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Articles

Potential ways for gluten contamination of gluten-free grain and gluten-free foods: the buckwheat case

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Pages 1591-1600 | Received 05 May 2020, Accepted 21 Jun 2020, Published online: 17 Aug 2020
 

ABSTRACT

Buckwheat has been reported to be responsible for gluten contamination in manufactured gluten-free foods (mGFFs) although it is inherently gluten-free (GF). It could happen through buckwheat grains contacting gluten-containing (GC) grains and surfaces contacted by GC grains during pre-manufacturing practices. To simulate grain contact, whole and broken GC grains (wheat, rye, barley, and oat) were mixed into buckwheat grains at the ratio of 2.5–10.0%. Grains were agitated in vessels with inner surfaces covered with buckwheat grain. Gluten was not detected in buckwheat grains contacting whole GC grains at all mixing ratios. It was not detected in the case of broken GC grains at the mixing ratio of 2.5% and oat grains at all mixing ratios. Gluten concentration increased with the increasing mixing ratio and the natural gluten concentration of broken GC grains. To simulate surface contact, GC grains were first agitated in galvanised steel vessels and then buckwheat grains were agitated together under the same conditions. Gluten was detected on galvanised steel surfaces contacted by whole and broken GC grains. It was not detected in buckwheat grains contacting the surfaces contaminated by whole GC grains. Gluten was detected in buckwheat grain in the case of the broken GC grains except for oats. Gluten concentrations increased with increasing natural gluten concentration of GC grains. Contamination of mGFFs could be linked to potential contact with buckwheat grain. This contamination issue could be resolved through regulations mandating the proof of being GF for ingredients used in the production of mGFFs.

Graphical abstract

Acknowledgments

Authors dedicate this work to gluten sufferers and their families by appreciating how a GF-life is challenging, and gratefully acknowledge the support of University of Mersin under the Project 2018-TP2-2818.

Declaration of interest statement

We, the authors of the manuscript, are reporting that we have no potential conflict of interest with any party including but not limited to any person, corporation, organization, and institution.

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