Abstract
The complete mitochondrial genome sequence of Poecilocoris druraei was sequenced and analyzed. The 16,524 bp mitogenome of P. druraei contained 21 tRNA genes, two rRNA genes, 13 protein-coding genes (PCGs), and a control region. Most PCGs used standard ATG start codons and complete TAA ermination codons. Phylogenetic analysis indicated that P. druraei is closed related with P. nepalensis.
Poecilocoris druraei (Linnaeus, 1771) (Hemiptera: Scutelleridae) is equipped with well-developed stinky glands and widely distributed all over the world, especially in the tropics and subtropics (Xu et al. Citation2019). Elucidating the structure and sequence of P. druraei mitogenome is important for understanding its phylogeny and evolution. Adult P. druraei was sampled from Nada County, Hainan Province, China (N 19°31′46.85″ and E 109°29′39.04″). The specimen was deposited in College of Forestry, Hainan University (Zhihang Zhuo and [email protected]; Voucher No. CFHU-PD-202006307), Haikou.
The complete mitochondrial genome of P. druraei was 16,524 bp in length (GenBank accession numbers: MZ269306), containing 13 protein-coding genes (PCGs), two rRNA genes, 21 tRNA genes, and a control region. Among them, 23 genes were encoded on the majority strand (N-strand) while another eight tRNA genes, four PCGs, and two rRNA genes were encoded on the minority strand (J-strand). The nucleotide composition was significantly biased (A, C, G, and T was 42.27%, 18.05%, 10.66%, and 31.99%, respectively), with A + T contents of 74.26%. Thirteen PCGs were identified, with a total length of 11,100 bp and distributed from 159 bp (atp8) to 1,713 bp (nad5). The shortest and longest PCGs are generally consistent with those found in other insects (Huang et al. Citation2019; Sun et al. Citation2021). Six types of start codons (ATA, TTG, ATA, ATG, ATT, and GTG) and three stop codons (TAA, TAG, T(AA)) were identified in PCGs.
To perform a comparative mitochondrial genome analysis, twelve mitochondrial genomes of Hemiptera insects were downloaded from GenBank database. Multiple sequence alignment was conducted by MAFFT v7 (Katoh and Standley Citation2013). Phylogenetic analyses were performed with maximum-likelihood in MEGA X with1000 bootstraps (Kumar et al. Citation2018). The phylogenetic tree revealed that P. druraei is closed related with P. nepalensis ().
Figure 1. The phylogenetic relationship of 12 species within the Hemiptera species based on neighbour-joining analysis of complete mitochondrial genomes. Phymata americana and Carcinocoris binghami were served as the out-group.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Data availability statement
The genome sequence data that support the findings of this study are openly available in GenBank of NCBI at (https://www.ncbi.nlm.nih.gov/) under the accession number MZ269306. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA 733298, SRR 15094403, and SAMN 20119651 respectively.
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References
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