease that were obtained from across the United States. A subset of samples was contaminated with F. proliferatum as well as with F. fujikuroi, F. nygamai, F. thapsinum and F. verticillioides, species closely related to F. proliferatum and morphologically similar to it in that they produce chains of asexual spores, or conidia. Nevertheless, of conidial chain-forming fusaria isolated from symptomatic wheat, F. proliferatum dominated. In greenhouse tests, isolates of F. proliferatum and the other species recovered from wheat samples were able to cause symptoms of kernel black point and, in some cases, low levels of fumonisin contamination of wheat. These data add to the understanding of the risk of fumonisin contamination of wheat and the potential for Fusarium species to cause kernel black point disease and fumonisin contamination of wheat. Further, the results of this study indicate that while US-grown wheat can sporadically be contaminated by fumonisins, the natural contamination levels seem to be low. The observations made provide evidence that fumonisins are not likely to be a factor contributing to the ability of Fusarium to cause kernel black point disease.

Keywords:

• LC/MS
• microbiology
• survey
• mycotoxins – fumonisins
• cereals and grain

Acknowledgements

The authors gratefully acknowledge G.C. Bergstrom, J.D. Busman, F.E. Dowell, A. Hollister, A.J. Lukaszewski and D.G. Schmale for providing wheat samples. They also acknowledge D. Palmquist for assistance in the statistical treatment of the data. The authors also thank Stephanie Folmar, Marcie Moore, Nathan Deppe, Ashley Cranford, Nathaniel Puent and Deborah Shane for technical assistance. Mention of trade names or commercial products herein is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture (USDA). The USDA is an equal opportunity provider and employer.