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Abstract
Vanillin, a major component of the vanilla is known to exhibit various antimicrobial properties including inhibition of bacterial biofilms. However, a systematic study on activities of vanillin against the most common fungal pathogen, Candida albicans, is lacking. In this study, broth micro dilution method was used to assess vanillin's activity on the planktonic growth of C. albicans. Minimum inhibitory concentration (MIC) was established at 1 mg ml-1 of vanillin. Prevention of yeast to hyphae morphogenesis was evident at 500 μg ml-1 of vanillin, in standard micro plate based assay. Adhesion, biofilm development and mature biofilms were studied in an in vitro biofilm model using XTTmetabolic assay and light microscopy. Significant (p < 0.05) reduction in biofilm development was observed in presence of 500 μg ml-1 vanillin. Concentration dependent decrease of cellular ergosterol in presence of vanillin indicated that inhibition of ergosterol biosynthesis may be a probable target. Our study, for the first time reveals vanillin as a potential inhibitor of biofilm formation by C. albicans.