Abstract
Seniorwhitea reciproca belongs to the Sarcophaga genus, Sarcophagidae family and Diptera order. Here, we report the complete mitochondrial genome of S. reciproca. The circular molecule is 15,973 bp in length, consisting of A (39.80%), G (9.27%), T (36.44%), and C (14.49%), and it is composed of 22 tRNA genes, 13 protein-coding genes (PCGs), two rRNA genes, and a non-coding AT-rich region. 12 PCGs start with ATN, particularly, COI gene starting with TCG, and most PCGs use the typical stop codon TAA. Besides, the phylogenetic analyses upon mitogenomics would provide us further insights on the taxonomy and phylogeny of Sarcophagidae.
S. reciproca is a significant medical and forensic fly (Ren et al. Citation2018). It was commonly found on corpses, human excrement and decaying organic matters, they can breed on dead grasshoppers, molluscs and lepidopterous larvae (Sinha Citation2009). This species mainly spread at Asia, and it was report about the S. reciproca could cause myiasis in livestock. Mitogenome has been widely used for species identification as a molecular marker (Shang et al. Citation2018), estimations of evolutionary relationships in phylogenetic analyses and population genetics (Liao et al. Citation2016).
The specimens of S. reciproca were collected from Changsha city (28°12′N, 112°58′E), China in August 2018. Total genomic DNA was extracted from the thorax muscle and is currently stored at the Guo's Lab (Changsha city). The S. reciproca mitogenome has been submitted to GenBank with accession number is MH981944.
The complete mitogenome of S. reciproca has been obtained from high-throughput sequencing on complete mitochondrial DNA. The circular mitogenome of S. reciproca is 15,973 bp in length, contained 37 genes and an A + T rich region, which is the classical structure for Diptera. All PCGs were incorporated in 11,184 bp length, which encoding 3,728 amino acids. The 22 tRNAs were ranging from 63 bp (tRNA-Cys) to 71 bp (tRNA-Lys). Most the tRNAs of S. reciproca can be folded into the typical clover-leaf structure, whereas the tRNA-Ser (trnS1) had a special clover-leaf structure without a dihydrouridine (DHU) arm, and was replaced by a simple loop.
Phylogenetic analyses of S. reciproca with 10 Sarcophagidae species were performed based on 13 PCGs by using NJ methods, and used two Calliphoridae species were as outgroups (). The tree show S. reciproca is closely related to Sarcophaga africa, and the results of this study provided useful genetic information for further researches on phylogeny, species identification and population genetics in Sarcophagidae species.
Figure 1. Phylogenetic trees of S. reciproca with 10 Sarcophagidae species and two Calliphoridae species as outgroups, The tree based on 13 PCGs by using NJ methods.
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The authors report no conflicts of interest.
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References
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