Different Mathematical Processing of the Ratio Spectra for Quantification of Cefaclor in Presence of its Acid Induced Degradation Product

Abstract

Three simple mathematical spectrophotometric methods based on processing the ratio spectra were developed and validated for determination of cefaclor in presence of its acid induced degradation product; namely First derivative of ratio spectra (¹DD) Method (A), Ratio subtraction method (RS) Method (B) and Mean centering of ratio spectra (MCR) Method (C). The accuracy, precision and linearity ranges of the proposed methods were determined. The methods were validated and the specificity was assessed by analyzing synthetic mixtures containing the drug and its degradate. The three methods were applied for the determination of the cited drug in its pharmaceutical preparation and the obtained results were statistically compared with those of a reported method. The comparison showed that there is no significant difference between the proposed methods and the reported method regarding both accuracy and precision.

Key words:

• Cefaclor
• derivative ratio
• ratio subtraction
• mean centering