Mechanical emulsification of lipoaspirate by different Luer-Lok connector changes the viability of adipose derived stem cells in Nanofat

Abstract

Nanofat grafting is a fat transfer procedure that uses a thin needle to smooth out wrinkles, thereby achieving the goal of skin rejuvenation. The Luer-Lok connector is one of the most common methods for obtaining Nanofat. In the present study, we compared three different Luer-Lok connectors (2.0 mm, 1.5 mm and 1.1 mm in diameter) in terms of their impact on the viability of adipose-derived stem cells (ADSCs) to determine the optimal size of the connector for efficient Nanofat grafting. We observed that a smaller diameter of the Luer-Lok connector created a higher mechanical shear force, which broke more fat cells during the emulsifying procedure, thereby reducing the viability of ADSCs from the stromal vascular fraction (SVF). Nanofat obtained from the 2-mm Luer-Lok connector had a better effect on skin rejuvenation than the 1.5-mm and 1.1-mm connectors. Therefore, this study presents an advance in the simple procedure of preparing Nanofat based on a previous technique and provides evidence that a procedure associated with less trauma may be a better choice.

Keywords:

• Nanofat grafting
• adipose derived stem cells
• Luer-Lok connector
• skin rejuvenation

Acknowledgements

The authors thank Dr. Jingjing Hou and Dr. Naiqiang Guo for critical reading of the manuscript.

Disclosure statement

All of the authors declared no potential conflicts of interest with respect to the research, authorship and publication of this article.

Additional information

Funding

This work was supported by The Fujian Health Administration Grants [No. 2017-2-102]. This work was also supported by The National Natural Science Foundation of China [No. 31701201] and Xiamen Municipal Bureau of Science and Technology Grant [No. 3502Z20174079].