Fusarium infection of maize and maize-based products and exposure of a rural population to fumonisin B₁ in Limpopo Province, South Africa

Abstract

Fusarium species (spp.) and fumonisin B₁ (FB₁) contaminations were monitored in maize and porridge consumed by a rural population of Limpopo Province, South Africa. Faecal samples were also analysed for FB₁ as a means of estimating the degree of dietary exposure to this mycotoxin. In total, 142 samples of maize (n = 54), porridge (47) and faeces (41) were screened for Fusarium spp. using a serial dilution technique followed by DNA sequencing, while FB₁ was further screened and quantified by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC), respectively. At least four species of Fusarium were identified, of which F. verticillioides was the most prevalent in all three sample types analysed. The contamination levels of FB₁ were significantly higher in 87% of maize sampled (range = 101–53,863 µg kg⁻¹) as compared with porridge (74% incidence rate; range = 0.2–20 µg kg⁻¹) and faecal samples (100% incidence rate; range = 0.3–464 µg kg⁻¹). Thus, it can be deduced that the level of human exposure to FB₁ via the consumption of maize was high as several samples contained levels exceeding 1000 µg kg⁻¹, which was strongly supported by the levels found in faecal samples. Further data revealed that a high proportion of FB₁ is destroyed or removed by processing maize into porridge. As maize porridge is consumed as a staple, the low levels found provide a means to limit exposure to FB₁. Levels of FB₁ found in the faeces which were higher indicate that other foods contaminated with the toxin are also consumed.

Keywords:

• mycotoxins
• Fusarium
• commodity
• fumonisin
• rural population
• exposure

Acknowledgements

The authors wish to thank the University of Johannesburg via the New Generation Scholarship (NGS), Medical Research Council (MRC) and National Research Foundation (NRF) of South Africa for funding this study. The contribution of Dr Makun Hussaini and other members of the Food, Environment and Health Research Group (FEHRG) is duly acknowledged. An appreciation is also extended to Chief (Mr Ratshibvumo Ravele) and the population of Mapate Village, Limpopo Province, South Africa, for their support during sampling.