Complete mitochondrial genome of the praying mantis Arria pallida (Zhang, 1987) (Mantodea: Haanidae)

Abstract

The complete mitochondrial genome of Arria pallida is 16,240 bp in length, containing 13 protein-coding genes (PCGs), 2 ribosomal RNA (rRNAs), a control region (D-loop), and 22 transfer RNA (tRNAs). The phylogenetic analysis included 18 species within 10 families of Mantodea using maximum likelihood (ML) method. The result showed that A. pallida is sister to Haania sp., both of which form a sister clade with Caliris sp.

Keywords:

• Mitochondrial genome
• praying mantis
• Arrini
• phylogeny

Mantodea (praying mantis) is a group of camouflaged and predatory insects, encompassing about 2500 species (Wieland and Svenson 2018). Arria pallida (Zhang 1987) belongs to Arrini within Haaniidae. Arrini is a clade of oriental praying mantis (Schwarz and Roy 2018, 2019) and it is a rare tribe with few reports. Herein, the first complete mitochondrial genome of Arria pallida and its phylogenetic relationship with other Mantises is provided. This represents the first publish genomic data of Arrini.

The A. pallida sample was collected from Fanjingshan Nature Reserve (108.65°N, 27.91°E), Guizhou province, southwest of China, during August 2018. The specimen was stored in absolute ethanol and kept in the Institute of Entomology, Guizhou University (voucher no. Ha.A-201808fj01), Guiyang, China (IEGU). Sequencing was conducted using the Illumina HiSeq platform (San Diego, CA, USA). All the genes were predicted using MitoZ (Meng et al. 2019).

The complete mitochondrial genome of A. pallida (GenBank accession MT594484, link: https://www.ncbi.nlm.nih.gov/nuccore/MT594484) is 16,240 bp in length, with base composition: 40.2% A, 38.0% T, 13.1% C, and 8.7% G. Containing 13 PCGs, two rRNA genes, a putative control region (D-loop), and 22 tRNA genes. The N-strand codes nine PCGs (nd2, co1, co2, atp8, atp6, co3, nd3, nd6, and cytb), while the J-strand codes the remaining four PCGs (nd5, nd4, nd4l, and nd1). The gene arrangement of A. pallida is similar to the common type of putative ancestor of insects. Five start codons of PCGs exist: ATG (nad2, cox2, atp6, cox3, nad5, nad4, nad4l, and cytb), TTG (cox1), ATC (nad3), ATT (nad6), ATA (atp8 and nad1). Most of the 13 PCGs had TAA as stop codon whereas cox2, cox3 had T and nad5 had TA. The rrnL is 1320 bp (81.3% AT) and the rrnS is 786 bp (79.4% AT).

Phylogenetic analysis (Figure 1) was constructed using ML method with 13 PCG sequences extracted from 18 species within 10 families using IQtree version 1.6.12 (Nguyen et al. 2015). Metallyticidae was set as outgroup. Arria pallida was found to cluster with Haania sp., both of which form a sister clade with Caliris sp. This finding is consistent with the classification system set up by Schwarz and Roy (2019).

Figure 1. Phylogenetic tree showing Arria pallida relationship with other Mantodea, based on 13 protein-coding genes.

Disclosure statement

No potential conflict of interest was reported by the author(s). The authors are responsible for the content and writing of the article.

Additional information

Funding

This work was supported by the Science and technology support program of Guizhou Province [No. 20201Y129], the Program of Excellent Innovation Talents, Guizhou Province [No. 20154021], the Program of Science and Technology Innovation Talents Team, Guizhou Province [No. 20144001], the Research Program of Guizhou Science and Technology Bureau, Guizhou Province, China [No. 黔科合基础[2018]1031], and the Research Program of New Talents Introduced to Guizhou University, China [No. 贵大人基合字[2017]18].