Abstract
Two complete mitochondrial genomes of Gobio acutipinnatus and G. microcephalus are assembled, and they have the same length with slightly high A + T contents (54.88% and 56.57%) in base compositions. Results in the reconstructed phylogeny show that the genus Gobio is a monophyletic group, and it is a sister taxon of the genus Romanogibio.
Freshwater gudgeons in the genus Gobio (Cypriniformes: Gobionidae) include about 46 valid species (Fricke et al. Citation2020), and they are distributed in Europe and Asia (Yue Citation1998; Kottelat and Freyhof Citation2007). In this study, we assemble complete mitochondrial genomes of Gobio acutipinnatus and Gobio macrocephalus through Sanger sequencing. The two new mitochondrial genomes would be useful in species delimitation and phylogenetic reconstruction of the genus Gobio.
The specimen of G. acutipinnatus (voucher FDZM-GABu20150427-01) is collected from the Burqin County, Xinjiang Uygur Autonomous Region, China (47.71°N, 86.84°E), and G. macrocephalus (FDZM-GMWQ20170804-01) from the Wangqing County, Jilin Province, China (43.32°N, 129.78°E).
Two new mitochondrial genomes (MT632635 and MT632636 in the GenBank) have the same length (16, 609 bp), and display slightly high A + T contents (54.88% and 56.57%) in base compositions. There are two kinds of start codons (ATG and GTG) and four types of stop codons (TAG, TAA, TA– and T––) used in the 13 protein-coding genes. Gene overlaps are revealed among 6 pairs of adjoining genes with variable lengths of 1–7 bp, as well as gene intervals among 13 pairs of adjacent genes with different size of 1–31 bp. All of gene compositions, the order of genes, codon uses, overlaps and intervals among genes show similar patterns as published mitochondrial genomes of their close relatives in the family Gobionidae (Li et al. Citation2018; Chen and Fu Citation2019; Ge et al. Citation2020).
A phylogeny is reconstructed by combining with other 19 mitochondrial genomes of the family Gobionidae from the GenBank, using a maximum likelihood analysis in the IQ-TREE 1.6.2 (Nguyen et al. Citation2015). The results in our phylogeny show that the genus Gobio is a monophyletic group, and it forms a sister taxon relationship with the genus Romanogibio ().
Figure 1. Phylogenetic relationships between Gobio fishes and their relatives using 21 mitochondrial genomes based on a maximum likelihood analysis. Shown are bootstrap confidences above branches, and GenBank numbers in parentheses.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Data availability statement
New mitochondrial genomes in this study could been obtained in the GenBank with accession numbers as follows:
Additional information
Funding
References
- Chen Y, Fu CZ. 2019. Three complete mitochondrial genomes of freshwater fishes in the genus Abbottina (Cypriniformes: Gobionidae). Mitochondrial DNA Part B. 4(2):2179–2180.
- Fricke R, Eschmeyer WN, Van der Laan R. (eds). 2020. Eschmeyer’s catalog of fishes: genera, species, references; [Online version 2020 Jun 2]. http://researcharchive.calacademy.org/research/ichthyology/catalog/fishcatmain.asp
- Ge YS, Cheng QQ, Yan YB, Duan XC, Wang T, Zhang YP, Lou ZY, Du YY. 2020. The complete mitochondrial genome sequence and phylogenetic position of Gobio coriparoides. Mitochondrial DNA Part B. 5(1):808–809.
- Kottelat M, Freyhof J. 2007. Handbook of European freshwater fishes. Berlin: NHBS.
- Li YH, Cao K, Fu CZ. 2018. Ten fish mitogenomes of the tribe Gobionini (Cypriniformes: Cyprinidae: Gobioninae). Mitochondrial DNA Part B. 3(2):803–804.
- Nguyen LT, Schmidt HA, von Haeseler A, Minh BQ. 2015. IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Mol Biol Evol. 32(1):268–274.
- Yue PQ. 1998. Fauna sinica. Osteichthyes. Cypriniformes II. Beijing: Science Press.