Complete mitochondrial genome of Gymnocypris waddellii (Cypriniformes: Cyprinidae)

Abstract

This study provides a mitochondrial complete genome of Gymnocypris waddellii. The complete genome is 16,674 bp in length with an A + T content of 55.4%, which contains 13 protein-coding genes, 22 tRNA genes, and 2 rRNA genes. The phylogenetic analysis indicated that G. waddellii is closely related to Oxygymnocypris stewartii. These results contribute to explore the molecular biology characteristic of G. waddellii on the conditions of Qinghai-Tibet Plateau.

Keywords:

• Gymnocypris waddellii
• mitochondrial genome
• phylogenetic analysis

Gymnocypris waddellii, belongs to the genus Gymnocypris, in the subfamily of Schizothoracinae (Cypriniformes, Cyprinidae), which is the unique economic fish species on the Qinghai-Tibetan Plateau, and plays a key role in the ecosystem composed mainly of fish, birds and meadow (Wu and Wu 1991). Gymnocypris waddellii is distributed in Yangzhuoyongcuo River, Duoqin River, Zhegu River and some of the lakes and rivers of the Tibet Shannan Area (Yang et al. 2011). In order to protect the resources of the naked common carp on the Qinghai-Tibetan Plateau, some scholars research of the biological characteristics, population resources, genetic diversity and artificial reproduction domestication of the Gymnocypris przewalskii (Shi, Qi, and Yang et al. 2000, Shi, Qi, Yang, Chen, et al. 2000; Zhang et al. 2005; Jiang et al. 2009). At present, there was no research reports except Wu and Wu (1991) and Yang et al. (2011) have some research on the classification and reproductive biology of the G. waddellii. Therefore, establishing a complete mitochondrial genome sequence will contribute to explore the molecular biology characteristic of G. waddellii on the conditions of Qinghai-Tibet Plateau.

In this study, G. waddellii were collected from the Yanmdrok region (Xizang, China; 90°63′E, 29°19′N) and voucher specimens (Yanmdrok-001) were deposited at the Museum of State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University. The sample was frozen in liquid nitrogen immediately after collection and stored at –80 °C. The whole length of the complete mitogenome of G. waddellii is 16,674 bp, with an A + T content of 55.4%, and the sequence was deposited into GenBank database with accession number (MT185928). The whole mitogenome contains 13 protein-coding genes, 22 tRNA genes, and 2 rRNA genes.

Phylogenetic analysis was performed by RAxML 8.1.17 (Stamatakis 2014) based on 11 mitochondrial complete genome sequences. The results showed that G. waddellii had the highest homology with Oxygymnocypris stewartii (Figure 1).

Figure 1. The maximum likelihood tree of the Gymnocypris waddellii and 10 other fish species.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by the Natural Science Foundation of Qinghai Province of China [2020-ZJ-959Q], and the Scientific research training project of the College of Agriculture and Animal Husbandry of Qinghai University. Dr. Zhifang An and Shuo Feng were supported by ‘1000 Talent’ programs of Qinghai Province.