DNA changes in Pseudomonas putida induced by aluminum oxide nanoparticles using RAPD analysis

Abstract

This research has been conducted to determine the genotoxicity of nano-Al₂O₃ towards Pseudomonas putida bacteria using RAPD-PCR method. The results were compared with the impact of the macro form of the compound on DNA. On the basis of RAPD profiles using the primer OPA2, the research demonstrated a mutagenic action of the tested nanocompound. The obtained profiles of RAPD bands are different from the negative control by over 30–9.1%. For the products of PCR carried out using the primer OPA9, genetic similarity indexes relative to the negative control for nano-Al₂O₃ samples were 80% in all tested concentrations. Regarding the remaining primers, RAPD bands profiles for the samples with the nanocompound and the negative control varied only to a small extent. This study showed a decrease in the genetic stability of DNA (GTS, %) after treatment of the bacteria nano-Al₂O₃ as compared with the negative control. GTS value for the nano-Al₂O₃ was 65% (1,000 mg/L). In the remaining concentrations of nano-Al₂O_3, GTS remained in the range of 75–80%. The results showed that the nano-Al₂O₃ can induce modifications of the genetic material to a greater extent than the same compounds in the macro form.

Keywords:

• Aluminum oxide nanoparticles
• RAPD-PCR
• Genotoxicity

Notes

Presented at the 12th Scientific Conference on Microcontaminants in Human Environment 25–27 September 2014, Czestochowa, Poland