Abstract
The complete mitochondrial genome of the leafhopper Parathailocyba orla (Dworakowska, Citation1977) was sequenced and annotated in this study. The mitochondrial genome is 15,382 bp long, and nucleotide composition of the whole mitogenome is highly A + T biased (A: 45.8%, T: 33.3%, G: 8.6%, C: 12.3%). 12 PCGs have ATN as the start codon, except for atp8 gene uses TTG. All protein-coding genes used TAA as stop codon, except for nad5, cox2 and cox3, which use incomplete T–– as stop codon. Phylogenetic analysis using 13 PCGs showed that P. orla was clustered with three Typhlocybinae species, which was consistent with the conventional classification.
Parathailocyba orla (Dworakowska, Citation1977) belongs to the tribe Zyginellini of the subfamily Typhlocybinae (Hemiptera: Cicadellidae). It was established by Dworakowska in 1977 and currently includes 34 genera and 163 species worldwide (Chou and Zhang Citation1985; Dworakowska Citation1994, Citation1997; Sohi and Pathania Citation2012; Hossain et al. Citation2019; Yuan and Song Citation2019). The body length is 2.57-2.75 mm (including wing) (Zhang et al. Citation2012; Song and Li Citation2012), and they are the main pest in agriculture, forestry and animal husbandry. In this study, all examined samples were collected from Luodian County in Guizhou Province of China (25°56′N; 106°86′E). The whole body was preserved in ethanol and deposited in the insect specimen room of Guizhou Normal University with an accession number GZNU-ELS-2019010.
The whole mitochondrial genome of P. orla is 15,382 bp in size, and the total length of 13 PCGs is 10,915 bp. This mitochondrial genome was submitted to GenBank database under accession NO. MN894531. It contains 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), 2 ribosomal RNA genes (rrnL and rrnS), and an AT-rich region (D-loop). The base composition values are 45.8%, 12.3%, 8.6%, and 33.3% for A, C, G, and T, respectively, with an overall A + T content of 79.1%. The AT-skew and GC-skew of this mitogenome are 0.158 and –0.177. The shortest PCG is atp8 gene (153 bp) and the longest one is nad5 gene (1651 bp). 22 tRNA genes range in the size from 61 bp (trnA) to 72 bp (trnK). The rrnL gene is located between trnL1 and trnV, with the length of 1154 bp and A + T content of 83.88%. The rrnS gene is located between trnV and control region, with a length of 735 bp and A + T content of 82.04%. The control region is located between rrnS and trnI genes, which is 1106 bp in length with an A + T content of 92.95%.
12 PCGs have ATN as the start codon, except for atp8 gene uses TTG. All protein-coding genes used TAA as stop codon, except for nad5, cox2, and cox3, which use incomplete T––. The rrnL and rrnS of rRNA gene are 1154 bp and 735 bp, respectively. The P. orla mitogenome has a total of 55 bp intergenic spacer sequences, consisting of 8 regions, ranging in size from 2 to 22 bp. The largest intergenic spacer sequence of 22 bp is located between nad5 and trnH. Gene overlaps were found at 11 gene junctions and involved a total of 32 bp, the longest 8 bp overlapping located between trnW and trnC.
Phylogenetic analysis was based on the mitotic genomes of 16 species of insects in GenBank. The results showed that P. orla was observed closer to Typhlocyba sp. followed by Empoasca onukii and E. vitis phylogenetically among studied species of Hemiptera ().
Figure 1. Phylogenetic tree showing the relationship between P. orla and 15 other leafhoppers in inner group based on neighbour-joining method. Ascalohybris subjacens was used as the outgroup. GenBank accession numbers of each species are listed in the tree.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Data availability statement
The authors confirm that the data supporting the results of this study can be obtained from the corresponding author, upon reasonable request, or by entering the login number in GenBank.
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References
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