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Original Articles

Development of an ultrasensitive spectrophotometric method for carmine determination based on fluorescent carbon dots

, , , , , , & show all
Pages 731-740 | Received 12 Jan 2021, Accepted 02 Feb 2021, Published online: 08 Mar 2021
 

ABSTRACT

A high-efficiency spectrophotometric method based on nitrogen-doped fluorescent carbon dots (N-FCDs) was developed for the ultrasensitive determination of carmine (CRM) in foodstuffs. The N-FCDs were fabricated via a one-pot hydrothermal method with m-phenylenediamine as the starting material. The detection principle was based on the fluorescence quenching effect of N-FCDs by CRM, where their interaction was due to the inner filter effect (IFE) and static quenching. A good linear relationship was established for CRM detection in a concentration range of 0.1–10.0 μM with a detection limit as low as 11.2 nM. The proposed method achieved satisfactory results for CRM determination in commercial food products with recoveries better than 98.6% and relative standard deviations (RSDs) less than 4.07%. The method established in this study was simple, ultrasensitive and reliable for rapid detecting CRM in a food matrix, which could be potentially used as a useful sensing agent for the analysis of additive food colourants.

Graphical Abstract

Supplemental data

Supplemental data for this article can be accessed on the publisher’s website.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the National Natural Science Foundation of China [21922202]; Jiangsu Key Research & Development Program, China [BE2019436-5]; LvYang Golden Phoenix Plan, Yangzhou City [2019]; Natural Science Fund for Colleges and Universities in Jiangsu Province, China [19KJB150042,20KJA550002]; Yangzhou Key R&D Project (Social Development), China (2020) [YZ2020063]; China Postdoctoral Science Foundation [2020M671625]; Opening Foundation of Jiangsu Dairy Bioengineering Technology Research Centre [KYRY2019002]; Natural Science Foundation of Jiangsu Province, China [BK20200949]; Yangzhou University Science and Technology Innovation Cultivation Fund [2019CXJ188]; National Key Research and Development Program of China [2016YFC1300201].

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