Susceptibility of different mice strains to okadaic acid, a diarrhetic shellfish poisoning toxin

Abstract

The mouse bioassay is widely used to detect diarrhetic shellfish poisoning (DSP) toxins. To the best of our knowledge, however, there have been no reports specifically on strain differences in susceptibility to DSP toxins. In this study, we investigated the susceptibility of different mice strains to okadaic acid (OA), one of the representative DSP toxins. A lethal dose of OA was injected intraperitoneally (i.p.) into mice. The mice were observed until 24 h after injection. Five inbred strains (A/J, BALB/c, C3H/He, C57BL/6, and DBA/2) and two non-inbred strains (ddY, and ICR) of mice were compared. All the mice were male, weighed 16–20 g, and were 4–5 weeks old. The lethality was 90–100% in the A/J, BALB/c, ddY, and ICR strains, 70–80% in the C3H/He and C57BL/6 strains, and 40% in DBA/2 strain. Survival analysis showed that the BALB/c, C57BL/6, ddY, and ICR strains died earlier and the A/J, C3H/He and DBA/2 strains survived longer. These results indicate that significant differences may exist in the susceptibility of mice strains to OA.

Keywords:

• bioassay
• toxicology, in vivo
• phycotoxins, DSP
• seafood

Acknowledgements

This study was supported by Health and Labour Sciences Research Grants, Research on Food Safety from the Ministry of Health, Labour and Welfare of Japan.