Simultaneous determination of selected biogenic amines in alcoholic beverage samples by isotachophoretic and chromatographic methods

Abstract

A simple and useful method for the determination of biogenic amines in beverage samples based on isotachophoretic separation is described. The proposed procedure permitted simultaneous analysis of histamine, tyramine, cadaverine, putrescine, tryptamine, 2-phenylethylamine, spermine and spermidine. The data presented demonstrate the utility, simplicity, flexibility, sensitivity and environmentally friendly character of the proposed method. The precision of the method expressed as coefficient of variations varied from 0.1% to 5.9% for beverage samples, whereas recoveries varied from 91% to 101%. The results for the determination of biogenic amines were compared with an HPLC procedure based on a pre-column derivatisation reaction of biogenic amines with dansyl chloride. Furthermore, the derivatisation procedure was optimised by verification of concentration and pH of the buffer, the addition of organic solvents, reaction time and temperature.

Graphical Abstract

Keywords:

• biogenic amines
• wine and beer samples
• capillary isotachophoresis
• RP-HPLC

Funding

The author wishes to thank Polish Ministry of Science and Higher Education for financial support [grant number NN 312 465640 (4656/B/P01/2011/40)].