Development of high-throughput multi-residue method for non-steroidal anti-inflammatory drugs monitoring in swine muscle by LC-MS/MS

ABSTRACT

A reliable and simple method for the detection and quantification of residues of 14 non-steroidal anti-inflammatory drugs and a metamizole metabolite in swine muscle was developed using liquid chromatography-electrospray ionisation-tandem mass spectrometry (LC-ESI-MS/MS). The samples were extracted with acetonitrile (ACN) in solid–liquid extraction followed by a low-temperature partitioning (LLE-LTP) process at –20 ± 2°C. After evaporation to dryness, the residue was reconstituted with hexane and a mixture of water:acetonitrile (1:1). LC separation was achieved on a reversed-phase (RP₁₈) column with gradient elution using water (phase A) and ACN (phase B) both containing 1 mmol l^–1 ammonium acetate (NH₄COO) with 0.025% acetic acid. Analysis was carried out on a triple-quadrupole tandem mass spectrometer (LC-MS/MS) in multiple reaction monitoring mode using an electrospray interface in negative and positive mode in a single run. Method validation was performed according to the criteria of Commission Decision No. 2002/657/EC. The matrix effect and linearity were evaluated. Decision limit (CCα), detection capability (CCβ), accuracy and repeatability of the method are also reported. The proposed method proved to be simple, easy and adequate for high-throughput analysis and was applied to routine analysis by the Brazilian Ministry of Agriculture, Livestock and Food Supply.

Graphical Abstract

KEYWORDS:

• Non-steroidal anti-inflammatory drugs
• swine muscle
• mass spectrometry
• food safe

Acknowledgements

Fabiano Barreto is Federal Inspector in the Vet Drugs and Pesticides Residues Lab of the National Agricultural Laboratory/RS of the Brazilian Ministry of Agriculture, Livestock and Food Supply. Leonardo Meneghini declares that he has no conflict of interest. Ana Bergold declares that she has no conflict of interest. This article does not contain any studies with human or animal subjects.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

Tamara Santos Castilhos received research grants from the Coordination of Improvement of Higher Education Personnel (CAPES).