Abstract
The complete mitochondrial genome of Amblyomma geoemydae is reported for the first time in this study. Its entire mitogenome is 14,780 bp in length, contained 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and two non-coding regions. The phylogenetic analysis by Maximum-likelihood method show that A. geoemydae and the others of genus Amblyomma are in the same clade, indicating that A. geoemydae belongs to the genus Amblyomma.
Ticks (Chelicerata: Anactinotrichida: Ixodida) are blood-feeding ectoparasites of terrestrial vertebrates (Burger et al. Citation2013). Amblyomma geoemydae (Ixodida: Ixodidae) is a reptile-associated tick species widely distributed the Southern part of Asia, ranging from South India to the Philippines, Australia and Japan (Bilbija et al. Citation2019). In China, it is mainly distributed in Taiwan, Jiangsu, Hunan, Hainan, and Guangxi (Sun et al. Citation2016). The polyphyly of the genus Amblyomma is relatively complex (Burger et al. Citation2013). In this study, the complete mitochondrial genome sequence of A. geoemydae was obtained, which provided a basis for the classification study of the genus Amblyomma.
The adult of A. geoemydae were collected from the bodies of a chelonian, Nanning City, Guangxi Province, China, on 18 September 2018. The individual tick was stored in the Department of Parasitology, Heilongjiang Bayi Agricultural University (specimen no. BYNKPL-180918). Species identification was conducted by professor Sun Yi based on morphological features. Primers were designed for polymerase chain reaction (PCR) amplification and sequencing on the basis of the mitogenome sequence of A. cajennense (GenBank accession no. NC_020333) (Burger et al. Citation2013).
The total length of A. geoemydae mt genome was 14,780 bp (GenBank accession no. MK814531), which contained 13 protein-coding genes (cox1-3, nad1-6, nad4L, atp6, atp8, and cytb), two rRNA genes, 22 tRNA genes, and two NCR. The arrangement of the A. geoemydae was identical with that of hard ticks (Burger et al. Citation2013; Chang et al. Citation2019). The A. geoemydae mt genome encoded 3614 amino acids in total. The concatenated amino acid sequences of 13 protein-coding genes were analyzed with Maximum-likelihood (ML), using Nuttalliellidae namaqua (NC_019663) as out group.
The result show that the tree divided into two large branches: Prostriata and Metastriata. Phylogenetic analysis revealed that the A. geoemydae and the others of genus Amblyomma are in the same clade (). This result supports traditional taxonomic assignment of A. geoemydae to the genus Amblyomma.
Figure 1. Phylogenetic relationships of Amblyomma geoemydae and other species based on mitochondrial sequence data.
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References
- Bilbija B, Auer M, Široký P. 2019. Long term persistence of introduced Amblyomma geoemydae tick population under indoor conditions in Austria. Med Vet Entomol. 33:317–321.
- Burger TD, Shao R, Barker SC. 2013. Phylogenetic analysis of the mitochondrial genomes and nuclear rRNA genes of ticks reveals a deep phylogenetic structure within the genus Haemaphysalis and further elucidates the polyphyly of the genus Amblyomma with respect to Amblyomma sphenodonti and Amblyomma elaphense. Ticks Tick-Borne Dis. 4:265–274.
- Chang QC, Diao PW, Fu X, Wang XX, Qiu YY, Hu Y, Ma XX, Sun Y, Wang CR. 2019. The complete mitochondrial genome of Haemaphysalis japonica (Ixodida: Ixodidae). Mitochondrial DNA B. 1:1006–1007.
- Sun Y, Zhang SG, Ye XD, Xu RM. 2016. Systematic classification and pictorial key of Amblyomma ticks (Ixodida: Ixodidae) in China. Acta Parasitol Med Entomol Sin. 23:111–121. (Chinese.)