Fumonisin B₁ induced compositional modifications of the renal and hepatic membrane lipids in rats – Dose and exposure time dependence

ABSTRACT

Male Wistar rats were intraperitoneally dosed with fumonisin B1 (FB₁; 0, 20, 50 and 100 mg kg⁻¹ dietary dose equivalent) for 5 & 10 days to assess dose- and time-dependent effects on renal and hepatic phosphatidylcholine (PC), phosphatidylinositol (PI) and phosphatidylethanolamine (PE) fatty acid (FA) profiles. Renal PC showed increasing FA saturation (SAT) after 5 days; after 10 days polyunsaturation (PUFA) decreased markedly (Σ n3 (total n3), Σ n6, PUFA, unsaturation index (UI) and average FA chain length (ACL)), mostly with linear dose response. In the PI FAs similar changes were observed, decreasing monounsaturated FA, PUFA, UI and ACL (5 & 10 days), while the PE fraction was responsive in Σ n6 (↓) and SAT (↑), but only after 5 days (without dose response for both PI & PE). Liver PC exhibited increasing saturation (C16:0), decreasing polyunsaturation (C20:3 n6 [dihomo-γ-linolenic acid, DGLA]; C20:3 n3); the PI FA profile showed similar alterations after 5 days. PC & PI FA failed to respond in a dose-dependent manner to FB₁. In PE FA profile DGLA decreased, with a decrease of the total n6 FA proportion and dose-dependent increase of n3 FAs. Results revealed expressed renal sensitivity, supporting our earlier published results in terms of oxidative stress and histopathological modifications.

KEYWORDS:

• Fumonisin B₁
• rat
• kidney
• liver
• membrane lipid
• fatty acids

Conflict of interest

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Additional information

Funding

This research was funded by the Ministry of Innovation and Technology [GINOP-2.3.2-15-2016-00046] and the Ministry of Human Resources [EFOP-3.6.3-VEKOP-16-2017-00005]. The research was further funded by Hungarian Academy of Sciences [MTA-KE-SZIE 13003].