Abstract
The complete mt genome sequence of Leiocassis longirostris was obtained by PCR, containing 37 genes with 13 protein coding genes, 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs) and a non-coding control region.
Morphological classification of Bagridae fishes is relatively difficult due to various kinds and similar shape. In this study, the complete mt genome of Leiocassis longirostris was sequenced.
Leiocassis longirostris was captured in Huai River (32°45′27″N, 116°42′37″E). The specimen is stored in Fihseries Institute, Anhui Academy of Agriculture Sciences. We amplified mitochondrial DNA using PCR method as reported (Pan and Jiang Citation2018). The complete mitochondrial genome of L. longirostris is 16,533bp (MK458524).
The genome of L. longirostris is encoded on two strands and in two directions. The genome contains a total of 37 genes, including 13 protein-coding genes, 22 transfer RNAs and two ribosomal RNAs. The 13 protein-coding genes in the mt genome of L. longirostris also share features in start and stop codons with those in other Siluriformes (Liang et al. Citation2012, Citation2013, Citation2016).
Phylogenetic analysis was performed using 13 protein-coding genes by neighbour-joining (NJ). The clade containing Leiocassis, Pelteobagrus, and Pseudobagrus is well revealed with high supporting value in the phylogenetic tree ().
Figure 1. Neighbour-joining phylogenetic tree inferred from amino acid sequence dataset of 13 protein-coding genes for 14 Siluriformes mitochondrial genomes. The tree shows the topology based on concatenated data of 13 mitochondrial encoded protein sequences. Reconstruction was performed by MEGA X (64-bit).
Acknowledgements
The authors thank Dr Maocang Yan for his comments on the manuscript.
Disclosure statement
The authors report no conflict of interest. The authors alone are responsible for the content and writing of the paper.
Additional information
Funding
References
- Liang HW, Cao L, Li Z, Zou GW, Liu XL. 2012. Mitochondrial genome sequence of the shining catfish (Pelteobagrus nitidus). Mitochondrial DNA. 23:280–281.
- Liang HW, Li Z, Zou GW. 2013. Complete mitochondrial DNA genome of Leiocassis crassilabris (Siluriformes: Bagridae)). Mitochondrial DNA. 24:222–224.
- Liang HW, Li Z, Zou GW, Shi MY. 2016. Complete mitochondrial DNA genome of Pseudobagrus medianalis (Siluriformes: Bagridae). Mitochondrial DNA: Part A. 27:587–588.
- Pan TS, Jiang H. 2018. The complete mitochondrial genome of Hebesoma violentum (Acanthocephala). Mitochondrial DNA B. 3:582–583.