Environmental impacts of genetically modified (GM) crop use 1996–2013: Impacts on pesticide use and carbon emissions

ABSTRACT

This paper updates previous assessments of how crop biotechnology has changed the environmental impact of global agriculture. It focuses on the environmental impacts associated with changes in pesticide use and greenhouse gas emissions arising from the use of GM crops since their first widespread commercial use in the mid 1990s. The adoption of GM insect resistant and herbicide tolerant technology has reduced pesticide spraying by 553 million kg (−8.6%) and, as a result, decreased the environmental impact associated with herbicide and insecticide use on these crops (as measured by the indicator the Environmental Impact Quotient (EIQ)) by19.1%. The technology has also facilitated important cuts in fuel use and tillage changes, resulting in a significant reduction in the release of greenhouse gas emissions from the GM cropping area. In 2013, this was equivalent to removing 12.4 million cars from the roads.

Keywords:

• GMO
• pesticide
• active ingredient
• environmental impact quotient
• carbon sequestration
• biotech crops
• no tillage

Abbreviations

GM	=	genetic modification
EIQ	=	environmental impact quotient
HT	=	herbicide tolerant
IR	=	insect resistant
ALS inhibitor	=	herbicide that inhibits the acetolactate synthase enzyme production (in weeds)
RT	=	reduced tillage
NT	=	no tillage

Introduction

GM crop technology has been widely used since the mid 1990s in a number of countries and has mainly been used in 4 main crops; canola, maize, cotton and soybean. In 2013, crops containing this type of technology accounted for 46% of the global plantings of these crops. In addition, small areas of GM sugar beet (adopted in the USA and Canada since 2008), papaya (in the USA since 1999 and China since 2008), alfalfa (in the US initially in 2005–2007 but latterly since 2011) and squash (in the USA since 2004) have also been planted.

The main traits so far commercialised convey:

• Tolerance to specific herbicides (notably to glyphosate and to glufosinate) in maize, cotton, canola (spring oilseed rape), soybean, sugar beet and alfalfa. This GM herbicide tolerant (GM HT) technology allows for the ‘over the top’ spraying of GM HT crops with these specific broad-spectrum herbicides, that target both grass and broad-leaved weeds but do not harm the crop itself;

• Resistance to specific insect pests of maize and cotton. This GM insect resistance (GM IR), or ‘Bt’ technology offers farmers resistance in the plants to major pests such as stem and stalk borers, earworms, cutworms and rootworm (e.g., Ostrinia nubilalis, Ostrinia furnacalis, Spodoptera frugiperda, Diatraea spp, Helicoverpa zea and Diabrotica spp) in maize and bollworm/budworm (Heliothis sp and Helicoverpa) in cotton.

In addition, the GM papaya and squash referred to above are resistant to important viruses (e.g., ringspot in papaya) and GM IR soybeans were adopted for the first time in 2013, in South America.

This paper presents an assessment of some of the key environmental impacts associated with the global adoption of these GM traits. The environmental impact analysis focuses on:

• Changes in the amount of insecticides and herbicides applied to the GM crops relative to conventionally grown alternatives and;

• The contribution of GM crops toward reducing global greenhouse gas (GHG) emissions.

It is widely accepted that increases in atmospheric levels of greenhouse gases such as carbon dioxide, methane and nitrous oxide are detrimental to the global environment (see, for example, Intergovernmental Panel on Climate Change, 2006). Therefore, if the adoption of crop biotechnology contributes to a reduction in the level of greenhouse gas emissions from agriculture, this represents a positive development for the world.

The study integrates data for 2013 into the context of earlier developments and updates the findings of earlier analysis presented by the authors in AgbioForum 8 (2 & 3) 187–196 (Brookes and Barfoot, 2006), 9 (3) 1–13 (Brookes and Barfoot, 2007), 11 (1), 21–38 (Brookes and Barfoot, 2008) and 13 (1), 76–94 (Brookes and Barfoot, 2010) and GM Crops (2011), vol 12, issue 1, 34–49, (2012) 3: 2 April-June, p 1–9, (2013) 4:2 April–June, p 1–11 and (2014) 5:2, 149–160 April-June (Brookes and Barfoot, 2011, 2012, 2013, 2014).

The methodology and analytical procedures in this present discussion are unchanged to allow a direct comparison of the new with earlier data (readers should however, note that some data presented in this paper are not directly comparable with data presented in previous analysis because the current paper takes into account the availability of new data and analysis, including revisions to data for earlier years), and in order to save readers the chore of consulting these earlier papers for details of the methodology and arguments, these elements are included in full in this updated paper.

The aim of this has been to provide an up to date and as accurate as possible assessment of some of the key environmental impacts associated with the global adoption of GM crops. It is also hoped the analysis continues to make a contribution to greater understanding of the impact of this technology and facilitates more informed decision making, especially in countries where crop biotechnology is currently not permitted.

Results and Discussion

Results: Environmental impacts of insecticide and herbicide use changes

HT crops

A key impact of GM HT (largely tolerant to glyphosate) technology use has been a change in the profile of herbicides typically used. In general, a fairly broad range of, mostly selective (grass weed and broad-leaved weed) herbicides has been replaced by one or 2 broad-spectrum herbicides (mostly glyphosate) used in conjunction with one or 2 other (complementary) herbicides (e.g., 2 4,D). This has resulted in:

• Aggregate reductions in both the volume of herbicides used (in terms of weight of active ingredient applied) and the associated field EIQ values when compared to usage on conventional (non GM) crops in many countries, indicating net improvements to the environment (for an explanation of the EIQ indicator, see the methodology section);

• In some countries, the average amount of herbicide active ingredient applied to GM HT crops represents a net increase relative to usage on the conventional crop alternative. However, even though the amount of active ingredient use has increased, in terms of the associated environmental impact, as measured by the EIQ indicator, the environmental profile of the GM HT crop has commonly been better than its conventional equivalent;

• Where GM HT crops (tolerant to glyphosate) have been widely grown, some incidence of weed resistance to glyphosate has occurred (see additional discussion below) and has become a major problem in some regions (see www.wee\dscience.org). This can be attributed to how glyphosate was used; because of its broad-spectrum post-emergence activity, it was often used as the sole method of weed control. This approach to weed control put tremendous selection pressure on weeds and as a result contributed to the evolution of weed populations predominated by resistant individual weeds. In addition, the facilitating role of GM HT technology in the adoption of RT/NT production techniques in North and South America has also probably contributed to the emergence of weeds resistant to herbicides like glyphosate and to weed shifts toward those weed species that are not inherently well controlled by glyphosate. As a result, growers of GM HT crops are increasingly being advised to include other herbicides (with different and complementary modes of action) in combination with glyphosate and in some cases to revert to ploughing in their integrated weed management systems. At the macro level, these changes have already begun to influence the mix, total amount, cost and overall profile of herbicides applied to GM HT crops. Compared to several years ago, the amount of herbicide active ingredient applied and number of herbicides used with GM HT crops in many regions has increased, and the associated environmental profile, as measured by the EIQ indicator, deteriorated. This increase in herbicide use relative to several years ago is often cited by anti GM technology proponents (Benbrook, 2012) as an environmental failing of the technology. However, what such authors fail to acknowledge is that the amount of herbicide used on conventional crops has also increased relative to several years ago and that compared to the conventional alternative, the environmental profile of GM HT crop use has continued to offer important advantages and in most cases, provides an improved environmental profile compared to the conventional alternative (as measured by the EIQ indicator; Brookes et al., 2012). It should also be noted that many of the herbicides used in conventional production systems had significant resistance issues themselves in the mid 1990s. This was, for example, one of the reasons why glyphosate tolerant soybean technology was rapidly adopted, as glyphosate provided good control of these weeds.

• These points are further illustrated in the analysis below

GM HT soybean

The environmental impact of herbicide use change associated with GM HT soybean adoption is summarised in Table 1. Overall, there has been a small net decrease in the amount of herbicide active ingredient used (−0.1%), which equates to about 2.3 million kg less active ingredient applied to these crops than would otherwise have occurred if a conventional crop had been planted. The environmental impact, as measured by the EIQ indicator, nevertheless, improved by a more significant 14.5% due to the increased usage of more environmentally benign herbicides.

TABLE 1. GM HT Soybean: Summary of active ingredient usage and associated EIQ changes 1996–2013

Country	Change in active ingredient use (million kg)	% change in amount of active ingredient used	% change in EIQ indicator
Romania (to 2006 only)	−0.02	−2.1	−10.5
Argentina	−1.5	−0.2	−9.6
Brazil	+30.0	+3.3	−4.9
US	−32.3	−3.8	−24.9
Canada	−2.5	−8.1	−22.9
Paraguay	+2.8	+5.0	−5.4
Uruguay	+0.45	+2.3	−8.2
South Africa	−0.1	1.2	−17.2
Mexico	−0.02	−1.0	−5.0
Bolivia	+0.9	+6.0	−3.2
Aggregate impact: all countries	−2.29	−0.1	−14.5

Note: Negative sign = reduction in usage or EIQ, positive sign = increase in usage or EIQ value.

At the country level, most user countries recorded both a net reduction in the use of herbicide active ingredient and an improvement in the associated environmental impact, as measured by the EIQ indicator. The exceptions to this have been Brazil, Bolivia, Paraguay and Uruguay, where there have been net increases in the amount of herbicide active ingredient applied, though the overall environmental impact, as measured by the EIQ indicator has been positive. The largest environmental gains have tended to be in developed countries where the usage of herbicides has traditionally been highest and where there has been a significant movement away from the use of several selective herbicides to one broad spectrum herbicide plus one or 2 additional, complementary herbicides targeted at weeds that are difficult to control with glyphosate.

In 2013, the amount of herbicide active ingredient applied to the global GM HT soybean crop increased by 6.8 million kg (+3.1%) relative to the amount reasonably expected if this crop area had been planted to conventional cultivars. This highlights the point above relating to recent increases in herbicide use with GM HT crops to take account of weed resistance issues. However, despite these increases in the volume of active ingredient used, in EIQ terms, the environmental impact of the 2013 GM HT soybean crop continued to represent an improvement relative to the conventional alternative (a 9.3% improvement).

GM HT maize

The adoption of GM HT maize has resulted in a significant reduction in both the volume of herbicide active ingredient usage and the associated environmental impact, as measured by the EIQ indicator (Table 2).

TABLE 2. GM HT maize: Summary of active ingredient usage and associated EIQ changes 1996–2013

Country	Change in active ingredient use (million kg)	% change in amount of active ingredient used	% change in EIQ indicator
US	−190.4	−10.3	−14.5
Canada	−8.1	−15.5	−18.2
Argentina	−3.7	−3.5	−8.2
South Africa	−1.6	−1.8	−5.7
Brazil	−6.6	−3.6	−7.9
Uruguay	−0.1	−5.7	−11.9
Aggregate impact: all countries	−210.5	−9.3	−13.5

Note:

1. Negative sign = reduction in usage or EIQ, positive sign = increase in usage or EIQ value.

2. Other countries using GM HT maize – Colombia and the Philippines, not included due to lack of data. Also, hand weeding is likely to be an important form of weed control suggesting any reduction in herbicide use with GM HT maize has been limited.

In 2013, the reduction in herbicide usage was just over 21.8 million kg of active ingredient (−9.8%), with a larger reduction in the EIQ indicator of 14.6%. As with GM HT soybeans, the greatest environmental gains have been in developed countries (e.g., the US and Canada), where the usage of herbicides has traditionally been highest.

GM HT cotton

The use of GM HT cotton delivered a net reduction in herbicide active ingredient use of about 21.3 million kg over the 1996–2013 period. This represents a 7.2% reduction in usage, although in terms of the EIQ indicator, the change has been a higher 9.5% reduction (ie, there has been a net environmental improvement). In 2013, the use of GM HT cotton technology resulted in a 2.9 million kg reduction in herbicide active ingredient use (−5.6%) and an 18.9% reduction in the field EIQ indicator value (Table 3).

TABLE 3. GM HT cotton summary of active ingredient usage and associated EIQ changes 1996–2013

Country	Change in active ingredient use (million kg)	% change in amount of active ingredient used	% change in EIQ indicator
US	−14.8	−5.7	−7.7
South Africa	+0.01	+1.1	−7.4
Australia	−2.0	−9.2	−12.4
Argentina	−4.5	−28.0	−32.5
Aggregate impact: all countries	−21.3	−7.2	−9.5

Note:

1. Negative sign = reduction in usage or EIQ, positive sign = increase in usage or EIQ value.

2. Other countries using GM HT cotton – Brazil, Colombia and Mexico, not included due to lack of data.

Other HT crops

GM HT canola (tolerant to glyphosate or glufosinate) has been grown in Canada, the US, and more recently Australia, while GM HT sugar beet is grown in the US and Canada. The environmental impacts associated with changes in herbicide usage on these crops are summarised in Table 4. GM HT canola use has resulted in significant reductions in both the amount of herbicide active ingredient used and the associated field EIQ indicator.

TABLE 4. Other GM HT crops summary of active ingredient usage and associated EIQ changes 1996–2013

Country	Change in active ingredient use (million kg)	% change in amount of active ingredient used	% change in EIQ indicator
GM HT canola
US	−2.2	−29.9	−42.7
Canada	−15.8	−17.9	−29.7
Australia	−0.4	−2.3	−1.8
Aggregate impact: all countries	−18.4	−16.5	27.9
GM HT sugar beet
US and Canada	+1.7	+31.2	−0.8

Note:

1. Negative sign = reduction in usage or EIQ, positive sign = increase in usage or EIQ value.

2. In Australia, one of the most popular type of production has been canola tolerant to the triazine group of herbicides (tolerance derived from non GM techniques). It is relative to this form of canola that the main farm income benefits of GM HT (to glyphosate) canola has occurred.

3. InVigor’ hybrid vigour canola (tolerant to the herbicide glufosinate) is higher yielding than conventional or other GM HT canola and derives this additional vigour from GM techniques.

4. GM HT alfalfa is also grown in the US. The changes in herbicide use and associated environmental impacts from use of this technology is not included due to a lack of available data on herbicide use in alfalfa.

In respect of GM HT sugar beet, the change in herbicide usage away from several selective herbicides to a fewer applications of, often a single herbicide (glyphosate) has resulted in an increase in the total volume of herbicides applied to the sugar beet crop but a small net improvement in the associated environmental impact (−0.8%).

In 2013, the use of GM HT canola resulted in a 2.1 million kg reduction in the amount of herbicide active ingredient use (−17.1%), with an improvement in the environmental impact, as measured by the EIQ indicator of 31.5%. For GM HT sugar beet, an additional 0.37 million kg of herbicide active ingredient was applied to the sugar beet crops in the US and Canada (+41%). This also resulted in a small net deterioration in the associated environmental impact (−6.5%: as measured by the EIQ indicator).

Weed resistance

As indicated above, weed resistance to glyphosate has become a major issue affecting some farmers using GM HT (tolerant to glyphosate) crops. Worldwide there are currently (accessed February 2015) 32 weeds species resistant to glyphosate of which several are not associated with glyphosate tolerant crops (www.weedscience.org). In the US, there are currently 14 weeds recognized as exhibiting resistance to glyphosate, of which 2 are not associated with glyphosate tolerant crops. In addition, some of the first glyphosate resistant weeds developed in Australia in the mid 1990s before the adoption of GM HT crops and currently there are 10 weeds exhibiting resistance to glyphosate in Australia, even though the area using GM HT (tolerant to glyphosate) crops in the country is relatively small (about 0.64 million ha in 2013). In Argentina, Brazil and Canada, where GM HT crops are widely grown, the number of weed species exhibiting resistance to glyphosate are respectively 7, 6 and 5. A few of the glyphosate-resistant species, such as marestail (Conyza canadensis), waterhemp (Amaranthus tuberculatus) and palmer pigweed (Amaranthus palmeri) in the US, are now reasonably widespread, with the affected area being possibly within a range of 30%–40% of the total area annually devoted to maize, cotton and soybeans.

This resistance development should, however, be placed in context. All weeds have the ability to develop resistance to all herbicides and there are hundreds of resistant weed species confirmed in the International Survey of Herbicide Resistant Weeds (www.weedscience.org), and reports of herbicide resistant weeds pre-date the use of GM HT crops by decades. There are, for example, 146 weed species that are resistant to ALS herbicides and 72 weed species resistant to photosystem II inhibitor herbicides.

Where farmers are faced with the existence of weeds resistant to glyphosate in GM HT crops, they are increasingly being advised to be more proactive and include other herbicides (with different and complementary modes of action) in combination with glyphosate and in some cases to revert to ploughing in their integrated weed management systems. This change in weed management emphasis also reflects the broader agenda of developing strategies across all forms of cropping systems to minimize and slow down the potential for weeds developing resistance to existing technology solutions for their control. At the macro level, these changes have already begun to influence the mix, total amount, cost and overall profile of herbicides applied to GM HT crops.

For example, in the 2013 US GM HT soybean crop, 64% of the GM HT soybean crop received an additional herbicide treatment of one of the following (4 most used, after glyphosate) active ingredients 2,4-D (used pre crop planting), chlorimuron, flumioxazin and sulfentrazone (each used primarily after crop planting). This compares with 14% of the GM HT soybean crop receiving a treatment of one of these 4 herbicide active ingredients in 2006. As a result, the average amount of herbicide active ingredient applied to the GM HT soybean crop in the US (per hectare) increased by about 64% over this period. The increase in non-glyphosate herbicide use is primarily in response to public and private sector weed scientist recommendations to diversify weed management programmes and not to rely on a single herbicide mode of action for total weed management. It is interesting to note that in 2013, glyphosate accounted for a slightly less share of total active ingredient use on the GM HT crop (77%) as in 1998 when it accounted for 82% of total active ingredient use, highlighting that farmers continue to realize value in using glyphosate because of its broad spectrum activity in addition to using other herbicides. On the small conventional crop, the average amount of herbicide active ingredient applied increased by 64% over the same period reflecting a shift in herbicides used rather than increased dose rates for some herbicides. The increase in the use of herbicides on the conventional soybean crop in the US can also be partly attributed to the on-going development of weed resistance to non-glyphosate herbicides commonly used and highlights that the development of weed resistance to herbicides is a problem faced by all farmers, regardless of production method. It is also interesting to note that since the mid 2000s, the average amount of herbicide active ingredient used on GM HT cotton in the US has increased through a combination of additional usage of glyphosate (about a 30% increase in usage per hectare) in conjunction with increasing use of other herbicides. All of the GM HT crop area planted to seed tolerant to glyphosate received treatments of glyphosate and at least one of the next 5 most used herbicides (trifluralin, acetochlor, S metolachlor, fomesafen and pendimethalin). This compares with 2006, when only 3-quarters of the glyphosate tolerant crop received at least one treatment from the next 5 most used herbicides (2 4-D, trifluralin, pyrithiobic, pendimethalin and diuron). In other words, a quarter of the glyphosate tolerant crop used only glyphosate for weed control in 2006 compared to none of the crop relying solely on glyphosate in 2013. This suggests that US cotton farmers are increasingly adopting current/recent recommended practices for managing weed resistance (to glyphosate).

Relative to the conventional alternative, the environmental profile of GM HT crop use has, nevertheless, continued to offer important advantages and in most cases, provides an improved environmental profile compared to the conventional alternative (as measured by the EIQ indicator).

GM IR crops

The main way in which these technologies have impacted on the environment has been through reduced insecticide use (Tables 5 and 6). While the adoption of GM HT crops resulted in a shift in the profile of herbicides used, the GM IR technology has effectively replaced insecticides used to control important crop pests. This is particularly evident in respect of cotton, which traditionally has been a crop on which intensive treatment regimes of insecticides were common place to control bollworm/budworm pests. In maize, the insecticide use savings have tended to be more limited because the pests that the various technology targets tend to be less widespread in maize than budworm/bollworm pests are in cotton. In addition, insecticides were widely considered to have limited effectiveness against some pests in maize crops (e.g., stalk borers) because the pests can be found in places where sprays are not effective (e.g., inside stalks). As a result of these factors, the proportion of the maize crop in most GM IR user countries that typically received insecticide treatments before the availability of GM IR technology was much lower than the share of the cotton crops receiving insecticide treatments (e.g., in the US, no more than 10% of the maize crop typically received insecticide treatments targeted at stalk boring pests and about 30%–40% of the crop annually received treatments for rootworm).

TABLE 5. GM IR maize: Summary of active ingredient usage and associated EIQ changes 1996–2013

Country	Change in active ingredient use (million kg)	% change in amount of active ingredient used	% change in EIQ indicator
US	−56.5	−48.5	−47.5
Canada	−0.63	−87.7	−62.0
Spain	−0.5	−35.6	−20.2
South Africa	−1.4	−63.6	−63.6
Brazil	−12.5	−84.4	−84.4
Colombia	−0.12	−58.0	−58.0
Aggregate impact: all countries	−71.65	−52.8	−53.1

Note:

1. Negative sign = reduction in usage or EIQ, positive sign = increase in usage or EIQ value.

2. Other countries using GM IR maize – Argentina, Uruguay, Honduras and the Philippines, not included due to lack of data and/or little or no history of using insecticides to control various pests.

3. % change in active ingredient usage and field EIQ values relates to insecticides typically used to target lepidopteran pests (and rootworm in the US and Canada) only. Some of these active ingredients are, however, sometimes used to control to other pests that the GM IR technology does not target.

TABLE 6. GM IR cotton: Summary of active ingredient usage and associated EIQ changes 1996–2013

Country	Change in active ingredient use (million kg)	% change in amount of active ingredient used	% change in EIQ indicator
US	−14.1	−19.3	−17.0
China	−118.0	−30.2	−30.7
Australia	−17.8	−33.1	−33.9
India	−74.4	−24.5	−31.4
Mexico	−1.4	−11.0	−10.9
Argentina	−3.7	−63.2	−48.1
Brazil	−0.7	−10.2	−13.8
Aggregate impact: all countries	−230.1	−27.2	−29.6

Note:

1. Negative sign = reduction in usage or EIQ, positive sign = increase in usage or EIQ value.

2. Other countries using GM IR cotton – Colombia, Burkina Faso, Pakistan and Burma not included due to lack of data.

3. % change in active ingredient usage and field EIQ values relates to all insecticides (as bollworm/budworm pests are the main category of cotton pests worldwide). Some of these active ingredients are, however, sometimes used to control to other pests that that the GM IR technology does not target.

The global insecticide savings from using GM IR maize and cotton in 2013 were, 8.2 million kg (−83.8% of insecticides typically targeted at maize stalk boring and rootworm pests) and 21.3 million kg (−48.3% of all insecticides used on cotton) respectively of active ingredient use. In EIQ indictor terms, the respective savings in 2013 were 88.9% for insecticides targeted at maize stalk boring and rootworm pests and 48.3% for total cotton insecticides. Cumulatively since 1996, the gains have been a 71.7 million kg reduction in maize insecticide active ingredient use and a 230.1 million kg reduction in cotton insecticide active ingredient use.

In 2013 IR soybeans were also first grown commercially in South America (mostly Brazil). In this first year of use on about 2.3 million hectares, the insecticide use (active ingredient) saving has been just over 0.4 million kg (1% of total soybean insecticide use), with the associated EIQ saving being about 2.8%.

Aggregated (global level) impacts

At the global level, GM technology has contributed to a significant reduction in the environmental impact associated with insecticide and herbicide use on the areas devoted to GM crops. Since 1996, the use of pesticides on the GM crop area was reduced by 553 million kg of active ingredient (an 8.6% reduction), and the environmental impact associated with herbicide and insecticide use on these crops, as measured by the EIQ indicator, fell by 19.1%. In 2013, the environmental benefit was equal to a reduction of 49.5 million kg of pesticide active ingredient use (−8.6%), with the environmental impact associated with insecticide and herbicide use on these crops, as measured by the EIQ indicator, falling by 19.5%.

At the country level, US farms have seen the largest environmental benefits, with a 309 million kg reduction in pesticide active ingredient use (56% of the total). This is not surprising given that US farmers were first to make widespread use of GM crop technology, and for several years, the GM adoption levels in all 4 US crops have been in excess of 80%, and insecticide/herbicide use has, in the past been, the primary method of weed and pest control. Important environmental benefits have also occurred in China and India from the adoption of GM IR cotton, with a reduction in insecticide active ingredient use of over 192 million kg (1996–2013).

Results: Greenhouse gas emission savings

Reduced fuel use

The fuel savings associated with making fewer spray runs in GM IR crops of maize and cotton (relative to conventional crops) and the switch to reduced tillage or no tillage (RT/NT) farming systems facilitated by GM HT crops, have resulted in permanent savings in carbon dioxide emissions. In 2013, this amounted to a saving of about 2,096 million kg of carbon dioxide, arising from reduced fuel use of 785 million liters (Table 7). These savings are equivalent to taking 0.93 million cars off the road for one year.

TABLE 7. Carbon storage/sequestration from reduced fuel use with GM crops 2013

Crop/trait/country	Fuel saving (million liters)	Permanent carbon dioxide savings arising from reduced fuel use (million kg of carbon dioxide)	Permanent fuel savings: as average family car equivalents removed from the road for a year (‘000s)
US: GM HT soybean	79	210	93
Argentina: GM HT soybean	281	751	334
Brazil GM HR soybean	171	456	203
Bolivia, Paraguay, Uruguay: GM HT soybean	63	169	75
US: GM HT maize	79	211	94
Canada: GM HT canola	69	185	82
Global GM IR cotton	13	34	15
Brazil IR maize	30	80	36
Total	785	2,096	931

Note:

1. Assumption: an average family car produces 150 g of carbon dioxide per km. A car does an average of 15,000 km/year and therefore produces 2,250 kg of carbon dioxide/year.

2. GM IR cotton. Burkina Faso, India, Pakistan, Burma and China excluded because insecticides assumed to be applied by hand, using back pack sprayers.

The largest fuel use related reductions in carbon dioxide emissions have come from the adoption of GM HT technology in soybeans and how it has facilitated a switch to RT/NT production systems with their reduced soil cultivation practices (78% of total savings). These savings have been greatest in South America.

Over the period 1996 to 2013, the cumulative permanent reduction in fuel use has been about 18,722 million kg of carbon dioxide, arising from reduced fuel use of 7,012 million liters. In terms of car equivalents, this is equal to taking 8.32 million cars off the road for a year.

Additional soil carbon storage/sequestration

As indicated earlier, the widespread adoption and maintenance of RT/NT production systems in North and South America, facilitated by GM HT crops (especially in soybeans) has improved growers ability to control competing weeds, reducing the need to rely on soil cultivation and seed-bed preparation as means to getting good levels of weed control. As a result, as well as tractor fuel use for tillage being reduced, soil quality has been enhanced and levels of soil erosion cut. In turn, more carbon remains in the soil and this leads to lower GHG emissions.

Based on savings arising from the rapid adoption of RT/NT farming systems in North and South America, an extra 7,060 million kg of soil carbon is estimated to have been sequestered in 2013 (equivalent to 25,909 million kg of carbon dioxide that has not been released into the global atmosphere). These savings are equivalent to taking 11.5 million cars off the road for one year (Table 8).

TABLE 8. Context of carbon sequestration impact 2013 car equivalents

Crop/trait/country	Additional carbon stored in soil (million kg of carbon)	Potential additional soil carbon sequestration savings (million kg of carbon dioxide)	Soil carbon sequestration savings: as average family car equivalents removed from the road for a year (‘000s)
US: GM HT soybean	291	1,066	474
Argentina: GM HT soybean	3,111	11,418	5,075
Brazil GM HR soybean	1,889	6,931	3,081
Bolivia, Paraguay, Uruguay: GM HT soybean	700	2,569	1,142
US: GM HT maize	815	2,993	1,330
Canada: GM HT canola	254	932	414
Global GM IR cotton	0	0	0
Brazil IR maize	0	0	0
Total	7,060	25,909	11,515

The additional amount of soil carbon sequestered since 1996 has been equivalent to 203,560 million tonnes of carbon dioxide that has not been released into the global atmosphere. Readers should note that these estimates are based on fairly conservative assumptions and therefore the true values could be higher. Also, some of the additional soil carbon sequestration gains from RT/NT systems may be lost if subsequent ploughing of the land occurs.

Estimating the possible losses that may arise from subsequent ploughing would be complex and difficult to undertake. This factor should be taken into account when using the estimates presented in this paper. It should also be noted that this soil carbon saving is based on savings arising from the rapid adoption of RT/NT farming systems, for which the availability of GM HT technology, has been cited by many farmers as an important facilitator. GM HT technology has therefore probably been an important contributor to this increase in soil carbon sequestration, but is not the only factor of influence. Other influences such as the availability of relatively cheap generic glyphosate (the real price of glyphosate fell threefold between 1995 and 2000 once patent protection for the product expired) have also been important.

Cumulatively, the amount of carbon sequestered may be higher than these estimates due to year-on-year benefits to soil quality (e.g., less soil erosion, greater water retention and reduced levels of nutrient run off). However, it is equally likely that the total cumulative soil sequestration gains have been lower because only a proportion of the crop area will have remained in NT/RT.

It is, nevertheless, not possible to confidently estimate cumulative soil sequestration gains that take into account reversions to conventional tillage because of a lack of data. Consequently, the estimate provided of 229,374 million kg of carbon dioxide not released into the atmosphere should be treated with caution.

Aggregating the carbon sequestration benefits from reduced fuel use and additional soil carbon storage, the total carbon dioxide savings in 2013 are equal to about 28,005 million kg, equivalent to taking 12.4 million cars off the road for a year. This is roughly equal to 43% of registered cars in the UK.

Conclusions

During the last 18 years, the adoption of crop biotechnology by many farmers (18 million in 2013) has delivered important positive environmental contributions through its facilitation and evolution of environmentally friendly farming practices. More specifically:

• The environmental gains from the GM IR traits have mostly derived from decreased use of insecticides;

• The gains from GM HT traits have come from a combination of effects. In terms of the environmental impact associated with herbicide use, important changes in the profile of herbicides used have occurred, in favor of more environmentally benign products. Secondly, the technology has facilitated changes in farming systems, by enabling farmers to capitalise on the availability of a low cost, broad-spectrum herbicide (glyphosate) and move away from conventional to RT/NT production systems in both North and South America. This change in production system has reduced levels of GHG emissions from reduced tractor fuel use and additional soil carbon sequestration.

In relation to GM HT crops, however, over reliance on the use of glyphosate by some farmers, in some regions, has contributed to the development of weed resistance. As a result, farmers are increasingly adopting a mix of reactive and proactive weed management strategies incorporating a mix of herbicides and some of the original environmental gains associated with changes in herbicide use with GM HT crops have therefore diminished. Despite this, the adoption of GM HT crop technology continues to deliver a net environmental gain relative to the conventional alternative and, together with GM IR technology, continues to provide substantial net environmental benefits. These findings are also consistent with analysis by other authors (Klumper and Qaim, 2014, Fernandez-Cornejo et al., 2014).

Methodology

The available literature examining the environmental impact of pesticide use change and implications for greenhouse gas emissions associated with the adoption of GM crops is much more limited than the literature examining the economic impacts associated with use of the technology. Therefore while this analysis draws on the available literature, it includes a significant amount of ‘authors’ own analysis’ of farm level changes in husbandry practices and pesticide usage data. In particular, readers should note that the analysis of the environmental impact of pesticide usage changes with GM crops includes consideration of measures taken by some farmers to address issues of weed resistance to the main herbicide (glyphosate) used with GM HT crops.

Methodology: Environmental impacts from insecticide and herbicide use changes

Assessment of the impact of GM crops on insecticide and herbicide use requires comparisons of the respective weed and pest control measures used on GM versus the ‘conventional alternative’ form of production. This presents a number of challenges relating to availability and representativeness.

Comparison data ideally derives from farm level surveys which collect usage data on the different forms of production. A search of the literature on insecticide or herbicide use change with GM crops shows that the number of studies exploring these issues is limited (Qaim and De Janvry, 2005; Qaim and Traxler, 2002; Pray et al., 2002), with even fewer (Brookes, 2003, 2005), providing data to the pesticide (active ingredient) level. Secondly, national level pesticide usage survey data is also extremely limited; in fact there are no published, detailed, annual pesticide usage surveys conducted by national authorities in any of the countries currently growing GM crop traits and, the only country in which pesticide usage data is collected (by private market research companies) on an annual basis, and which allows a comparison between GM and conventional crops to be made, is the US. The US Department of Agriculture also conducts pesticide usage surveys but these are not conducted on an annual basis (e.g., the last time maize was included was 2014 and previous to this, in 2010 and 2005) and do not disaggregate usage by production type (GM vs. conventional).

Even where national pesticide use survey data is available, it is often of limited value. A reasonable estimate of the amount of herbicide or insecticide usage changes that have occurred with GM crop technology, requires an assessment of what herbicides/insecticides might reasonably be expected to be used in the absence of crop biotechnology on the relevant crops (ie, if the entire crops used non GM production methods). Applying usage rates for the current (remaining) conventional crops is one approach, however, this invariably provides significant under estimates of what usage might reasonably be in the absence of crop biotechnology, because the conventional cropping dataset used to identify pesticide use relates to a relatively small share of total crop area. This has been the case, for example, in respect of the US maize, canola, cotton and soybean crops for many years. Thus in 2013, the conventional share (not using GM HT technology) of each crop was only 7%, 10%, 10% and 7% respectively for soybean, maize, cotton and canola, with the conventional share having been below 50% of the total since 1999 in respect of the soybean crop, since 2001 for the cotton and canola crops, and since 2007 for the maize crop (source: USDA – note the conventional share refers to not using GM HT technology, with some of the ‘conventional crops’ using crop biotechnology-traited seed providing GM insect resistance only).

The reasons why this conventional cropping data set is unrepresentative of the levels of herbicide/insecticide use that might reasonably be expected in the absence of biotechnology include:

• While the degree of pest/weed problems/damage vary by year, region and within region, farmers’ who continue to farm conventionally may be those with relatively low levels of pest/weed problems, and hence see little, if any economic benefit from using the GM traits targeted at minimal pest/weed problems. In addition, late or non adopters of new technology in agriculture are typically those who generally make less use of newer technologies than earlier adopters. As a result, insecticide/herbicide usage levels for these non adopting farmers tends to be below the levels that would reasonably be expected on an average farm with more typical pest/weed infestations and where farmers are more wiling to adopt new technology;

• Some of the farms continuing to use conventional seed generally use extensive, low intensity production methods (including organic) which feature, limited (below average) use of herbicides/insecticides. The usage patterns of this sub-set of growers is therefore likely to understate usage for the majority of farmers if they all returned to farming without the use of GM technology;

• The widespread adoption of GM IR technology has resulted in ‘area-wide’ suppression of target pests in maize crops. As a result, conventional farmers (e.g., of maize in the US) have benefited from this lower level of pest infestation and the associated reduced need to conduct insecticide treatments (Hutchison et al., 2010);

• Some of the farmers using GM traits have experienced improvements in pest/weed control from using this technology relative to the conventional control methods previously used. If these farmers were to now switch back to using conventional techniques, it is likely that most would wish to maintain the levels of pest/weed control delivered with use of the GM traits and therefore some would use higher levels of insecticide/herbicide than they did in the pre GM crop days. This argument can, however, be countered by the constraining influence on farm level pesticide usage that comes from the cost of pesticides and their application. Ultimately the decision to use more pesticide or not would be made at the farm level according to individual assessment of the potential benefits (from higher yields) compared to the cost of additional pesticide use.

This problem of poor representativeness of the small conventional dataset has been addressed by firstly, using the average recorded values for insecticide/herbicide usage on conventional crops for years only when the conventional crop accounted for the majority of the total crop and, secondly, in other years (e.g., from 1999 for soybeans, from 2001 for cotton and from 2007 for maize in the US) applying estimates of the likely usage if the whole US crop was no longer using crop biotechnology, based on opinion from extension and industry advisors across the US as to what farmers might reasonably be expected to use in terms of weed control practices and usage levels of insecticide/herbicide. In addition, the usage levels identified from this methodology were cross checked (and subject to adjustment) against historic average usage levels of key herbicide and insecticide active ingredients from the private market research data set so as to minimise the scope for overstating likely usage levels on the conventional alternative. Overall, this approach has been applied in other countries where pesticide usage data is available, though more commonly, because of the paucity of available data, the analysis relies more on extension/advisor opinion and knowledge of actual and potential pesticide use.

This methodology has been used by others (Sankula and Blumenthal, 2003, 2006; Johnson and Strom, 2007). It also has the advantage of providing comparisons of current crop protection practices on both GM crops and the conventional alternatives and so takes into account dynamic changes in crop protection management practices and technologies rather than making comparisons solely on past practices. Details of how this methodology has been applied to the 2013 calculations, sources used for each trait/country combination examined and examples of typical conventional versus GM pesticide applications are provided in Appendices 1 and 2.

The most common way in which environmental impact associated with pesticide use changes with GM crops has typically been presented in the literature has been in terms of the volume (quantity) of pesticide applied. However, while the amount of pesticide applied to a crop is one way of trying to measure the environmental impact of pesticide use, this is not a good measure of environmental impact because the toxicity of each pesticide is not directly related to the amount (weight) applied. For example, the environmental impact of applying a kilogram of dioxin to a crop is far more toxic than applying a kilogram of salt. There exist alternative (and better) measures that have been used by a number of authors of peer reviewed papers to assess the environmental impact of pesticide use change with GM crops rather than simply looking at changes in the volume of active ingredient applied to crops. In particular, there are a number of peer reviewed papers that utilize the Environmental Impact Quotient (EIQ) developed at Cornell University by Kovach et al. (1992) and updated annually. This effectively integrates the various environmental impacts of individual pesticides into a single ‘field value per hectare’. The EIQ value is multiplied by the amount of pesticide active ingredient (ai) used per hectare to produce a field EIQ value. For example, the EIQ rating for glyphosate is 15.33. By using this rating multiplied by the amount of glyphosate used per hectare (e.g., a hypothetical example of 1.1 kg applied per ha), the field EIQ value for glyphosate would be equivalent to 16.86/ha. The EIQ indicator used is therefore a comparison of the field EIQ/ha for conventional vs. GM crop production systems, with the total environmental impact or load of each system, a direct function of respective field EIQ/ha values and the area planted to each type of production (GM versus conventional). The use of environmental indicators is commonly used by researchers and the EIQ indicator has been, for example, cited by Brimner et al. (2005) in a study comparing the environmental impacts of GM and conventional canola and by Kleiter (2005). The EIQ indicator provides an improved assessment of the impact of GM crops on the environment when compared to only examining changes in volume of active ingredient applied, because it draws on some of the key toxicity and environmental exposure data related to individual products, as applicable to impacts on farm workers, consumers and ecology.

The authors of this analysis have also used the EIQ indicator now for several years because it:

• Summarizes significant amounts of information on pesticide impact into a single value that, with data on usage rates (amount of active used per hectare) can be readily used to make comparisons between different production systems across many regions and countries;

• Provides an improved assessment of the impact of GM crops on the environment when compared to only examining changes in volume of active ingredient applied, because it draws on some of the key toxicity and environmental exposure data related to individual products, as applicable to impacts on farm workers, consumers and ecology.

The authors, do, however acknowledge that the EIQ is only a hazard indicator and has important weaknesses (see for example, Peterson and Schleier, 2014). It is a hazard rating indicator that does not assess risk or probability of exposure to pesticides. It also relies on qualitative assumptions for the scaling and weighting of (quantitative) risk information that can result, for example, in a low risk rating for one factor (e.g., impact on farm workers) may cancel out a high risk rating factor for another factor (e.g., impact on ecology). Fundamentally, assessing the full environmental impact of pesticide use changes with different production systems is complex and requires an evaluation of risk exposure to pesticides at a site specific level. This requires substantial collection of (site-specific) data (e.g., on ground water levels, soil structure) and/or the application of standard scenario models for exposure in a number of locations. Undertaking such an exercise at a global level would require a substantial and ongoing input of labor and time, if comprehensive environmental impact of pesticide change analysis is to be completed. It is not surprising that no such exercise has, to date been undertaken, or likely to be in the near future.

Despite the acknowledged weaknesses of the EIQ as an indicator of pesticide environmental impact, the authors of this paper continue to use the EIQ as an indicator of the environmental impact of pesticide use change with GM crops because it is, in our view, a superior indicator to only using amount of pesticide active ingredient applied. In this paper, the EIQ indicator is used in conjunction with examining changes in the volume of pesticide active ingredient applied.

Detailed examples of the relevant amounts of active ingredient used and their associated field EIQ values for GM vs. conventional crops for the year 2013 are presented in Appendix 2.

Methodology: Impact of greenhouse gas emissions

The methodology used to assess impact on greenhouse gas emissions combines reviews of literature relating to changes in fuel and tillage systems and carbon emissions, coupled with evidence from the development of relevant GM crops and their impact on both fuel use and tillage systems. Reductions in the level of GHG emissions associated with the adoption of GM crops are acknowledged in a wide body of literature (American Soybean Association, 2001; CTIC, 2002; Derpsch et al., 2010; Eagle et al., 2012; Fabrizzi et al., 2003; Jasa, 2002; Johnson et al., 2005; Leibig et al., 2005; Olson et al., 2013; Reicosky, 1995; Robertson et al., 2000; West and Post, 2002).

First, GM crops contribute to a reduction in fuel use due to less frequent herbicide or insecticide applications and a reduction in the energy use in soil cultivation. For both herbicide and insecticide spray applications, the quantity of energy required to apply the pesticides depends upon the application method. For example, in the USA, a typical method of application is with a 50 foot boom sprayer which consumes approximately 0.84 liters/ha (Lazarus, 2013). In terms of GHG, each liter of tractor diesel consumed contributes an estimated 2.67 kg of carbon dioxide into the atmosphere (this also updates a previously used co-efficient of 2.75 to convert 1 liter of diesel to kg of carbon dioxide). Given that many farmers apply insecticides via sprayers pulled by tractors, which tend to use higher levels of fuel than self-propelled boom sprayers, these estimates for reductions in carbon emissions, which are based on self-propelled boom application, probably understate the carbon benefits.

In addition, there has been a shift from conventional tillage (CT) to reduced/no till (RT/NT). No-till farming means that the ground is not ploughed at all, while reduced tillage means that the ground is disturbed less than it would be with traditional tillage systems. For example, under a no-till farming system, soybean seeds are planted through the organic material that is left over from a previous crop such as corn, cotton or wheat) facilitated by GM HT technology (see for example, CTIC, 2002; American Soybean Association, 2001), especially where soybean growing and/or a soybean: corn rotation are commonplace. Before the introduction of GM HT technology, RT/NT systems were practised by some farmers with varying degrees of success using a number of herbicides, though in many cases, a reversion to CT was common after a few years due to poor levels of weed control. The availability of GM HT technology provided growers with an opportunity to control weeds in a RT/NT system with a non residual, broad-spectrum, foliar herbicide as a ‘burndown’ pre-seeding treatment followed by a post-emergent treatment when the crop became established, in what proved to be a more reliable and commercially attractive system than was previously possible. These technical and cost advantages have contributed to the rapid adoption of GM HT cultivars and RT/NT production systems. For example, there has been a 50% increase in the RT/NT soybean area in the US and a 7-fold increase in Argentina since 1996. In 2013, RT/NT production accounted for 80% and 89% respectively of total soybean production in the US and Argentina, with over 95% of the RT/NT soybean crop area in both countries using GM HT technology.

Substantial growth in RT/NT production systems have also occurred in Canada, where the proportion of the total canola crop accounted for by RT/NT systems increased from 25% in 1996 to 50% by 2004, and in 2013, accounted for 75% of the total crop (95% the RT/NT canola area is planted with GM HT cultivars).

This shift away from a plough-based, to a RT/NT production system has resulted in a reduction in fuel use. The fuel savings used in this paper are drawn from a review of literature including CTIC, 2002; Jasa, 2002; Reeder, 2010; University of Illinois, 2006; USDA Energy Estimator, 2013; USDA Comet-VR, 2013. In the analysis presented below, it is assumed that the adoption of NT farming systems in soybean production reduces cultivation and seedbed preparation fuel usage by 27.12 liters/ha compared with traditional conventional tillage and in the case of RT (mulch till) cultivation by 10.39 liters/ha. In the case of maize, NT results in a saving of 24.41 liters/ha and 7.52 liters/ha in the case of RT compared with conventional intensive tillage. These are conservative estimates and are in line with the USDA Energy Estimator for soybeans and maize.

The adoption of NT and RT systems in respect of fuel use therefore results in reductions of carbon dioxide emissions of 72.41 kg/ha and 27.74 kg/ha respectively for soybeans and 65.17 kg/ha and 20.08 kg/ha for maize.

Secondly, the use of RT/NT farming systems increases the amount of organic carbon in the form of crop residue that is stored or sequestered in the soil and therefore reduces carbon dioxide emissions to the environment. A number of researchers have examined the relationship between carbon sequestration and different tillage systems (Angers and Eriksen-Hamel, 2008; Baker et al., 2007; Bernacchi et al., 2005; Blanco-Canqui and Lal, 2008; Calegari et al., 2002; Intergovernmental Panel on Climate Change, 2006; Johnson et al., 2005; Lal, 2004, 2005, 2010; Leibig et al., 2005; Michigan State University, 2013; Robertson et al., 2000). This literature shows that the amount of carbon sequestered varies by soil type, cropping system, eco-region and tillage depth. It also shows that tillage systems can impact on levels of other GHG emissions such as methane and nitrous oxide and on crop yield.

Overall, the literature highlights the difficulty in estimating the contribution NT/RT systems can make to soil carbon sequestration, especially because of the dynamic nature of soils, climate, cropping types and patterns. If a specific crop area is in continuous NT crop rotation, the full soil carbon sequestration benefits described in the literature can be realized. However, if the NT crop area is returned to a conventional tillage system, a proportion of the soil organic carbon gain will be lost. The temporary nature of this form of carbon storage only becomes permanent when farmers adopt a continuous NT system, which as indicated earlier, is highly dependent upon having an effective herbicide-based weed control systems.

Estimating long-term soil carbon sequestration is also further complicated by the hypothesis typically used in soil carbon models that the level of soil organic carbon (SOC) reaches an equilibrium when the amount of carbon stored in the soil equals the amount of carbon released (the Carbon-Stock Equilibrium (CSE)). This implies that as equilibrium is reached the rate of soil carbon sequestration may decline and therefore if equilibrium is being reached after many years of land being in NT, the rate of carbon sequestration in GM HT may be declining. Our estimates presented in this paper, however, assume that a constant rate of carbon sequestration occurs because of the relatively short time period that NT/RT production systems have been operated (and hence the time period that land may have been in ‘permanent non cultivation is a maximum of 15–20 years). In addition, some researchers question whether the CSE assumption that is used in most soil models is valid because of the scope for very old soils to continue to store carbon (Lal, 2005).

Drawing on the literature and models referred to above, the analysis presented in the following sub-sections assumes the following:

US: In previous editions of this report no differentiation was made between corn and soybeans and the assumptions used were based on a difference between NT and CT of 400 kg of carbon/ha/year of soil carbon sequestered (NT systems store 375 kg of carbon/ha/year; RT systems store 175 kg of carbon/ha/year; and CT systems store 25 kg of carbon/ha/year). In this report, the soil carbon sequestered by tillage system for corn in continuous rotation with soybeans is assumed to be a net sink of 250 kg of carbon/ha/year based on:

• NT systems store 251 kg of carbon/ha/year;

• RT systems store 75 kg of carbon/ha/year;

• CT systems store 1 kg of carbon/ha/year.

The soil carbon sequestered by tillage system for soybeans in a continuous rotation with corn is assumed to be a net sink of 100 kg of carbon/ha/year based on:

• NT systems release 45 kg of carbon/ha/year;

• RT systems release 115 kg of carbon/ha/year;

• CT systems release 145 kg of carbon/ha/year.

Argentina and Brazil: soil carbon retention is 275 kg carbon/ha/year for NT soybean cropping and CT systems release 25 kg carbon/ha/year (a difference of 300 kg carbon/ha/year). In previous editions of this report the difference used was 200 kg carbon/ha/year.

Overall, the GHG emission savings derived from reductions in fuel use for crop spraying have been applied only to the area of GM IR crops worldwide (but excluding countries where conventional spraying has traditionally been by hand, such as in India and China) and the savings associated with reductions in fuel from less soil cultivation plus soil carbon storage have been limited to NT/RT areas in North and South America that have utilised GM HT technology. Lastly, some RT/NT areas have also been excluded where the consensus view is that GM HT technology has not been the primary reason for use of these non plough-based systems (ie, parts of Brazil).

Additional detail relating to the estimates for carbon dioxide savings at the country and trait levels are presented in Appendix 3.

Disclosure of Potential Conflicts of Interest

No potential conflicts of interest were disclosed.

Funding

The authors acknowledge that funding toward the researching of this paper was provided by Monsanto. The material presented in this paper is, however, the independent views of the authors—it is a standard condition for all work undertaken by PG Economics that all reports are independently and objectively compiled without influence from funding sponsors.

Appendix 1: Details of Methodology as Applied to 2012 Calculations of Environmental Impact Associated with Pesticide Use Changes

GM IR maize (targeting stalk boring pests) 2013

Table

Country	Area of trait (‘000 ha)	Maximum area treated for stalk boring pests: pre GM IR (‘000 ha)	Average ai use GM crop (kg/ha)	Average ai use if conventional (kg/ha)	Average field EIQ/ha GM crop	Average field EIQ/ha if conventional	Aggregate change in ai use (‘000 kg)	Aggregate change in field EIQ/ha units (millions)
US	26,964	3,548	0.23	0.58	12.8	32.8	−1,242	−35.5
Canada	1,243	74	0.04	0.64	4.8	24.8	−44	−1.2
Argentina	3,423	0	0	0	0	0	0	0
Philippines	715	Very low – assumed zero	0	0	0	0	0	0
South Africa	2,719	1,768	0	0.09	0	3.2	−165	−6.0
Spain	137	43.9	0.36	1.32	0.9	26.9	−42	−1.1
Uruguay	107	Assumed to be zero: as Argentina	0	0	0	0	0	0
Brazil	11,880	7,330	0 targeted at stalk boring pests	0.36 targeted at stalk boring pests	0 targeting stalk boring pests	21.5	−2,609	−157
Colombia	65.9	48.5	0 targeted at stalk boring pests	0.56 targeted at stalk boring pests	0 targeting stalk boring pests	15.9	−27	−0.77

Note:

1. Other countries: Honduras and EU countries: not examined due to lack of data (Honduras) or very small area planted (EU countries other than Spain).

2. Baseline amount of insecticide active ingredient shown in Canada refers only to insecticides used primarily to control stalk boring pests.

GM IR maize (targeting rootworm) 2013

Table

Country	Area of trait (‘000 ha)	Maximum area treated for rootworm pests: pre GM IR (‘000 ha)	Average ai use GM crop (kg/ha)	Average ai use if conventional (kg/ha)	Average field EIQ/ha GM crop	Average field EIQ/ha if conventional	Aggregate change in ai use (‘000 kg)	Aggregate change in field EIQ/ha units (millions)
US	18,942	10,284	0.2	0.6	12	32.5	−4,113	−210.8

Note:

1. There are no Canadian-specific data available: analysis has therefore not been included for the Canadian crop of 835,000 ha planted to seed containing GM IR traits targeted at rootworm pests.

2. The maximum area treated for corn rootworm (on which the insecticide use change is based) is based on the historic area treated with insecticides targeted at the corn rootworm. This is 30% of the total crop area. The 2013 maximum area on which this calculation is made has, however been reduced by 1 million ha in 2013 to reflect the increased use of soil based insecticides (relative to usage in a baseline period of 2008−2010) that target the corn rootworm on the GM IR (targeting corn rootworm) area. It is assumed this increase in usage is in response to farmer concerns about the possible development of CRW resistance to the GM IR rootworm technology that has been reported in a small area in the US.

GM IR cotton 2013

Table

Country	Area of trait (‘000 ha)	Average ai use GM crop (kg/ha)	Average ai use if conventional (kg/ha)	Average field EIQ/ha GM crop	Average field EIQ/ha if conventional	Aggregate change in ai use (‘000 kg)	Aggregate change in field EIQ/ha units (millions)
US	2,296	0.73	1.59	22.2	40.5	−1,968	−42.0
China	4,200	2.10	3.48	87.0	122.5	−5,767	−149.3
Australia	399	0.91	2.1	25.0	65.0	−474	−16.0
Mexico	100	3.60	5.22	120.4	177.0	−162	−5.7
Argentina	484	0.7	2.42	19.9	76.7	−835	−27.5
India	11,000	0.63	1.77	18.8	74.8	−11,905	−615.8
Brazil	440

Note:

1. Due to the widespread and regular nature of bollworm and budworm pest problems in cotton crops, GM IR areas planted are assumed to be equal to the area traditionally receiving some form of conventional insecticide treatment.

2. South Africa, Burkina Faso, Columbia, Pakistan and Burma not included in analysis due to lack of data on insecticide use changes.

3. Brazil: due to a lack of data, usage patterns from Argentina have been assumed.

GM HT soybean 2013

Table

Country	Area of trait (‘000 ha)	Average ai use GM crop (kg/ha)	Average ai use if conventional (kg/ha)	Average field EIQ/ha GM crop	Average field EIQ/ha if conventional	Aggregate change in ai use (‘000 kg)	Aggregate change in field EIQ/ha units (millions)
US	28,544	2.17	2.21	36.4	41.5	−1,142	−147.1
Canada	1,442	1.32	1.43	20.9	34.2	+156	−18.9
Argentina	19,651	3.11	2.82	48.2	48.7	+5,754	−9.9
Brazil	27,800	2.59	2.53	40.6	47.4	+1,716	−188.1
Paraguay	2,945	3.18	3.03	50.6	51.8	+441	−3.6
South Africa	463	1.08	1.46	16.6	27.11	−175	−4.9
Uruguay	1,443	2.98	2.82	47.5	48.7	+236	−1.8
Bolivia	1,001	3.18	3.03	50.6	51.8	+150	+1.2
Mexico	12	1.62	1.76	24.8	41.0	−2	−0.2

Note: Due to lack of country-specific data, usage patterns in Paraguay assumed for Bolivia and usage patterns in Argentina assumed for Uruguay. Industry sources confirm this assumption reasonably reflects typical usage.

GM IR (Intacta) soybeans 2013

Table

Country	Area of trait (‘000 ha)	Average ai use GM crop (kg/ha)	Average ai use if conventional (kg/ha)	Average field EIQ/ha GM crop	Average field EIQ/ha if conventional	Aggregate change in ai use (‘000 kg)	Aggregate change in field EIQ/ha units (millions)
Brazil	2,359	1.43	1.6	30.65	47.9	−401	−40

Note:

There are also small areas of Intacta in Argentina, Paraguay and Uruguay in 2013. No country-specific data on insecticide use and how Intacta has impacted on this available at time of analysis in early 2015.

GM HT maize 2013

Table

Country	Area of trait (‘000 ha)	Average ai use GM crop (kg/ha)	Average ai use if conventional (kg/ha)	Average field EIQ/ha GM crop	Average field EIQ/ha if conventional	Aggregate change in ai use (‘000 kg)	Aggregate change in field EIQ/ha units (millions)
US glyphosate tolerant	29,855	3.01	3.7	57.3	73.8	−20,600	−492.3
US glufosinate tolerant	301	3.01	3.7	57.3	73.8	−208	−5
Canada glyphosate tolerant	1,414	1.83	2.71	37.0	61.1	−1,245	−34.2
Canada glufosinate tolerant	14	1.64	2.71	36.0	61.0	−15	−0.4
Argentina	2,769	3.99	3.53	71.8	73.6	+1,284	−5.0
South Africa	1,690	2.85	3.15	53.7	66.1	−507	−21
Brazil	6,291	3.91	3.99	70.3	86.1	−515	−100
Uruguay	120	3.99	3.53	71.8	73.6	+44	−0.2

Note:

One. Philippines: not included due to lack of data on weed control methods and herbicide product use.

Two. Uruguay – based on Argentine data – industry sources confirm herbicide use in Uruguay is very similar.

GM HT cotton 2013

Table

Country	Area of trait (‘000 ha)	Average ai use GM crop (kg/ha)	Average ai use if conventional (kg/ha)	Average field EIQ/ha GM crop	Average field EIQ/ha if conventional	Aggregate change in ai use (‘000 kg)	Aggregate change in field EIQ/ha units (millions)
US	2,510	4.21	4.96	76.3	95.3	−1,893	−47.9
S Africa	8	1.80	1.81	27.6	31.9	−0.1	−0.03
Australia	417	3.10	4.76	51.7	87.5	−694	−14.9
Argentina	550	4.06	4.72	64.0	78.4	−361	−7.9

Note:

1. Mexico and Colombia: not included due to lack of data on herbicide use.

GM HT canola 2013

Table

Country	Area of trait (‘000 ha)	Average ai use GM crop (kg/ha)	Average ai use if conventional (kg/ha)	Average field EIQ/ha GM crop	Average field EIQ/ha if conventional	Aggregate change in ai use (‘000 kg)	Aggregate change in field EIQ/ha units (millions)
US glyphosate tolerant	203	1.16	1.06	17.7	22.9	+20	−1.1
US glufosinate tolerant	194	0.44	1.06	8.8	22.9	−122	−2.7
Canada glyphosate tolerant	3,891	1.16	1.06	17.7	22.9	+371	−20.0
Canada glufosinate tolerant	3,555	0.44	1.06	8.8	22.9	−2,227	−50.1
Australia glyphosate tolerant	222	0.52	14	7.3	22.3	−117	−1.6

GM herbicide tolerant sugar beet 2013

Table

Country	Area of trait (‘000 ha)	Average ai use GM crop (kg/ha)	Average ai use if conventional (kg/ha)	Average field EIQ/HA GM crop	Average field EIQ/ha if conventional	Aggregate change in ai use (‘000 kg)	Aggregate change in field EIQ/ha units
US	458	2.4	1.6	36.8	34.5	+367	+1.0

Appendix 2: Examples of Eiq Calculations

Estimated typical herbicide regimes for GM HT reduced/no till and conventional reduced/no till soybean production systems that will provide an equal level of weed control to the GM HT system in Argentina 2013

Table

	Active ingredient (kg/ha)	Field EIQ/ha value
GM HT soybean	3.11	48.2
Source: AMIS Global dataset on pesticide use 2013
Conventional soybean
Option 1
Glyphosate	1.62	24.83
Metsulfuron	0.03	0.50
2 4 D	0.30	6.21
Imazethapyr	0.10	1.96
Diflufenican	0.03	0.29
Clethodim	0.19	3.23
Total	2.27	37.03
Option 2
Glyphosate	1.62	24.83
Dicamba	0.12	3.04
Acetochlor	1.08	21.49
Haloxifop	0.12	2.66
Sulfentrazone	0.19	2.23
Total	3.13	54.25
Option 3
Glyphosate	1.62	24.83
Atrazine	0.87	19.92
Bentazon	0.60	11.22
2 4 D ester	0.04	0.61
Imazaquin	0.024	0.37
Total	3.154	56.96
Option 4
Glyphosate	1.8	27.59
2 4 D amine	0.384	7.95
Flumetsulam	0.06	0.94
Fomesafen	0.25	0.13
Chlorimuron	0.01	0.29
Fluazifop	0.12	3.44
Total	2.63	46.34
Option 5
Glyphosate	1.8	27.59
Metsulfuron	0.03	0.50
2 4 D amine	0.75	15.53
Imazethapyr	0.1	1.96
Haloxifop	0.12	2.66
Total	2.80	48.24
Option 6
Glyphosate	1.8	27.59
Metsulfuron	0.03	0.50
2 4 D amine	0.75	15.53
Imazethapyr	0.1	1.96
Clethodim	0.24	4.08
Total	2.92	49.66
Average all 6 conventional options	2.82	48.75

Sources: AAPRESID 2014, AMIS Global 2014, Monsanto Argentina 2014.

Typical insecticide regimes for cotton in India 2013

Table

Active ingredient	Amount (kg/ha of crop)	Field EIQ/ha
Conventional cotton Option 1
Imidacloprid	0.06	2.2
Thiomethoxam	0.05	1.67
Acetamiprid	0.05	1.45
Diafenthiuron	0.1	2.53
Buprofezin	0.07	2.55
Profenfos	0.81	48.28
Acephate	0.63	15.79
Cypermethrin	0.1	3.64
Metaflumizone	0.03	0.82
Novaluron	0.04	0.57
Total	1.94	79.5
Option 2
Imidacloprid	0.06	2.2
Thiomethoxam	0.05	1.67
Acetamiprid	0.05	1.45
Diafenthiuron	0.1	2.53
Chloripyrifos	0.39	10.58
Profenfos	0.81	48.28
Metaflumizone	0.03	0.82
Emamectin	0.01	0.29
Total	1.50	67.83
Average conventional	1.73	73.67
GM IR cotton
Imidacloprid	0.06	2.2
Thiomethoxam	0.05	1.67
Acetamiprid	0.05	1.45
Diafenthiuron	0.1	2.53
Buprofezin	0.07	2.55
Acephate	0.63	15.79
Total	0.97	26.19
Option 2
Imidacloprid	0.06	1.54
Thiomethoxam	0.05	1.67
Acetamiprid	0.05	2.30
Diafenthiuron	0.1	2.53
Total	0.26	8.04
Weighted average GM IR cotton	0.68	18.85

Sources: Monsanto India 2014, AMIS Global 2014.

Note weighted average for GM IR cotton based on insecticide usage – option 1 60%, option 2 40%.

Data sources (for pesticide usage data)

Table

	Sources of data for assumptions
US	Gianessi and Carpenter (1999) (Sankula and Blumenthal, 2003, 2006) Johnson and Strom (2007) Own analysis (2010–2013) All of the above mainly for conventional regimes (based on surveys and consultations of extension advisors and industry experts) GFK Plant and Animal Health – private market research data on pesticide usage. Is the most comprehensive data set on crop pesticide usage at the farm level and allows for disaggregation to cover biotech versus conventional crops. This source primarily used for usage on GM traits
Argentina	AMIS Global & Kleffmann - private market research data on pesticide use. Is the most detailed dataset on crop pesticide use AAPRESID (farmer producers association) – personal communications 2007 Monsanto Argentina (personal communications 2005, 2007, 2009, 2010, 2011, 2012, 2013, 2014) Qaim and De Janvry (2005) Qaim and Traxler (2002)
Brazil	AMIS Global & Kleffmann - private market research data on crop pesticide use. Is the most detailed data set on crop pesticide use Monsanto Brazil (2008) (Galveo, 2009, 2012), plus personal communications Monsanto Brazil (personal communications 2007, 2009, 2011, 2013, 2014)
Uruguay	AMIS Global and as Argentina for conventional
Paraguay	As Argentina for conventional soybeans (over the top usage), AMIS Global for GM HT soybean
Bolivia	As Paraguay: no country-specific data identified
Canada	George Morris Centre (2004) Canola Council of Canada (2001) Smyth et al. (2011) Weed Control Guide Ontario (updated annually)
S Africa	Monsanto S Africa (personal communications 2005, 2007, 2009, 2010, 2011, 2012, 2014)
	Ismael et al. (2002) AMIS Global
Romania	AMIS Global, Brookes (2005)
Australia	AMIS Global (Doyle, 2005, 2003) CSIRO (2005) Monsanto Australia (personal communications 2005, 2007, 2009, 2010, 2011, 2012, 2014) Fisher and Tozer (2009)
Spain	(Brookes, 2003, 2008)
China	AMIS Global Pray et al. (2002) Monsanto China personal communication (2007, 2009, 2010, 2011, 2013, 2014)
Mexico	(Monsanto Comercial Mexico, 2013, 2012, 2009, 2008, 2007, 2005) Traxler et al. (2001)
India	AMIS Global APCOAB (2006) (IMRB, 2006, 2007) Monsanto India (2007, 2008, 2009, 2010, 2011, 2013) – personal communications

Appendix 3: Carbon Saving Estimates: Additional Information

US soybean: Permanent reduction in tractor fuel consumption and reduction in carbon dioxide emissions (1996–2013)

Table

	Annual reduction based on 1996 average (liters/ha)	Crop area (million ha)	Total fuel saving (million liters)	Carbon dioxide (million kg)
1996	0.0	26.0	0.0	0.0
1997	0.4	28.3	11.4	30.3
1998	0.8	29.2	23.4	62.4
1999	0.9	29.8	25.6	68.5
2000	0.9	30.1	27.7	73.9
2001	1.2	30.0	34.9	93.3
2002	1.4	29.5	41.7	111.4
2003	1.9	29.7	56.6	151.2
2004	2.4	30.8	72.7	194.1
2005	2.5	28.0	73.3	195.8
2006	2.7	30.6	81.8	218.5
2007	2.9	25.7	75.8	202.5
2008	2.9	30.2	87.3	233.2
2009	2.9	30.9	89.3	238.6
2010	2.7	31.6	86.0	229.6
2011	2.6	30.0	76.9	205.2
2012	2.6	30.8	78.8	210.3
2013	2.6	30.7	78.5	209.7
Total			1,022.1	2,728.5

Assumption: baseline fuel usage is the 1996 level of 36.6 liters/ha.

Note: Due to rounding the cumulative totals may not exactly sum the annual totals. This applies to all tables in this appendix.

US soybean: Potential additional soil carbon sequestration (1996 to 2012)

Table

	Annual increase in carbon sequestered based on 1996 average (kg carbon/ha)	Crop area (million ha)	Total additional carbon sequestered (million kg)	Total additional Carbon dioxide sequestered (million kg)
1996	0.0	26.0	0.00	0.00
1997	1.4	28.3	39.4	144.3
1998	2.8	29.1	80.9	297.0
1999	3.1	29.8	91.0	334.1
2000	3.3	30.1	100.2	367.8
2001	4.3	30.0	127.8	469.0
2002	5.2	29.5	153.6	563.6
2003	7.0	29.7	208.8	766.3
2004	8.9	30.3	268.2	984.3
2005	9.5	28.9	272.9	1,001.6
2006	10.0	30.6	306.9	1,126.4
2007	11.1	25.8	284.8	1,045.3
2008	10.9	30.2	327.9	1,203.5
2009	10.9	30.9	335.5	1,231.5
2010	10.2	31.6	320.7	1,177.0
2011	9.5	30.1	284.3	1,043.6
2012	9.5	30.8	291.4	1,069.6
2013	9.5	30.7	290.5	1,066.2
Total			3,784.8	13,890.8

Assumption: carbon sequestration remains at the 1996 level of −102.9 kg carbon/ha/year.

Argentine soybean: Permanent reduction in tractor fuel consumption and reduction in carbon dioxide emissions (1996–2013)

Table

	Annual reduction based on 1996 average of 39.1 (liters/ha)	Crop area (million ha)	Total fuel saving (million liters)	Carbon dioxide (million kg)
1996	0.0	5.9	0.0	0.0
1997	2.3	6.4	14.7	39.2
1998	3.1	7.0	21.5	57.4
1999	2.7	8.2	21.9	58.5
2000	3.0	10.6	31.6	84.4
2001	5.8	11.5	67.2	179.4
2002	8.3	13.0	107.3	286.6
2003	9.8	13.5	132.2	352.9
2004	11.7	14.3	167.4	447.0
2005	10.7	15.2	163.0	435.2
2006	11.0	16.2	177.4	473.7
2007	12.3	16.6	204.2	545.1
2008	13.7	16.8	230.4	615.1
2009	13.2	18.6	245.9	656.5
2010	13.7	18.2	249.8	667.1
2011	14.2	18.6	261.6	698.5
2012	14.2	19.4	264.9	707.2
2013	14.2	19.8	281.3	751.0
Total			2,656.3	7,092.2

Note: based on 21.89 liters/ha for NT and 49.01 liters/ha for CT.

Argentine soybean: Potential additional soil carbon sequestration (1996 to 2013)

Table

	Annual increase in carbon sequestered based on 1996 average (kg carbon/ha)	Crop area (million ha)	Total additional carbon sequestered (million kg)	Total additional Carbon dioxide sequestered (million kg)
1996	0.0	5.9	0.0	0.00
1997	25.4	6.4	162.1	595.5
1998	34.2	7.0	237.8	872.7
1999	29.6	8.2	242.5	890.1
2000	33.0	10.6	349.9	1,284.1
2001	64.6	11.5	743.3	2,727.9
2002	91.6	13.0	1,187.3	4,357.2
2003	108.3	13.5	1,462.1	5,365.8
2004	129.1	14.3	1,852.0	6,797.0
2005	118.6	15.2	1,803.0	6,617.0
2006	121.5	16.2	1,962.7	7,203.2
2007	136.2	16.6	2,258.6	8,289.0
2008	152.0	16.8	2,548.5	9,353.0
2009	146.2	18.6	2,720.1	9,982.6
2010	151.9	18.2	2,763.7	10,142.7
2011	157.5	18.6	2,930.1	10,753.3
2012	157.5	19.4	3,048.2	11,186.9
2013	157.5	19.8	3,111.2	11,418.1
Total			29,383.1	107,836.1

Assumption: NT = +275 kg carbon/ha/yr, CT = −25 kg carbon/ha/yr.

Brazil (3 southernmost states) soybean: Permanent reduction in tractor fuel consumption and reduction in carbon dioxide emissions (1997–2013)

Table

	Annual reduction based on 1997 average of 40.8 (liters/ha)	Crop area (million ha)	Total fuel saving (million liters)	Carbon dioxide (million kg)
1997	0.0	6.2	0.0	0.0
1998	1.4	6.1	8.3	22.1
1999	2.7	6.1	16.4	43.8
2000	4.1	6.0	24.3	65.0
2001	5.4	6.8	37.1	99.0
2002	6.8	7.5	50.8	135.5
2003	8.1	8.2	66.8	178.4
2004	9.5	8.6	81.5	217.6
2005	10.8	8.3	90.0	240.3
2006	12.2	8.2	100.6	268.7
2007	12.2	8.2	100.0	266.7
2008	13.6	8.2	111.6	297.9
2009	14.4	8.9	127.9	341.6
2010	14.9	9.1	136.2	363.7
2011	14.9	9.1	135.8	362.6
2012	15.5	9.9	152.8	408.0
2013	16.3	10.5	170.7	455.9
Total			1,410.8	3,761.1

Note: based on 21.89 liters/ha for NT and RT and 49.01 liters/ha for CT.

Brazil (3 southernmost states) soybean: Potential additional soil carbon sequestration (1997 to 2013)

Table

	Annual increase in carbon sequestered based on 1997 average (kg carbon/ha)	Crop area (million ha)	Total addition carbon sequestered (million kg)	Total addition Carbon dioxide sequestered (million kg)
1997	0.0	6.2	0.0	0.0
1998	15.0	6.1	91.8	336.8
1999	30.0	6.0	181.5	666.0
2000	45.0	6.0	269.3	988.3
2001	60.0	6.8	410.3	1,505.7
2002	75.0	7.5	561.5	2,060.8
2003	90.0	8.2	739.3	2,713.1
2004	105.0	8.6	901.7	3,309.4
2005	120.0	8.3	995.4	3,653.1
2006	135.0	8.2	1,113.3	4,086.0
2007	135.0	8.2	1,105.0	4,055.3
2008	150.0	8.2	1,234.0	4,529.0
2009	159.0	8.9	1,415.3	5,194.0
2010	165.0	9.1	1,507.0	5,530.8
2011	165.0	9.1	1,502.5	5,514.2
2012	171.0	9.9	1,690.1	6,202.8
2013	180.0	10.5	1,888.7	6,931.5
Total			13,658.9	57,276.8

Assumption: NT/RT = +275 kg carbon/ha/yr, CT = −25 kg carbon/ha/yr.

US maize: Permanent reduction in tractor fuel consumption and reduction in carbon dioxide emissions (1998–2013)

Table

	Annual reduction based on 1998 average (liters/ha)	Crop area (million ha)	Total fuel saving (million liters)	Carbon dioxide (million kg)
1998	0.0	32.4	0.0	0.0
1999	0.1	31.3	4.8	12.8
2000	0.3	32.2	9.9	26.4
2001	0.4	30.6	12.3	33.0
2002	0.5	31.9	15.9	42.5
2003	0.6	31.8	19.4	51.8
2004	0.7	32.5	23.4	62.5
2005	1.1	33.1	35.6	95.1
2006	1.4	31.7	45.4	121.2
2007	1.8	37.9	67.0	178.9
2008	1.6	31.8	52.1	139.1
2009	2.3	32.2	73.2	195.6
2010	2.1	32.8	67.9	181.2
2011	2.2	34.3	76.4	203.9
2012	2.2	35.4	78.6	209.9
2013	2.2	35.5	78.9	210.6
Total			660.8	1,764.4

Assumption: baseline fuel usage is the 1998 level of 46.9 liters/ha.

US maize: Potential additional soil carbon sequestration (1998 to 2013)

Table

	Annual increase in carbon sequestered based on 1998 average (kg carbon/ha)	Crop area (million ha)	Additional carbon sequestered (million kg)	Additional Carbon dioxide sequestered (million kg)
1998	0.0	32.4	0.0	0.0
1999	1.6	31.3	51.4	188.8
2000	3.3	32.2	105.8	388.3
2001	4.3	30.6	130.8	479.9
2002	5.3	31.9	167.6	615.2
2003	6.4	31.8	203.3	746.3
2004	7.5	32.5	244.6	897.8
2005	11.2	33.1	370.3	1,359.2
2006	14.8	31.7	470.6	1,727.0
2007	18.3	37.9	691.3	2,537.1
2008	16.9	31.8	537.4	1,972.4
2009	23.5	32.2	756.8	2,777.3
2010	21.4	32.8	701.8	2,575.6
2011	23.0	34.4	789.6	2,897.7
2012	23.0	35.4	812.7	2,982.6
2013	23.0	35.5	815.4	2,992.6
Total			6,849.4	25,138.8

Assumption: carbon sequestration remains at the 1998 level of 77.3 kg carbon/ha/year.

Canadian canola: Permanent reduction in tractor fuel consumption and reduction in carbon dioxide emissions (1996–2013)

Table

	Annual reduction based on 1996 average 30.6 (l/ha)	Crop area (million ha)	Total fuel saving (million liters)	Carbon dioxide (million kg)
1996	0.0	3.5	0.0	0.0
1997	0.9	4.9	4.3	11.5
1998	0.9	5.4	4.8	12.8
1999	0.9	5.6	4.9	13.1
2000	0.9	4.9	4.3	11.5
2001	1.8	3.8	6.7	17.9
2002	2.7	3.3	8.7	23.1
2003	3.5	4.7	16.6	44.3
2004	4.4	4.9	21.9	58.3
2005	5.3	5.5	29.2	77.8
2006	6.2	5.2	32.5	86.6
2007	6.5	5.9	38.7	103.4
2008	7.1	6.5	46.0	122.8
2009	8.0	6.4	50.8	135.6
2010	8.8	6.5	57.7	153.9
2011	8.9	7.5	66.1	176.5
2012	8.9	8.6	76.0	202.9
2013	8.9	7.8	69.1	184.6
Total			538.1	1,436.8

Note: fuel usage NT/RT = 17.3 liters/ha CT = 35 liters/ha.

Canadian canola: Potential additional soil carbon sequestration (1996 to 2013)

	Annual increase in carbon sequestered based on 1996 average (kg carbon/ha)	Crop area (million ha)	Total carbon sequestered (million kg)	Carbon dioxide (million kg)
1996	0.0	3.5	0.0	0.0
1997	3.3	4.9	15.8	58.1
1998	3.3	5.4	17.6	64.7
1999	3.3	5.6	18.1	66.4
2000	3.3	4.9	15.8	58.0
2001	6.5	3.8	24.6	90.3
2002	9.8	3.3	31.8	116.7
2003	13.0	4.7	61.0	223.7
2004	16.3	4.9	80.3	294.5
2005	19.5	5.5	107.1	393.0
2006	22.8	5.2	119.2	437.4
2007	24.1	5.9	142.2	521.7
2008	26.0	6.5	168.9	619.7
2009	29.3	6.4	186.5	684.4
2010	32.5	6.5	211.7	777.0
2011	32.5	7.5	242.8	891.1
2012	32.5	8.6	279.0	1,024.0
2013	32.5	7.8	253.9	931.8
Total			1,976.3	7,252.5

Note: NT/RT = +55 kg of carbon/ha/yr CT = −10 kg of carbon/ha/yr.

Permanent reduction in global tractor fuel consumption and carbon dioxide emissions resulting from the cultivation of GM IR cotton (1996–2013)

Table

	Total cotton area in GM IR growing countries excluding Burkina Faso, India, Pakistan, Burma and China (million ha)	GM IR area excluding Burkina Faso, India, Pakistan, Burma and China (million ha)	Total spray runs saved (million ha)	Fuel saving (million liters)	CO2 emissions saved (million kg)
1996	6.64	0.86	3.45	2.90	7.73
1997	6.23	0.92	3.67	3.09	8.24
1998	7.20	1.05	4.20	3.53	9.43
1999	7.42	2.11	8.44	7.09	18.92
2000	7.29	2.43	9.72	8.17	21.81
2001	7.25	2.55	10.18	8.55	22.84
2002	6.36	2.17	8.69	7.30	19.49
2003	5.34	2.17	8.70	7.30	19.50
2004	6.03	2.79	11.17	9.38	25.05
2005	6.34	3.21	12.84	10.78	28.79
2006	7.90	3.94	15.75	13.23	35.33
2007	6.07	3.25	12.99	10.91	29.14
2008	4.51	2.54	10.16	8.53	22.78
2009	5.33	2.96	11.83	9.94	26.54
2010	7.13	4.59	18.37	15.43	41.21
2011	6.61	4.43	17.71	14.87	39.71
2012	5.72	4.03	16.11	13.53	36.12
2013	5.35	3.80	15.21	12.77	34.10
Total			199.19	167.32	446.73

Note: assumptions: 4 tractor passes per ha, 0.84 liters/ha of fuel per insecticide application