Abstract
The purpose of this study was synthesizing of nanoemulsion from Pistacia atlantica essential oil (PAEO-NE) and investigates its possible mechanisms in inhibiting cancer cells. Ultrasonic method was used to prepare the optimal formulation and then after characterization, its anti-cancer effects were evaluated by MTT, flow cytometry, QPCR, ROS, CAM, and scratch methods. Particles measuring 35.8 nm, PDI 0.4 were obtained with a surface charge of −32 mV in a formulation containing 9% twin 80. These particles had cytotoxic effects against skin, lung and prostate cancer cells with IC50 of less than 10 μg/mL without any toxic effect on normal cells. Increased intracellular ROS, increased expression of cas-3, 8, and IL-10 genes, inhibition of vessel length and number in CAM model and inhibition of cell migration in PAEO-NE treatment were observed. According to the results, PAEO-NE can be considered as as a safe and effective anti-lung cancer therapeutic agent.
Acknowledgments
The authors very much appreciate the support provided by the Islamic Azad University, Mashhad, Iran, in the conducting of the present research.