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Articles

Evaluation of toxic effects of lambdacyhalothrin on the haematology and selected biochemical parameters of African catfish Clarias gariepinus

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Pages 45-52 | Received 19 Nov 2012, Accepted 07 Jan 2013, Published online: 12 Feb 2013
 

Abstract

The potential acute and chronic toxic effects of lambdacyhalothrin (LCT) on African catfish Clarias gariepinus were evaluated. Lethal concentrations (LC) were determined using 0.2, 0.3, 0.4, 0.5 and 0.6 ppm of LCT. Haematological and biochemical parameters were evaluated on fish treated with 0.001, 0.005, 0.010 and 0.025 ppm of LCT. Blood samples were collected on the 15th, 30th, 45th and 60th day of exposure and 60th day of recovery. Fish treated with LCT showed behavioural abnormalities compared to the control, which included reduced swimming activity, loss of equilibrium, vertical hanging and air gulping. The LC50 values of LCT were 0.571, 0.380, 0.337 and 0.325 ppm at the exposure time of 24, 48, 72 and 96 h, respectively. Significant reductions (p ⩽ 0.05) were observed in the packed cell volume, red blood cell count and haemoglobin concentration values in exposed fish. Initial increase in the value of the white blood cell count was observed, which later declined. Serum glucose increased significantly (p ⩽ 0.05), while globulin decreased significantly. Albumin did not show any significant difference among tested concentrations. The results revealed that the emulsifiable concentrate form of LCT caused reduction in haematological and biochemical parameters of C. gariepinus. The data obtained in this study provide more information on the potential impacts of LCT on non-target organisms and thus will assist in future ecotoxicological evaluations.

Straipsnyje įvertinamas ūmus ir lėtinis lambdacihalotrino poveikis afrikiniam šamui Clarias gariepinus. Letalios koncentracijos (LC) nustatytos naudojant 0,2, 0,3, 0,4, 0,5 ir 0,6 dalelių milijone (ppm) lambdacihalotriną. Žuvų biocheminiai ir hematologiniai rodikliai ištirti paveikus jas 0,001, 0,005, 0,010 ir 0,025 dalelių milijone (ppm) koncentracijos lambdacihalotrinu. Kraujo mėginiai buvo imami 15, 30, 45 ir 60 poveikio dieną bei praėjus 60 dienų po poveikio. Lambdacihalotrinu paveiktoms žuvims nustatyti elgsenos sutrikimai – sumažėjęs plaukiojimo aktyvumas, pusiausvyros praradimas, vertikali kūno padėtis ir išnirimas įkvėpti oro. Žuvis veikiant lambdacihalotrinu 24, 48, 72 ir 96 valandas, LC50 buvo 0,571, 0,380, 0,337 ir 0,325 dalelių milijone atitinkamai. Nustatytas žymus hematokrito rodiklio, raudonųjų kraujo kūnelių skaičiaus ir hemoglobino koncentracijos sumažėjimas (p ⩽ 0,05). Baltųjų kraujo kūnelių skaičiaus padidėjimas buvo stebimas tik poveikio pradžioje. Gliukozės kiekis serume ženkliai padidėjo (p ⩽ 0,05), o globulino kiekis ženkliai sumažėjo. Albuminų skaičiaus pakitimai buvo nereikšmingi. Gauti duomenys praplečia informaciją apie lambdacihalotrino poveikį organizmams ir gali būti panaudoti kitiems ekotoksikologiniams tyrimams.

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