Prevalence of O25b-ST131 clone among Escherichia coli strains producing CTX-M-15, CTX-M-14 and CTX-M-92 β-lactamases

Abstract

Background: Dissemination of multidrug-resistant Escherichia coli is closely associated with the worldwide spread of a single clone ST131, which is the main cause of urinary tract and bloodstream infections in patients from nursing homes and immunocompromised patients. The aim of our study was to determine the prevalence of ST131 clone and the replicons involved in the spread of bla_CTX-M genes among O25b-ST131 CTX-M-producing E. coli isolates in Lithuania.

Methods: The strains included in this study were screened for CTX-M β-lactamase-encoding genes, phylogenetic groups and ST131 clone by PCR. Bacterial conjugation was performed to identify plasmid replicon types responsible for bla_CTX-M genes dissemination.

Results: A total of 158 E. coli clinical non-duplicate ESBL isolates were analyzed. Nearly half (n = 67, 42.4%) of the investigated E. coli isolates belonged to phylogenetic group B2. The isolates producing CTX-M-92 β-lactamases were identified to be the ST131 clone more frequently than the non-ST131 clone (11.5% vs. 3.1%, p = .035). The CTX-M-15 isolates were identified as ST131 isolates less frequently than non-ST131 isolates (50.8% vs. 71.1%; p = .015). The ST131 clone isolates contained type L/M and A/C replicons; a fused FII/FIB replicon was found in four isolates (23.5%). Type HI1 replicon was identified in ST131 E. coli isolates producing CTX-M-15 β-lactamases.

Conclusions: This study demonstrates the predominance of the ST131 clone among CTX-M β-lactamase-producing E. coli isolates. Dissemination of bla_CTX-M genes in ST131 strains can be linked not only to highly adapted IncF plasmids such as FII/FIB and FII, but also to plasmid replicon types A/C, L/M and HI1.

Keywords:

• Escherichia coli
• ST131 clone
• CTX-M
• plasmids

Acknowledgments

The authors would like to thank Dr. V. Tatarūnas for reading the manuscript.

Disclosure statement

The authors declare that they have no competing interest.

Funding

This work was supported by grants from the Faculte de Medicine Pierre et Marie Curie (Site Saint-Antoine), Universite Paris 6, Paris, France, the Embassy of France in Lithuania, the French Institute in Lithuania, the Open Foundation of Lithuanian University of Health Sciences, and the project ‘Promotion of Student Scientific Activities’ (VP1-3.1-ŠMM-01-V-02-003), the Research Council of Lithuania (A.G.). This project was funded by the Republic of Lithuania and European Social Fund under the 2007–2013 Human Resources Development Operational Programme’s priority 3.