![Sample our Bioscience journals, sign in here to start your access, Latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5925/TOC-Subject-Sample-2021-Bioscience.jpg"})
Abstract
In this paper, a method for the detection of norbixin and tartrazine in sugar by means of resonance Raman spectroscopy is presented. The extraction was done in four steps using methanol and the measurements were performed in aqueous solution. The excitation wavelength was 514 nm for norbixin and 488 nm for tartrazine samples. The characteristic resonance Raman signals of the dyes were fitted by different functions. Depending on the R 2 values of the different fits, each spectrum was classified as positive or negative response. A detection limit of 250 ng g−1 for norbixin and 989 ng g−1 for tartrazine in solid sugar samples could be reached by logistic regression.
Acknowledgements
We gratefully acknowledge the Free State of Thuringia and the European Union (EFRE) for financial support under support code 2008FE9112 (BioOptiSens). Furthermore, we thank our technical staff Anna Schmidt, Gisela Persch and Petra Weiß for the preparation of the samples.