A Blend of Tamarindus Indica and Curcuma Longa Extracts Alleviates Monosodium Iodoacetate (MIA)-Induced Osteoarthritic Pain and Joint Inflammation in Rats

Abstract

Background and objective

NXT15906F6 (TamaFlex^TM) is a proprietary herbal composition containing Tamarindus indica seeds and Curcuma longa rhizome extracts. NXT15906F6 supplementation has been shown clinically effective in reducing knee joint pain and improving musculoskeletal functions in healthy and knee osteoarthritis (OA) subjects. The objective of the present study was to assess the possible molecular basis of the anti-OA efficacy of NXT15906F6 in a monosodium iodoacetate (MIA)-induced model of OA in rats.

Methods

Healthy male Sprague Dawley rats (age: 8–9 wk body weight, B.W.: 225–308 g (n = 12) were randomly assigned to one of the six groups, (a) vehicle control, (b) MIA control, (c) Celecoxib (10 mg/kg B.W.), (d) TF-30 (30 mg/kg B.W.), (e) TF-60 (60 mg/kg B.W.), and (f) TF-100 (100 mg/kg B.W.). OA was induced by an intra-articular injection of 3 mg MIA into the right hind knee joint. The animals received either Celecoxib or TF through oral gavage over 28 days. The vehicle control animals received intra-articular sterile normal saline.

Results

Post-treatment, NXT15906F6 groups showed significant (p < 0.05) dose-dependent pain relief as evidenced by improved body weight-bearing capacity on the right hind limb. NXT15906F6 treatment also significantly reduced the serum tumor necrosis factor-α (TNF-α, p < 0.05) and nitrite (p < 0.05) levels in a dose-dependent manner. mRNA expression analyses revealed the up-regulation of collagen type-II (COL2A1) and down-regulation of matrix metalloproteinases (MMP-3, MMP-9 and MMP-13) in the cartilage tissues of NXT15906F6-supplemented rats. Cyclooxygenase-2 and inducible nitric oxide synthase (iNOS) protein expressions were down-regulated. Decreased immunolocalization of NF-κβ (p65) was observed in the joint tissues of NXT15906F6-supplemented rats. Furthermore, microscopic observations revealed that NXT15906F6 preserved MIA-induced rats’ joint architecture and integrity.

Conclusion

NXT15906F6 reduces MIA-induced joint pain, inflammation, and cartilage degradation in rats.

Keywords:

• Monosodium iodoacetate (MIA)-induced osteoarthritis
• NXT15906F6
• joint pain and inflammation
• cyclooxygenase-2
• matrix metalloproteinases

Disclosure statement

Sae-Bom Kwon, Sangback Kim, Young-Dae Cho, Seul-Ki Kim, and Jae-Yeong Ju are employees of Kolmar BNH Co., LTD., Seoul, Korea and Gopichand Chinta, Sreenath Kundimi, and Krishanu Sengupta are employees of Laila Nutraceuticals, Vijayawada, Andhra Pradesh, India.

Additional information

Funding

The authors thank KOLMAR BNH Co., Ltd., Korea, and NXT USA, Inc., Edison, NJ, for sponsoring (grant No.2022/KBH/NXT/075) the study.