Abstract
Hainanmycin is a polyether antibiotic. Toxicological studies have shown the adverse effects of hainanmycin on animals and humans. At present, no study is available on the detection of hainanmycin in edible tissues of animals. Hence, a fast and accurate detection method for hainanmycin is essential. This study aimed to develop a new analytical method based on ultra-high-performance liquid chromatography-tandem mass spectrometry to detect hainanmycin in 10 matrices, including milk, eggs, fat, kidney, muscles and livers of chicken, beef and sheep. The limit of detection and the limit of quantitation of the 10 matrices were 0.1–0.4 μg/kg and 0.25–1 μg/kg, respectively, and were far below the maximum residue limits of other polyether anticoccidial drugs (1–150 μg/kg). The recoveries of hainanmycin ranged from 79% to 105%, and the relative standard deviation ranged from 2.8% to 12.0%. The research results prove that the proposed method is operational and simple in detecting hainanmycin, and has high precision and accuracy in a variety of matrices.
Author contributions
Ke-xin Chi: Conceptualization, methodology, investigation, writing – original draft, supervision, funding acquisition, and project administration. Yun-fei Xie: Investigation, methodology, and data curation. Hang Yu: Validation. Ya-hui Guo: Validation, and resources. Yu-liang Cheng: Supervision. Wei-rong Yao: Conceptualization, methodology, funding acquisition, and writing – review and editing.
Disclosure statement
The authors have no conflicts of interest concerning the submission of this manuscript. They declare no known competing financial interests or personal relationships that could have appeared to influence the work reported in this study.