Abstract
Pagrus caeruleostictus is a benthopelagic marine species found in tropical regions and a member of the family Sparidae and genus Pagrus. In this article, a mitochondrial DNA sequence by high-throughput technique (HTS) was carried out on muscle of the above species and determined the complete mitogenome. The complete mitochondrial DNA sequence was 16,653 bases pairs in length (GenBank Accession number: MN319701) and comprises of 37 genes; 22 transfer RNA genes, 2 ribosomal RNA genes, 13 protein-coding genes as well as a control region as in a typical vertebrate mitochondrial DNA gene. The phylogenetic analysis showed P. caeruleostictus clustered with the clade of P. auriga.
Bluespotted seabream, Pagrus caeruleostictus is a bony fish species that belong to class Actinopterygii, order Perciformes of the family Sparidae and genus Pagrus. A total of 6 species are classified under genus Pagrus; P. caeruleostictus, P. major, P. pagrus, P. africanus, P. auratus and P. auriga (Froese and Pauly Citation2019; Kraljevic and Dulc Citation2007). Apart from P. major and P. auratus, which are native to the Northwest Pacific and Indo-Pacific respectively, (Kraljevic and Dulc Citation2007; www.fishbase.org) the remaining species are distributed in the Atlantic Ocean and the Mediterranean Sea. P. caeruleostictus is a localized species of the eastern Atlantic Ocean (Parenti Citation2019; https://www.fishbase.in/summary/4540).
In the present study, P. caeruleostictus specimen was sampled from the coast of Sekondi-Takoradi (4°54′59″N, 1° 42′06″W), Ghana, West Africa in December 2018 from the landings of a commercial fishing boat. A total of 18 specimen (muscles) (Guo et al. Citation2014) were collected and next generation sequencing was carried out on one to obtain the complete mitogenome. The samples were stored in absolute ethanol stored in −20 °C refrigerator until used. The specimen and DNA are stored in the museum of Guangdong Ocean University (#p.caeruleostictus_A2). The complete mitogenome of P. caeruleostictus was determined by high-throughput technique, Illumina Hiseq. This mitogenome (GenBank Accession number: MN319701) contains 37 genes which include 22 transfer RNA genes, 2 ribosomal RNA genes, 13 protein-coding genes in addition to a control region according to a characteristic vertebrate mitochondrial DNA gene (Landinez-Garcia et al. Citation2014).
To ascertain the evolutionary features of P. caeruleostictus mitogenomes, phylogenetic study was conducted on the complete mitochondrial sequences of other species from Order Perciformes with P. caeruleostictus.
The resultant phylogeny indicated P. caeruleostictus shares a close relationship with P. auriga. Additionally, Pagrus species together with Dentex species formed a clade based on complete mitogenome representing these genera evolved from a common ancestor. This result confirms the findings on phylogenetic relationship between Pagrus species and Dentex species (Ceruso et al. Citation2018; Mascolo et al. Citation2019, Citation2018) ().
Figure 1. Neighbor-joining (NJ) phylogenetic tree based on complete mitochondrial DNA sequences with L. fulviflamma as the out group.
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References
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