Abstract
Senna bicapsularis is an important flowering, ornamental plant. The extracts showed antioxidant activity and antibacterial properties. In addition, S. bicapsularis can be used as a model woody plants. In the present study, the complete chloroplast genome was sequenced. The result showed that the length of circular chloroplast genome was 162,744 bp, containing a large single-copy region of 91,176 bp, a small single-copy region of 18,264 bp, and two inverted repeat regions of 26,652 bp. The chloroplast genome contained 128 genes, including 83 protein-coding, eight rRNA, and 37 tRNA genes. Phylogenetic tree analysis showed that S. bicapsularis has closely relationship with Senna occidentalis, Senna tora and Senna siamea.
Senna bicapsularis (L.) Roxb. which belongs to Senna (Leguminosae) is one the important flowering, ornamental plant. Senna species are widely distributed in South American and tropical countries (Han et al. Citation2006). S. bicapsularis has medicinal values, and the flower extracts showed antioxidant activity and antibacterial properties (Mak et al. Citation2013). S. bicapsularis has strong adaptability and is widely used in the garden. Previous studies have shown that S. bicapsularis has cold, heat, waterlogging, and salt resistances (Lu Citation2007; Liao Citation2010; Cai and Liao Citation2017; Liu et al. Citation2019). S. bicapsularis sowed in spring, and bloomed and seeded in the same year. The growth cycle is short and can be used as model woody plants for the study of genetic variation and secondary growth. In the present study, we characterize the complete chloroplast genome of S. bicapsularis and provide basic data for studying the phylogenetic relationships in Leguminosae.
The fresh leaves of S. bicapsularis were collected from Xi’an Botanical Garden of Shaanxi Province (34°21′N, 108°95′E; Shaanxi, China), and the voucher specimen (ZY190202) was deposited in Xi’an Botanical Garden Herbarium. Chloroplast genomic DNA was extracted from the fresh leaves using the modified CTAB method (Doyle and Doyle Citation1987). Total DNA was used for the shotgun library construction and the subsequent high-throughput sequencing on the Illumina HiSeq 2500 Sequencing System.
After quality-trimmed, the obtained data were assembled using MITObim v1.8 with the reference sequence of Senna tora (GenBank: NC_030193) (Hahn et al. Citation2013). Whereafter, the genome was annotated using Geneious v9.0.2 (Biomatters Ltd., Auckland, New Zealand) by aligning with the reference chloroplast genome. The circular plastid genome map was completed using the online program OGDRAW (Lohse et al. Citation2013). The annotated chloroplast genome sequence has been deposited into the NCBI genbank (accession number: MT559309).
The total plastome length of S. bicapsularis was 162,744 bp, with large single copy (LSC, 91,176 bp), small single copy (SSC, 18,264 bp), and two inverted repeats (IRa and IRb; 26,652 bp each). The overall GC content was 36.1% (LSC: 33.6%; SSC: 30.0%; IRs: 42.4%) and the chloroplast genome contained 128 genes, including 83 protein-coding, eight rRNA, and 37 tRNA genes.
In order to investigate the phylogenetic relationship of S. bicapsularis in Leguminosae, the phylogenetic tree was constructed with MEG6 (Tamura et al. Citation2013) based on 18 complete chloroplast genome sequences and Sanguisorba officinalis (Rosaceae) (GenBank: NC_044694) was used as an outgroup (). The results indicated that compared with other genera plants of Leguminosae, S. bicapsularis has close relationship with S. occidentalis, S. tora, and Senna siamea.
Figure 1. Phylogenetic tree based on 19 complete chloroplast genome sequences in Leguminosae.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Data availability statement
The data that support the findings of this study are openly available in National Center for Biotechnology Information] at [https://www.ncbi.nlm.nih.gov/], accession number [MT559309].
Additional information
Funding
References
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