Rapid determination of gizzerosine in fish meals using microchip capillary electrophoresis with laser-induced fluorescence detection

ABSTRACT

Sensitive detection of gizzerosine, a causative agent for deadly gizzard erosion in chicken feeds, is very important to the poultry industry. In this work, a new method was developed based on microchip capillary electrophoresis (MCE) with laser-induced fluorescence (LIF) detection for rapid analysis of gizzerosine, a biogenic amine in fish meals. The MCE separation was performed on a glass microchip using sodium dodecyl sulfate (SDS) as dynamic coating modifier. Separation conditions, including running buffer pH and concentration, SDS concentration, and the separation voltage were investigated to achieve fast and sensitive quantification of gizzerosine. The assay proposed was very quick and could be completed within 65 s. A linear calibration curve was obtained in the range from 0.04 to 1.8 μg ml^–1 gizzerosine. The detection limit was 0.025 μg ml^–1 (0.025 mg kg^–1), which was far more sensitive than those previously reported. Gizzerosine was well separated from other endogenous components in fish meal samples. Recovery of gizzerosine from this sample matrix (n = 3) was determined to be 97.2–102.8%. The results from analysing fish meal samples indicated that the present MCE-LIF method might hold the potential for rapid detection of gizzerosine in poultry feeds.

KEYWORDS:

• Microchip capillary electrophoresis
• laser-induced fluorescence
• gizzerosine
• surface coating
• poultry feeds

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the State Key Laboratory of Phytochemistry and Plant Resources in West China [grant number P2015-KF12]; the West Light Foundation of The Chinese Academy of Sciences; and the National Natural Science Foundation of China [grant number 81173536].