Potentiation of the effect of a commercial animal feed additive mixed with different probiotic yeast strains on the adsorption of aflatoxin B₁

Abstract

This study potentiates the adsorbent effect for aflatoxin B₁ (AFB₁) of a commercial additive (CA) of animal feed, containing inactive lysate of three Saccharomyces cerevisiae strains, active enzymes, adsorbents and a selenium–amino acid complex, when the additive was mixed separately with three S. cerevisiae strains. Levels of AFB₁ of 20 and 50 ng g⁻¹ were used to determine the binding capacity of different concentrations of CA alone and in the presence of yeast strains, as well as toxin desorption, under gastrointestinal conditions. The viability of yeasts in the presence of CA was evaluated. The results show that the CA did not affect the viability of the yeast strains assayed. CA alone showed a low percentage adsorption. At 20 and at 50 ng g⁻¹, CA was highly efficient in adsorbing AFB₁ when combined with RC016 and RC012 strains respectively. Desorption of AFB₁ by CA alone and in combination with the yeasts increased with increasing levels of CA. The results demonstrate the improvement of CA in AFB₁ adsorption once it is mixed with live yeasts.

Graphical Abstract

Keywords:

• animal feed
• AFB₁ binding
• Saccharomyces cerevisiae

Acknowledgements

The authors are grateful to the Consejo Nacional de Ciencia y Técnica (CONICET), the Agencia Nacional de Promoción Científica y Tecnológica (ANPCYT-FONCYT) and the Secretaría de Ciencia y Técnica UNRC. Moreover, they are grateful to VETANCO S.A. for supplying its anti-mycotoxin product to be improved by the addition of yeasts.