Abstract
Sarcophaga (Liosarcophaga) dux Thomson (Diptera: Sarcophagidae) is a flesh fly species of medical and forensic importance in many parts of the world, both as a fly with the necrophagous habits found on human corpses and a vector of disease or parasitic, myiasis-producing agent. The complete mitochondria gene (mitogenome) of S. dux was first sequenced, annotated and phylogenetic analyses with other 10 species of the sarcophagidae in this study. This work improves the databases of sarcophagidae species, and provides support for further study of mitochondrial genome architecture and phylogenetics.
Sarcophaga (Liosarcophaga) dux is a significant flesh fly for medical and forensic investigations (Ren et al. Citation2018). The mitogenome has been widely used for the research of species identification and phylogenetic relationships (Fu et al. Citation2016). In this study, the complete mitogenome of S. dux was first sequenced, the full-length was a 16,153 bp fragment, consisting of A (40.0%), G (9.0%), T (37.5%), and C (13.4%), with two ribosomal RNA (rRNA) genes, 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, and a non-coding AT-rich region.
Adult specimens of S. dux were captured in Changsha (28°12′N, 112°59′E), Hunan province, China, in June 2018. After flies were frozen to death, the specimens were identified by forensic entomologist according to the key of morphological characteristics (Xu and Zhao Citation1996). The voucher specimen and its DNA were deposited in the Guo's Laboratory. The total mitogenome was extracted using the CTAB (Cetyl Trimethyl Ammonium Bromide) method (Chen et al. Citation2018). The complete mitogenome was sequenced using the combination of PCR sequencing and massively parallel DNA sequencing by an ABI PRISM 3730 automated sequencer (Applied Biosystems, Foster, CA). The S. dux mitogenome has been submitted to GenBank with accession number is MH937748.
Phylogenetic analyses of S. dux with ten sarcophagidae species were performed by neighbour-joining inference methods based on the 13 PCGs sequences, with Calliphora vomitoria, and Chrysomya pinguis as the outgroup (), showed strongly supported monophyletic groupings for 11 sarcophagidae species under five genera, which suggested that the 13 PCGs is a relatively suitable molecular marker for discrimination on some sarcophagidae species.
Figure 1. Phylogenetic analyse was constructed using neighbor joining (NJ) method based on 13 protein-coding genes.
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No potential conflict of interest was reported by the authors.
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References
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