Abstract
The complete mt genome sequence of Acrossocheilus wenchowensis was obtained by PCR, containing 37 genes with 13 protein coding genes, 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs) and a non-coding control region.
Morphological classification of Acrossocheilus fishes is relatively difficult due to various kinds and similar shape. In this study, the complete mt genome of Acrossocheilus wenchowensis was sequenced.
Acrossocheilus wenchowensis was captured from upstream of Xinanjiang River, Anhui Province (29°36′28″N, 118°11′33″E). Voucher specimen (SF20180628-7) was deposited in Fish Specimen Library at Fisheries Institute Anhui Academy of Agriculture Sciences, Anhui, China. We amplified mitochondrial DNA using PCR method as reported (Ai et al. Citation2013). The complete mitochondrial genome of A. wenchowensis is 16,593 bp (MN266873).
The genome of A. wenchowensis are encoded on two strands and in two directions. The genome contains a total of 37 genes, including 13 protein-coding genes, 22 transfer RNAs and 2 ribosomal RNAs. The 13 protein-coding genes in the mt genome of A. wenchowensis also share features in start and stop codons with those in other Acrossocheilus fishes (Ai et al. Citation2013; Chen et al. Citation2015; Han et al. Citation2015; Xie et al. Citation2016).
Phylogenetic analysis was performed using 13 protein-coding genes by Neighbor-Joining (NJ). The clade containing Acrossocheilus, Onychostoma, Puntius, Spinibarbus, Sinocyclocheilus is well revealed with high supporting value in the phylogenetic tree ().
Figure 1. Neighbor-Joining phylogenetic tree inferred from amino acid sequence dataset of 13 protein-coding genes in Barbinae. The tree shows the topology based on concatenated data of 13 mitochondrial encoded protein sequences. Reconstruction was performed by MEGA X (64-bit).
Acknowledgements
We thank Dr Maocang Yan for his comments on the manuscript.
Disclosure statement
The authors report no conflict of interest. The authors alone are responsible for the content and writing of the paper.
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Funding
References
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