Abstract
In this study, the complete mitochondrial genome of Clavulina sp. was sequenced and assembled. The complete mitochondrial genome of Clavulina sp. contains 20 protein-coding (PCG) genes, 2 ribosomal RNA (rRNA) genes, and 25 transfer RNA (tRNA) genes. The total size of the Clavulina sp. complete mitochondrial genome is 31,816 bp, with the GC content of 27.72%. Phylogenetic analysis indicated that the mitochondrial genome of Clavulina sp. exhibited a close relationship with that of the genus Cantharellus.
The genus Clavulina is an ectomycorrhizal fungal group distributed in many countries (Olariaga et al. Citation2009; Henkel et al. Citation2011). Most of species from the Clavulina genus are edible, and some species also show medicinal values (Khaund and Joshi Citation2014; Deo et al. Citation2019). Dozens of species have been described in genus Clavulina (Thacker and Henkel Citation2004; Uehling et al. Citation2012a, Citation2012b). Limited and varied morphological characteristics make it difficult to identify and classify Clavulina species accurately only by morphology (Thacker and Henkel Citation2004; Olariaga et al. Citation2009; Uehling et al. Citation2012a, Citation2012b). Mitochondrial genome is widely used to analyze the evolution and phylogeny of species (Li, He, et al. Citation2020; Wang et al. Citation2020; Li et al. Citation2020). However, up to now, no mitochondrial genome of Clavulina species has been published. The mitochondrial genome of Clavulina sp. will promote the understanding of the evolution, phylogeny, and taxonomy of this important ectomycorrhizal fungal group.
The specimen (Clavulina sp.) was collected from a mountain in Chuxiong, Yunnan, China (101.41 E; 25.12 N). The specimen was stored in Culture Collection Center of Chengdu University (No. Clasp08). The complete mitochondrial genome of Clavulina sp. was assembled according to previously described methods (Li, Xiang, et al. Citation2019; Wang et al. Citation2020). The total genomic DNA of Clavulina sp. was extracted using a Fungal DNA Kit D3390-00 (Omega Bio-Tek, Norcross, GA). A Gel Extraction Kit (Omega Bio-Tek, Norcross, GA) was used to purify the extracted total DNA. We stored the purified DNA in Chengdu University (No. DNA_ Clasp08). Sequencing libraries were constructed with the purified genomic DNA using a NEBNext® Ultra™ II DNA Library Prep Kit (NEB, Beijing, China). We conducted whole genomic sequencing (WGS) of Clavulina sp. using the Illumina HiSeq 2500 Platform (Illumina, SanDiego, CA). The mitochondrial genome of Clavulina sp. was de novo assembled using SPAdes version 3.9.0 (Bankevich et al. Citation2012). We obtained the complete mitogenome of Clavulina sp., and then annotated it according to the methods described by Li, Chen, et al. (Citation2018), Li, Ren, et al. (Citation2019), and Li, Wang, et al. (Citation2018).
The complete mitochondrial genome of Clavulina sp. is 31,816 bp in length, with the base composition as follows: A (34.94%), T (37.32%), G (14.38%), and C (13.35%). The complete mitochondrial genome of Clavulina sp. contains 20 protein-coding genes, 2 ribosomal RNA genes (rns and rnl), and 25 transfer RNA (tRNA) genes. To investigate the phylogenetic positions of Clavulina sp., we constructed a phylogenetic tree for 20 species. The phylogenetic tree was constructed using the Bayesian analysis (BI) method based on the combined 14 core protein-coding genes according to methods described by Li, Wang, Jin, Chen, Xiong, Li, Liu, et al. (Citation2019), Li, Wang, Jin, Chen, Xiong, Li, Zhao, et al. (Citation2019) and Li, Yang, et al. (Citation2020). As shown in the phylogenetic tree (), the mitochondrial genome of Clavulina sp. exhibited a close relationship with that of the genus Cantharellus (Li, Liao, et al. Citation2018).
Figure 1. Bayesian phylogenetic analysis of 20 species based on the combined 14 core protein-coding genes. Accession numbers of mitochondrial sequences used in the phylogenetic analysis are listed in brackets after species.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Data availability statement
This mitogenome of Clavulina sp. was submitted to GenBank under the accession number of MT649302 (https://www.ncbi.nlm.nih.gov/nuccore/ MT649302).
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