Establishment of an immunological detection method of fleroxacin by fluorescence-linked immunosorbent assay

ABSTRACT

The direct and indirect competitive fluorescence-linked immunosorbent assay (FLISA and icFLISA) incorporating quantum dots (QDs) for the detection of fleroxacin (FLE) was established for the first time in this study. The monoclonal antibody specific for FLE was successfully conjugated with QDs after purification by the caprylic acid-ammonium sulphate method. The limits of detection of FLISA and icFLISA were 0.012 ng/mL and 0.006 ng/mL, respectively; IC₅₀ were 0.32 ng/mL and 0.19 ng/mL; and the detection ranges were 0.012–24.490 ng/mL and 0.006–16.210 ng/mL. The recovery was 93.8%-112.4% and the coefficient of variation was below 11.75%. The fabricated FLISA and icFLISA are cost-effective, high sensitive and can be an alternative method in the detection of FLE residues.

Graphical Abstract

KEYWORDS:

• Fleroxacin
• quantum dots
• monoclonal antibody
• FLISA
• icFLISA

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This study was supported by the National Key Research and Development Program [2019YFC1604501]; The ‘1125 talent gathering plan’ project of Zhengzhou.