Nanocomposite smart hydrogel based on sepiolite nanochannels/N-isopropyl acrylamide/itaconic acid/acrylamide for invertase immobilization

ABSTRACT

Novel nanocomposite smart hydrogel (NSH) and smart hydrogel (SH) are prepared to be used as a support for invertase. Spectrophotometric and thermal analysis methods were used for the characterization. Lower critical solution temperature values of SH and NSH were found as 32.68°C and 30.44°C, respectively. From the pH-responsive swellings performed at three different temperatures, the inflection point values of NSH were found at higher pHs compared to SH. Invertase was immobilized onto SH and NSH (SH−I and NSH−I). The optimum pH and temperature values were found. Michaelis constant and maximum reaction rate were calculated. NSH−I showed excellent thermal, operational and pH stability.

GRAPHICAL ABSTRACT

KEYWORDS:

• In situ polymerization
• invertase
• invertase immobilization
• nanocomposite
• smart hydrogel

Disclosure statement

All authors have contributed to this manuscript, reviewed and approved the current form of the manuscript to be submitted. None of the authors has any financial interest related to this study to disclose.

Additional information

Funding

This research was supported by the Scientific Research Project Fund of Sivas Cumhuriyet University Project F−336.

Notes on contributors

Dursun Saraydın

Dursun Saraydın is a professor at the Department of Chemistry, Faculty of Science, Sivas Cumhuriyet University in Turkey. He has coauthored over 120 publications (h-factor: 32 in SCI) and has supervised several master and doctoral students. The scientific interests of Professor Saraydin’s group include the study of hydrogel synthesis via gamma-rays or a chemical initiator; hydrogel characterization; polymer adsorption (of ions, molecules, and proteins); smart hydrogels and composites; in vitro and in vivo hydrogel biocompatibility; bioethanol production with hydrogels; drug release from interpenetrating-polymer-network hydrogels; enzyme immobilization on hydrogels or composites/nanocomposites; preparation of molecularly imprinting polymers, antimicrobial and algicidal polymers, the toxic effect of monomers and image analysis of apoptosis.

H. Nursevin Öztop

H. Nursevin Öztop is a professor in the Department of Biochemistry, Faculty of Science, Sivas Cumhuriyet University. She has coauthored 36 publications (h-factor: 11 in SCI) and has supervised some master and doctoral students. Her research interests include enzymes, polymers, immobilization of proteins and cells.

Ceylan Hepokur

Ceylan Hepokur is an assistant professor in the Department of Biochemistry, Faculty of Pharmacy, Sivas Cumhuriyet University. She has coauthored 28 publications (h-factor: 5 in SCI) and has supervised many master students. Her research interests include enzymes, polymers, immobilization of proteins, molecularly imprinting polymers, antioxidants and cancer.