Reconstruction of mitogenomes by NGS and phylogenetic implications for leaf beetles

Abstract

Mitochondrial genome (mitogenome) sequences are frequently used to infer phylogenetic relationships of insects at different taxonomic levels. Next-generation sequencing (NGS) techniques are revolutionizing many fields of biology, and allow for acquisition of insect mitogenomes for large number of species simultaneously. In this study, 20 full or partial mitogenomes were sequenced from pooled genomic DNA samples by NGS for leaf beetles (Chrysomelidae). Combined with published mitogenome sequences, a higher level phylogeny of Chrysomelidae was reconstructed under maximum likelihood and Bayesian inference with different models and various data treatments. The results revealed support for a basal position of Bruchinae within Chrysomelidae. In addition, two major subfamily groupings were recovered: one including seven subfamilies, namely Donaciinae, Criocerinae, Spilopyrinae, Cassidinae, Cryptocephalinae, Chlamisinae and Eumolpinae, another containing a non-monophyletic Chrysomelinae and a monophyletic Galerucinae.

Keywords:

• Next-generation sequencing
• mitochondrial genome
• phylogeny
• Chrysomelidae

Disclosure statement

The authors have declared that no competing interests exist.

Additional information

Funding

This research is supported by grants from the National Natural Science Foundation of China (No. 31402002), Key Scientific Research Projects of Henan Province (Grant Nos. 16A210029, 18B210006), Henan Academician Workstation of Pest Green Prevention and Control for Plants in Southern Henan (YZ201601), Fruit Industry System of Henan Province (S2014-11-G03), and Agricultural Science and Technology Innovation Project of Henan Province (30600309).