http://orcid.org/0000-0002-7426-8101
Abstract
The complete mitochondrial genome of Vibidia duodecimguttata was sequenced and reported here. The circle genome of the leaf beetle is 19,627 bp in length. There are 38 sequence elements including 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a control region. With 1 species from Salpingidae as an outgroup phylogenetic relationships of 8 Coccinellidae species based on their mitogenomes indicated that V. duodecimguttata is closest to C. championorum.
Vibidia duodecimguttata (Poda 1761) belongs to the coccinellid tribe Psylloborini, which is composed entirely of obligate consumers of various powdery mildew (PM) fungi (Sutherland and Parrella Citation2006). Vibidia duodecimguttata is widely distributed in the Palearctic region and mainly feed on the Erysiphaceae infecting broad-leaved trees and shrubs (Ceryngier and Godeau Citation2013). Since it has been reported to parasitize almost 10,000 species of angiosperm plants in 169 families (Amano Citation1986), including many crops, PM is considered one of the most important plant pathogens worldwide (Sutherland Citation2005). Ladybird beetles in the tribe Psylloborini are considered as important biocontrol agent against PM (Tezcan and Uygun Citation2003). Study on the potential of V. duodecimguttata to control PM was also conducted (Guo and Wu Citation1990).
In this study, V. duodecimguttata adult species were collected from Ledu District, Haidong, Qinghai Province, China (36°25′48″N, 102°19′46″E) in September 2019. The specimen were kept in the Insect Collection of the Entomology Lab, College of Agriculture and Animal Husbandry, Qinghai University, Xining, China (accession number: YJY-2019-JPC001). Genomic DNA was extracted using the commercial kit (Ezup Column Animal Genomic DNA Purification Kit, Sangon Biotech). Genomic sequencing was performed on the Illumina HiSeq Platform (Illumina, San Diego, CA) with a read length of 150 bp. The software SPAdes v.3.14.0 (Bankevich et al. Citation2012) was employed to assemble the mitogenome. The assembled sequence was then annotated using the web server MITOS (Bernt et al. Citation2013).
A circularized DNA assembly 19,627 bp in length was harvested and deposited in the GenBank with an accession number of MT114193. Totally, 37 genes are recognized in this mitogenome: 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, and 2 ribosomal RNA (rRNA) genes, as well as a non-coding AT-rich control region (D-loop). Of all 38 sequence elements, 4 PCGs, 9 tRNA genes, and 2 rRNA genes are located on the light strand, while others are on the heavy strand.
Based on the mitogenomes assembled here or downloaded from GenBank, phylogenetic relationships of 8 Coccinellidae species were resolved by means of neighbor-joining method with 1 species from Salpingidae as an outgroup (). After aligned using MAFFT software (Katoh and Standley Citation2013), the neighbor-joining tree was built using MEGA7 (Kumar et al. Citation2016) with bootstrap set to 1000. As indicated by the phylogenetic tree, V. duodecimguttata is closest to C. championorum.
Figure 1. The Neighbor-joining tree based on 9 mitochondrial genome sequences.
Acknowledgements
We thank Zihan Jin and Dr. Hongxia Wang for their help in collecting samples.
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No potential conflict of interest was reported by the author(s).
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References
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