Biomedical applications of novel green AgNPs synthesized from endophytic bacteria Cronobacter sakazakii

Abstract

Endophytic bacterium from the flower of Nyctanthes arbor-tristiswas isolated and identified as Cronobactersakazakii. The supernatant of the biomass of this endophyte was utilized for AgNps synthesis which was confirmed by a change in color and characterized by using UV-Visible spectrophotometer, FTIR, FESEM, EDAX, Zeta potential and DLS. The antibacterial efficacy of the CS-AgNPs against Gram positive and Gram negative bacteria was determined via agar well diffusion, MIC, MBC and biofilm formation assays. The antioxidant and anti-arthritic assays were also performed. The result revealed the synthesis of the semi-spherical CS-AgNPs and showed antibacterial activity. This report is the first to reveal the isolation and identification of endophytic bacterium from the flower of Nyctanthes arbor-tristis, along its nanomaterial synthesis, characterization and biomedical applications. Our result implies that the synthesized AgNPs can be utilized as an effective antimicrobial, antioxidant and anti-arthritic agent and can be incorporated in pharmaceutical formulation for drug development.

Keywords:

• Endophytic Bacteria
• Cronobacter sakazakii
• Nyctanthes arbor-tristis
• Silver nanoparticle (AgNPs)
• Antimicrobial activity
• Reactive oxygen species (ROS)
• Anti-arthritis

Acknowledgement

LEM acknowledges MediScience International, UK, for providing scholarship for study. All authors thank B.S.Abdur Rahman Crescent Institute of Science and Technology for providing the facilities.

Authors’ contribution

SH conceived the idea, edited the manuscript. LEM conducted the experiment, analyzed data and prepared manuscript

Disclosure statement

No potential conflict of interest was reported by the author(s).