Complete mitogenome of Reticuluma hamata (Hemiptera: Cicadellidae: Deltocephalinae) from China

Abstract

Complete mitogenome of the leafhopper species Reticuluma hamata are sequenced and assembled and annotated, the mitogenome is 15,190 bp, the base composition is 43.5% for A, 12.4% for C, 9.4% for G, and 34.7% for T. The mitogenome includes 13 protein-coding genes, 22 transfer RNA, and 2 ribosomal RNA genes. Within phylogenetic tree, all Deltocephalinae members were clustered into a clade with strongly supported. This study enriches the mitogenomes of the leafhopper.

Keywords:

• Mitogenome
• Reticuluma hamata
• phylogenetic

Reticuluma belonging to the tribe Penthimiini of the subfamily Deltocephalinae (Hemiptera: Cicadellidae), which contained 8 species until now, and exclusively distributed in China (Wang and Zhang 2019). Currently, the mitogenome of the tribe Penthimiini has been not recorded. In this study, the complete mitogenome sequences of R. hamata are the first time to determine, we hope that will apply to the studies of the identification and evolution of Penthimiini leafhoppers.

The genome DNA was extracted from adult of R. hamata which were collected from Yunnan Province, China (E 101°30′9″, N 23°57′50″), in June 2019. All the specimens were preserved in absolute alcohol and deposited in the Institute of Entomology of Guizhou University, Guiyang, China (GUGC-HCD-00113). The complete mitogenome of R. hamata forms a typical closed loop with a full length of 15,190 bp. This resource of sequence and annotation was submitted to NCBI (GenBank accession number MN922303). The composition of the mitogenome is as follows: 13 PCGs, 22 tRNA genes, 2 rRNA genes and a long non-coding region, A = 43.5%, C = 12.4%, G = 9.4%, T = 34.7%, with a total A + T content of 78.2% and is within the range reported within Hemipteran mitogenomes (68.86–86.33; Wang et al. 2015). Five PCGs (ND3, ND4, ND4L, ND6, ND1) started with ATT, four PCGs (ND2, COII, ATP6, CytB) started with ATA, two PCGs (COI, ATP8) started with ATC, two PCGs (COIII, ND5) started with ATG. COI and ND3 stopped with TAG, the remaining eleven PCGs stopped with TAA. All tRNA genes are identified by ARWEN version 1.2 software (Laslett and Canbäck 2008). The lengths of 16S rRNA gene and 12S rRNA gene are 1,190 bp and 723 bp, respectively. They are located between trnL2(CUN) and trnV, trnV and Control region, respectively. The control region is 904 bp long, and is located between 12S rRNA and trnI.

The phylogenetic relationships of R. hamata were reconstructed with IQ-TREE using an ultrafast bootstrap approximation approach with 10,000 replicates based on concatenated the nucleotides of the 13 PCGs. Each PCG sequences were aligned using the MAFFT algorithm in TranslatorX and aligned sequences were eliminated using Gblocks 9.1 b (Abascal et al. 2010). In the phylogenetic tree, all Deltocephalinae members were clustered into a clade with strongly supported (bootstrap support value = 100). The result confirms that R. hamata is part of Deltocephalinae (Figure 1). On the other hand, this study can provide molecular data for further phylogenetic and evolutionary analysis, we hope this data can be useful for further study.

Figure 1. Phylogenetic analyses of Reticuluma hamata based upon the concatenated the nucleotides of the 13 PCGs of 61 ingroup species by IQ-TREE. Numbers at nodes are bootstrap values. The accession number for each species is indicated after the scientific name.

Disclosure statement

The authors declare no potential conflict of interest was reported.

Additional information

Funding

This work was supported by the National Natural Science Foundation, China [Grant no. 31672342].